histotech Flashcards
Excess fluid in abdomen
Ascites fluid
Excess fluid in pleura
pleural effusion fluid
Deals with the preparation of the tissue for microscopic examination
Histological technique
Years of preservation of tissue
10 years
It is the process by which cells and tissue are fixed in a physical and chemical state
Fixation
The volume of the specimen should be approximately
10 to 20 times
Fixative materials that provide balance between those ingredients that cause shrinkage of the sale and those cause swelling of the cell
bouins fluid
It is the destruction of the cells or tissues by their own enzyme
Autolysis
The chemical that is cheap rapid and tends to harden tissue on prolong application
xylol or Xylene
Chemical that makes the tissue less brittle than xylol it causes shirinkage on prolong use
Chloroform
It is a glass knife mounted in and microtome
Light microscopy
It is a diamond knife in an ultramicrotome
TEM
Light microscopy slice into
4 to 6 µm
TEM slice into
50 nm
This process is done to pull the water from sample
dehydration