HISTOPATH: FIXATIVES Flashcards
TYPES OF FIXATIVES ACCORDING TO COMPOSITION
Aldehyde fixatives
Metallic fixatives
Picric acid fixatives
Alcohol fixatives
Osmium Tetroxide
Types of Aldehyde fixatives use for lipids
Formol-calcium
Types of Aldehyde fixatives use as the best general fixatives that has pH of 6.8
10% neutral buffered formalin
Types of Aldehyde fixatives use for the preservation of lipids
10% Formol saline
Types of alcoholic fixatives use for chromosomes, lymph glands and urgent biopies
Carnoy’s fluid
Fixatives use for frozen sections
Clarke’s solution
Used fot mucopolysaccaride
Newcomer’s fluid
For embryo and pituitary biopsies
Bouin’s solution
Used for GIT and endocrine specimen
Hollande’s solution
It solidifies at 17°C
Glacial acetic acid
Recommended for acid mucopolysaccaride
Lead fixatives
Stains for electric microscope
Uranyl Acetate
Phosphotungstic acic
Lead
It is a mixture of 70% ethanol and 30% ammonia water then wash with water
Kardesewitsch method
Needle biopsies, 2-4 hours room temp
2.5% glutaraldehyde
Cytoplasmic stain
Flemming’s solution without acetic acid
Most common metallic fixative
Mercuric chloride
Major disadvantage of using mercuric chloride
Causeses tissue to shrink
Use for small pieces of liver, spleen, connective tissue fibers and nuclei
Zenker’s fluid
Zenker-formol (Helly’s)
Pituitary glands and bone marrow
Tumor biopsies esp the skin
Heidenhain’s susa
Major disadvantage of osmium tetroxide
Inhibits hematoxylin
Preserve carbohydrates
Chromic acid
Early degenerative process and tissue necrosis
Orth’s fluid
