HPV vaccines 1 Flashcards
what is the job of a vaccine
provide as close a mimic to viral epitopes
what are 2 PV vaccines
OPV-mutant virus
IPV-inactivated virus
produced from live virus
can HPV replicate cells in suspension
no
what structure are viral capsid based off
simple icosahedra
what is cervarix
L1 VLP
produced in insect cells
recombinant baculovirus
truncated L1
propriety adjuvant ASO4
alum+ TLR4 ligand
MPL
protein 16/18
what is gardasil
in yeast
alum based adjuvant
VLP disassembled and assembled
16/18/6/11
what was the vaccination programme like in uk
sep 2008 girls 12-13 cervarix
3 yr catch up girls age 14-17
3 injections 6-12 months
sep 2014 - two doses sufficient
what is cytology
pap smear
detects abnormal koilocytes
what is serology
detect at DNA sequence level
name 3 other ways we can detect and diagnose HPV
in situ hybridisation
immunocytochemistry
HPV testing
what is conisation
excision of cone shaped wedge from cervix
includes trnasformation zone and part of endocervical canal
what is imiquimod
anti cancer and anti viral agent
analogue of guanosine
activate TLR7/TLR8
inflammatory response in dendritic cells
used for basal cell carcinoma or squamous cell carcinomas
what is novavax
vaccine made in insect cells
made from naonoparticles
which 2 viruses have successfully been eradicated
smallpox
rhinderpest
first generation vaccines
gardasil
cervavix
what does gardasil 9 do
agianst types HPV 16/18/31/33/35/39/45/52/58 cervical cancers
which types was epitope swapping carried out with
HPV 33/58/52
name some more accessible and cheaper vaccines methods
made in E.coli
sub unit vaccine use isolated pentamers
deliver with current vaccine
novel delivers strategy microneedles
what is the aim of therapeutic vaccines
illicit immune response in already infected person
what are aptamers
oligonucleotides
form complex structure
bind with high affinity
re foldable
modulate protein-protein interactions
how are RNA aptamers produced
SELEX in vitro selection
name 2 E7 aptamers and there effect on the cell
SiHa
from human cervical carcinoma
HPV16 genome within
expresses E6 and 7 oncoproteins
HaCaT
control cells
immortalised human keratinocytes
no virus genome within
what was found through FACS analysis
aptamers can induce apoptosis
what aptamers is approved for clinical use
macugen
what other viral proteins could be used as targets
E1 helicase inhibitor
inhibitor of interaction between E2 and host proteins
what is microwave therapy used for
podiatry and dermatology
