BB - Day 3 Flashcards

1
Q

Kell low frequency antigen(s)

A

Kp(a)

Js(a)

-both can be ruled out by single heterozygous crossout

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2
Q

Lutheran low frequency antigen(s)

A

Lu(a)

  • also an IgM = clinically insignificant
  • can be ruled out by single heterozygous crossout
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3
Q

Can M and N antigens be destroyed by enzymes? Can S and s antigens be destroyed by enzymes?

A

Yes

  • sits on outer edge of glycoprotein
  • easily destroyed by enzymes

Variable
-antigens further down glycoprotein, less accessible

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4
Q

anti-M and anti-N antibodies belong to what class of antibodies? Do they bind complement?

A

IgM mostly
-cold reactive

Do not bind complement

-clinically insignificant

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5
Q

anti-S and anti-s antibodies belong to what class of antibodies? Do they bind complement?

A

IgG

Yes
-hemolytic transfusion reactions

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6
Q

This ANTIGEN varies in strength in adults

A

P1 antigen

-only antigen in the P group

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7
Q

anti-P1 antibodies belong to what class of antibodies?

A

IgM

-reactive at 4C

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8
Q

Does P1 antibody cause HDFN?

A

No

  • IgM antibody, but antigen is poorly developed
  • clinically insignificant
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9
Q

I blood group - are I and i antigens antithetical? Where can each antigen be found?

A

No

Babies have little i at birth
-up to 18 months, then decrease

Adults have big I
-trace amounts of little i

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10
Q

anti-I antibodies belong to what class of antibodies?

A

IgM

  • cold auto-antibody
  • clinically insignificant
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11
Q

anti-k (little k) is also called…

A

Cellano

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12
Q

What are the antithetical Kell group antigens?

A

K and k
Kp(a) and Kp(b)
Js(a) and Js(b)

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13
Q

Can Kell be denatured by enzymes?

A

No - no effect

-reaction strength stays the same

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14
Q

This antigen is the second highest in immunogenicity after D antigen

A

K

  • Kell antigens are highly immunogenic
  • multiple transfusions increase incidence
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15
Q

anti-K antibodies belong to what class of antibodies?

A

IgG

  • AHG by IAT (Coombs)
  • clinically significant
  • K requires two heterozygous crossouts to rule out
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16
Q

Which antigen blood groups can be destroyed by enzymes?

A

Duffy

M and N
-S and s are variable

17
Q

The antibodies in this antigen group are usually found with another antibody and not by themselves

A

Duffy

18
Q

anti-Fy(a) and anti-Fy(b) antibodies belong to what class of antibodies?

A

IgG

  • react best at AHG
  • saline agglutinins
  • clinically significant
19
Q

Which groups of antigens are enhanced by enzymes?

A
ABO
Rh
Hh
Lewis
Kidd
I
Pi

-mnemonic = “KALIPH Rh” or “Rich Kidd Lewis P. the I”

20
Q

This blood group is notorious for falling below detectable limits

A

Kidd

-anti-Jk(a) and anti-Jk(b)

21
Q

Which groups of antigens are IgM?

A
ABO
MN (no S or s)
P1
Ii - I and i
Lewis - Le(a) and Le(b)
Lutheran - Lu(a) only
  • mnemonic = “ABOM-N-PILLa”
  • IgM = not clinically significant, except ABO
22
Q

Naturally-occurring antibodies include…

A
anti-A
anti-B
anti-Cw
anti-M
Ii system antibodies
Lewis system antibodies
P system antibodies

-mnemonic = “ABC LIMP”

23
Q

Enzymes have no effect on these antigen groups

A

Kell

Lutheran

24
Q

anti-Lu(a) antibodies belong to what class of antibodies? anti-Lu(b) antibodies belong to what class of antibodies?

A

IgM

  • clinically insignificant
  • room temp

IgG

  • clinically significant
  • implicated in shortened RBC survival
  • 37C
25
Q

This blood system produce soluble antigens. The antigens are not made by RBCs, but rather are made in…

A

Lewis - Le(a) and Le(b)

  • not integral part of RBC membrane
  • primarily in secretions and plasma

Tissue

26
Q

During pregnancy, soluble levels of Lewis antigens ___

A

Decrease

-fluid gain during pregnancy cause dilution

27
Q

Lewis systems depends on these 3 genes to produce Lewis antigen structures

A

H
Se (secretor)
Le (Lewis)

28
Q

Lewis - what does the Se gene enable to H gene to do?

A

H gene is a transferase that acts in secretions

29
Q

Does the Le(a) antigen depend on the secretor gene (Se) in order to be made? Why or why not?

A

No

If Le gene is inherited, Le(a) is made and in secretions regardless of secretor status
-Le(a) adsorbed onto RBCs

30
Q

Does the Le(b) antigen depend on the secretor gene (Se) in order to be made? Why or why not?

A

Yes - both Se gene and Le gene are needed for Le(b) to be made

Lewis transferase converts converts soluble H to Le(b) antigen
-RBCs adsorb Le(b) instead of Le(a)

31
Q

If a person inherits both le/le genes from their parents, what antigen(s) are present on their RBCs?

A

Neither Le(a) nor Le(b)

32
Q

Lewis antibodies belong to what class of antibodies?

A

IgM

-clinically insignificant

33
Q

Antibodies that are clinically significant include the blood groups…

A
ABO (only IgM group)
Rh
S and s (M and N are not)
Kell
Kidd
Duffy
Lu(b) - Lu(a) is not significant
34
Q

Which blood group systems can trigger complement and cause intravascular hemolysis?

A

Kidd

Duffy

S and s (M and N do not)

Le(b)

35
Q

Why does Kell (big K) always occur as heterozygous and not by itself as homozygous?

A

Cellano (small k) is present is almost everyone, so when big K is present, Cellano is present as well, making the person heterozygous