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HISTOPATH > HISTOPATH: FIXATIVES > Flashcards

HISTOPATH: FIXATIVES Flashcards

1
Q

TYPES OF FIXATIVES ACCORDING TO COMPOSITION

A

Aldehyde fixatives
Metallic fixatives
Picric acid fixatives
Alcohol fixatives
Osmium Tetroxide

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2
Q

Types of Aldehyde fixatives use for lipids

A

Formol-calcium

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3
Q

Types of Aldehyde fixatives use as the best general fixatives that has pH of 6.8

A

10% neutral buffered formalin

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4
Q

Types of Aldehyde fixatives use for the preservation of lipids

A

10% Formol saline

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5
Q

Types of alcoholic fixatives use for chromosomes, lymph glands and urgent biopies

A

Carnoy’s fluid

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6
Q

Fixatives use for frozen sections

A

Clarke’s solution

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7
Q

Used fot mucopolysaccaride

A

Newcomer’s fluid

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8
Q

For embryo and pituitary biopsies

A

Bouin’s solution

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9
Q

Used for GIT and endocrine specimen

A

Hollande’s solution

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10
Q

It solidifies at 17°C

A

Glacial acetic acid

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11
Q

Recommended for acid mucopolysaccaride

A

Lead fixatives

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12
Q

Stains for electric microscope

A

Uranyl Acetate
Phosphotungstic acic
Lead

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13
Q

It is a mixture of 70% ethanol and 30% ammonia water then wash with water

A

Kardesewitsch method

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14
Q

Needle biopsies, 2-4 hours room temp

A

2.5% glutaraldehyde

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15
Q

Cytoplasmic stain

A

Flemming’s solution without acetic acid

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16
Q

Most common metallic fixative

A

Mercuric chloride

17
Q

Major disadvantage of using mercuric chloride

A

Causeses tissue to shrink

18
Q

Use for small pieces of liver, spleen, connective tissue fibers and nuclei

A

Zenker’s fluid

19
Q

Zenker-formol (Helly’s)

A

Pituitary glands and bone marrow

20
Q

Tumor biopsies esp the skin

A

Heidenhain’s susa

21
Q

Major disadvantage of osmium tetroxide

A

Inhibits hematoxylin

22
Q

Preserve carbohydrates

A

Chromic acid

23
Q

Early degenerative process and tissue necrosis

A

Orth’s fluid

HISTOPATH (6 decks)

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