HPV: life cycle and replication strategy Flashcards
where can PVs mostly be found
in vertebrate species
what is the structure of the PV genome
circular 8kb double strand
what are the 2 main type of classification of PVs
alpha and beta
regarding HPV genome organisation what are early and late ORFs
early expressed in non productively infected cells and late are expressed in productively infected cells
which HPV proteins are involved in the genome maintenance
E1,E2
which proteins are involved in genome maintenance and cell proliferation
E1,E2,E5,E6,E7
which protein are involved in genome amplification
E1,E2,E4,E6,E7
which proteins are involved in virus assembly
L1,L2
go over culture methods for HPV
where does disassembly of the capsid take place
endosome
what does L2 do when HPV enters the cell
genome trafficked to cytoplasm goes to nucleus to ND10 bodies
enhances this trafficking process
what does the early promoter do
P97 in undifferentiated cells directs expression of E6 and 7
where are late promoters
in differentiated cells drives expression of E4,L1,L2,E1
what role does LCR play
initial expression
virus latency
enhancer elements
binding site for E2
what do E2 proteins contain
DNA and dimerisation domain
transactivating domain in amino terminus
