histotech Flashcards
Excess fluid in abdomen
Ascites fluid
Excess fluid in pleura
pleural effusion fluid
Deals with the preparation of the tissue for microscopic examination
Histological technique
Years of preservation of tissue
10 years
It is the process by which cells and tissue are fixed in a physical and chemical state
Fixation
The volume of the specimen should be approximately
10 to 20 times
Fixative materials that provide balance between those ingredients that cause shrinkage of the sale and those cause swelling of the cell
bouins fluid
It is the destruction of the cells or tissues by their own enzyme
Autolysis
The chemical that is cheap rapid and tends to harden tissue on prolong application
xylol or Xylene
Chemical that makes the tissue less brittle than xylol it causes shirinkage on prolong use
Chloroform
It is a glass knife mounted in and microtome
Light microscopy
It is a diamond knife in an ultramicrotome
TEM
Light microscopy slice into
4 to 6 µm
TEM slice into
50 nm
This process is done to pull the water from sample
dehydration
Melting point temperature of paraffin wax
50 to 60°C
Volume of walk should be about
25 to 30 times
Most common tissue adhesive
Eggs albumin or mayers egg albumin
It is used for the spot quickly diagnosis
Frozen section biopsy
AFreezing microtome or
Cryostat machine
Cryostat machine temperature
-20 to -30
thickness in frozen section biopsy
5 to 10 microns
Disease or illness in frozen section biopsy
Breast cancer, malignant skin lesion, brain tumors
It is to remove deposits of calcium from tissues
decalcification
It is a mild decalcifying agent
Normal saline
Fluids for decalcification
Normal saline and nitirc acid materials
Fluid that is effective at decalcifying tissues
nitric acid materials
It divides forming two new identical cells
Binary fission
Binary fission in
Prokaryotes
It’s the process by which a cell divides to form two new cells
Cell division
