HIV & Hepatitis Flashcards

1
Q

HIV genome

A

2 copies ssRNA, negative-sense

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2
Q

HIV - 2 important glycoproteins

A

gp41 & gp120

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3
Q

HIV - 3 important enzymes

A

Protease, RT, integrase

-all in nucleocapsid

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4
Q

HIV - 3 genes and what each encodes

A

gag - p24 (capsid), p17, p6
pol - RT (p51), p66
env - gp41, gp120

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5
Q

This HIV appears early in the infection. Why is it not a good confirmatory protein?

A

p24

p24 disappears because host develops immunity to it

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6
Q

HIV - spread mainly through… Anything covered in ___ is more infectious

A

Sexual contact

Blood
-HIV in all bodily fluids, but blood is especially infectious

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7
Q

Healthcare workers can be exposed to HIV through

A

Prick

  • 1 in 300 workers
  • also splash, exposure, etc
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8
Q

Why does having an preexisting STD increase likelihood for HIV?

A

Lots of inflammation and CD+ cells in the area

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9
Q

HIV binds to this receptor… Requires these co-receptors

A

CD4

CXCR4 = T cells
CCR2 & CCR5 = macrophages

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10
Q

HIV - Where does integrase cut the host genome?

A

Anywhere

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11
Q

Why is it hard to make vaccines for HIV?

A

Antigenic variation

-HIV is an RNA virus, lacks proofreading mechanism

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12
Q

HIV - clinical manifestations

A

Hypergammaglobulinemia

Increase p24 (initially)

Decreased CD4 - less lymphocytes

<200 cells / ul

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13
Q

HIV - screening & confirmatory tests

A

ELISA for gp120

Western blot for at least 2 out of 3 bands

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14
Q

A patient has HIV when their CD4+ cell count drops below… What’s the normal CD4:CD8 ratio? What’s the ratio for an HIV+ person?

A

<200 cells / ul

2:1

<1:1

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15
Q

What kind of HIV testing should be performed on an infant?

A

RT-PCR

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16
Q

HIV - What are some downsides of using ELISA testing?

A

False positive or false negative

-expired kits, handling, pre-analytical, patient in window period

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17
Q

Process for confirmation HIV+ patient

A

ELISA for gp120
Second ELISA to confirm
Western blot for 2/3 of these proteins - p24, gp41, gp120/gp160

18
Q

Advanced liver disease results in…

A

Cirrhosis (scarring)

-cannot be reversed

19
Q

Liver disease - clinical presentations

A

Jaundice

Urine/feces color change

Appetite loss

Cold/flu-like symptoms

Liver tenderness

Hepatomegaly

20
Q

Why does liver damage cause dark urine?

A

Bilirubin from broken down RBCs

  • highly toxic
  • brain damage if accumulates
21
Q

Liver disease - lab findings (3)

A

Elevated Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Bilirubin

22
Q

Hepatitis - how many types are there? Which ones are DNA and which ones are RNA?

A

5
A through E

B is DNA, rest are RNA

23
Q

Antibodies are needed against Hepatitis B to ___ the virus

A

Neutralize

24
Q

A recent Hepatitis infection shows elevated levels of ___. After 6 months, elevated levels of ___ are detected

A

IgM

IgG & IgA

25
Q

Vaccinating against Hep B also protects you from…

A

Hep D

26
Q

Hep B and Hep D infecting at the same time is called… Hep D infecting after Hep B is called

A

Coinfection

Superinfection - high chance of chronicity

27
Q

Hep A - acute infection marker is…

A

anti-HAV - IgM antibody directed against Hepatitis A (HAV)

28
Q

What are the antibody markers for Hep B?

A

anti-HBc

anti-HBe

anti-HBs

29
Q

Hep B - the two core proteins are

A

Core protein

E protein

30
Q

Hep B - markers that appear in the blood in order

A

HBs Ag - surface antigen

HBe Ag - E protein

HBc Antibody

HBe Antibody

HBs Antibody

-mnemonic = “seces” (pronounced sexist)

31
Q

anti-HBc IgG in the blood indicates what?

A

Person had a Hep B infection, but not a Hep B vaccination

  • vaccination targets HBs
  • IgG indicates recovery from disease & an infection that happen awhile ago
32
Q

HBe and HBs antigens in the blood after 6 months indicates what?

A

Infected person never recovered from acute infection. Infection becomes chronic
-anti-HBc IgM and total anti-HBc do develop

33
Q

High HBe antigens with no anti-HBe antibodies in the blood indicates what?

A

Infected person is not recovering. Very severe

  • HBe = indicates high degree of infectivity
  • anti-HBe = person is recovering
34
Q

Vaccinated people only have this antibody in their blood

A

anti-HBs antibody only

35
Q

anti-HBs antibody in the blood indicates what?

A

Person is infected, resolved their infection, or had a vaccination

36
Q

Chances of developing chronic liver disease when infected with Hep B? Hep C?

A

The younger you are when you get a Hep B infection, the more likely you will get cirrhosis or chronicity later in life

Hep C = 90% will develop chronic liver disease regardless of age

37
Q

Hep B monitoring panel consists of 4 things

A

HBs Ag
anti-HBs antibody
HBe Ag
anti-HBe antibody

-screen, diagnose, monitor

38
Q

Which two Hep B markers are acute markers?

A

HBs antigen
HBe antigen
-both appear and decline < 6 months

39
Q

Once this antibody appears during an acute Hep B infection, the person is considered “protected”

A

anti-HBs

-anti-HBe will develop around same time to resolve infection in blood

40
Q

Hep C - acute infection marker is

A

anti-HCV

41
Q

Hepatitis transmission routes

A

Hep A, Hep E = oral-fecal
-mnemonic = “AtE”

Hep B, Hep C, Hep D = parenteral