HIV & Hepatitis Flashcards
HIV genome
2 copies ssRNA, negative-sense
HIV - 2 important glycoproteins
gp41 & gp120
HIV - 3 important enzymes
Protease, RT, integrase
-all in nucleocapsid
HIV - 3 genes and what each encodes
gag - p24 (capsid), p17, p6
pol - RT (p51), p66
env - gp41, gp120
This HIV appears early in the infection. Why is it not a good confirmatory protein?
p24
p24 disappears because host develops immunity to it
HIV - spread mainly through… Anything covered in ___ is more infectious
Sexual contact
Blood
-HIV in all bodily fluids, but blood is especially infectious
Healthcare workers can be exposed to HIV through
Prick
- 1 in 300 workers
- also splash, exposure, etc
Why does having an preexisting STD increase likelihood for HIV?
Lots of inflammation and CD+ cells in the area
HIV binds to this receptor… Requires these co-receptors
CD4
CXCR4 = T cells
CCR2 & CCR5 = macrophages
HIV - Where does integrase cut the host genome?
Anywhere
Why is it hard to make vaccines for HIV?
Antigenic variation
-HIV is an RNA virus, lacks proofreading mechanism
HIV - clinical manifestations
Hypergammaglobulinemia
Increase p24 (initially)
Decreased CD4 - less lymphocytes
<200 cells / ul
HIV - screening & confirmatory tests
ELISA for gp120
Western blot for at least 2 out of 3 bands
A patient has HIV when their CD4+ cell count drops below… What’s the normal CD4:CD8 ratio? What’s the ratio for an HIV+ person?
<200 cells / ul
2:1
<1:1
What kind of HIV testing should be performed on an infant?
RT-PCR
HIV - What are some downsides of using ELISA testing?
False positive or false negative
-expired kits, handling, pre-analytical, patient in window period