BB - Blood Components & Donor Selection Flashcards
The label print on a blood component bag must be in this color
Black and white
What are the end results from doing a slow spin and a hard spin on whole blood?
Slow spin = packed RBCs (pRBC), platelet rich plasma (PRP; platelet concentrate)
Hard spin = packed RBCs, fresh frozen plasma (FFP), cryoprecipitate (CryoPP), cryosupernatant (Cryosuper; plasma cryoprecipitate reduced)
Whole blood units contain these 3 components
Red cells
White cells
Platelets (in plasma)
Whole blood and RBC/RBC-LR - storage temp and shelf life for units that were never frozen? How much longer can they be stored with an additive?
1-6C for 35 days (CPDA-1)
-ACD, CPD, CP2D - 21 days
42 days (1 more week)
What is the dosage bump for a 70kg adult given 1 unit of whole blood/RBC/RBC-LR?
hct increase 3%
hgb increase 1 g/dl
If a RBC unit is broken during processing, what is the new shelf life?
24 hours
- must be transfused within this time
- new expiration date/time must be on label and records
What is in a RBC-LR unit?
Red cells with white cells reduced
Frozen RBCs contain this additive to prevent them from rupturing when frozen… Once thawed, the unit is called…
Glycerol
Deglycerolized RBCs
-glycerol removed and saline added
Frozen RBCs need to be stored at what temperature? Shelf life? Once thawed, they need to be kept at this temperature and has this shelf life…
-65C or below
10 years
1-6C for 24 hrs
What is the dosage bump for a 70kg adult given 1 unit of thawed (deglycerolized) RBCs?
hct increase 3%
hgb increase 1 g/dl
-same as other units
FFP needs to be prepared and frozen within… FFP contains…
8 hours of whole blood phlebotomy
Plasma proteins
All coagulation factors
-poor in platelets (use platelet-rich plasma instead)
FFP/FP24/PF24RT24 - storage temp and shelf life for frozen units? Storage temp and shelf life for thawed units?
-18C or below for 1 year
1-6C for 24 hours
What are FP24 and PF24RT24?
FP24 = Whole blood and plasma prepared and FROZEN within 24 hours of phlebotomy; stored in fridge within 8 hours of phlebotomy
PF24RT24 = apheresis plasma prepared within 24 hours after phlebotomy. Held at room temp up to 24 hours after phlebotomy
Cryoprecipitated antihemophilic factor (AHF; cryo) - contents?
Cold insoluble precipitate from thawed FFP
-rich in Factor VIII, XIII, vWF, fibrinogen
Cryoprecipitated antihemophilic factor (AHF; cryo) - storage temp and shelf life for frozen units? Storage temp and shelf life for thawed units?
-18C or below for 1 year
Room temp for 6 hours, or 4 hours if pooled
Plasma cryoprecipitate reduced (cryosuper) - contents? How long can it be kept out before being refrozen? Frozen at what temp?
Residual plasma from cryoprecipitate production
- deficient in Factor VIII, Factor XIII, vWF, fibrinogen
- has other clotting factors
24 hours
-18C or colder
Difference between the contents of AHF cryo and cryosuper?
AHF cryo is rich in Factor VIII, XIII, vWF, fibrinogen whereas cryosuper is deficient in those proteins
-cryosuper still has other clotting factors
Cryosuper - storage temp and shelf life for frozen units? Storage temp and shelf life for thawed units?
-18C or below for 1 year
1-6C for 5 days
Platelets - storage temp and shelf life? Platelets storage require ___ to ensure platelets are oxygenated and CO2 is expelled
20-24C (room temp), 5 days
Agitation
Platelets - for an adult, how many bags are needed? Alternative?
5-6 concentrates (PLT, PLC)
-1 concentrate = 10 kg body weight
1 single donor platelets; platelet pheresis (SDP, PP)
Platelets - what is the dosage increase from concentrate and from platelet pheresis?
5,000-10,000/ul
30,000-60,000/ul
Granulocytes - contents. Storage temp and shelf life
Concentrated leukocytes
-very rarely ordered
20-24C (room temp), transfuse ASAP within 24 hours of collection
Purpose of irradiation? Prevents what disease?
Stops WBC proliferation
Prevents GVHD in immunodeficient patients
Irradiated components - shelf life and temp
28 days from irradiation, but not more than original expiration date (ie platelets expire in 5 days)
Original component’s storage temp
- RBCs = 1-6C
- platelets = room temp
