DECALCIFICATION

Question 1

Removal of calcium

Answer 1

Decalcification

Question 2

What is the volume for decalcification?

Answer 2

20x

Question 3

Specimen for decalcification

Answer 3

Bone
Teeth
Tuberculous lung
Atherosclerotic blood vessel

Question 4

ACID DECALCIFYING AGENTS

Answer 4

Nitric acid
10% Aqueous nitric acid
Formol-nitric acid
Perenyi’s fluid
Phloroglucin-nitric acid

Question 5

Most common qnd fastest acid decalcifying agent

Answer 5

Nitric acid

Question 6

Used for heavily mineralized cortical bone

Answer 6

10% aqueous nitric acid

Question 7

Softener and decalcifying agent

Answer 7

Perenyi’s fluid

Question 8

For small pieces of bone and teeth

Answer 8

Von ebner’s fluid

Question 9

Autopsy materials, bone marrow, cartilage and tissues for research purposes

Answer 9

Formic acid

Question 10

Excellent bone decalcifier for enzyme

Answer 10

edta

Question 11

Removes calcium ions from formic acid-containing decalcifying solution

Answer 11

Ion exchange resin

Question 12

Calcium migrates towards the negative pole

Answer 12

Electrophoresis

Question 13

Factors influencing rate of decalcification

Answer 13

Concentration
Volume
Fluid access
Size and consistency
Agitation
Temperature

Question 14

Tissue softeners

Answer 14

4% aqueous phenol
Molliflex
2% hydrogen chloride
1% hydrogen chloride in 70% alcohol

Question 15

Test to measure the completeness of decalcification

Answer 15

Physical or mechanical
X-ray
Chemical Method (Calcium oxalate test)