DEHYDRATION-CLEARING-IMPREGNATION

Question 1

Removal of intercellular and extracellular water from tissue

Answer 1

Dehydration

Question 2

Routine dehydrating with

Answer 2

70% ethanol

Question 3

Embryonic tissue starts with

Answer 3

30% ethanol

Question 4

Volume of dehydrating

Answer 4

10x

Question 5

Ratio of dehydrating agent and tissue

Answer 5

10:1

Question 6

They are both dehydrating and clearing agent

Answer 6

Diethylene dioxide and Tetrahydrofuran

Question 7

Plants and animals

Answer 7

Butanol

Question 8

Urgent biopsies

Answer 8

Acetone

Question 9

Miscible with alcohol , toluene, and xylene

Answer 9

Petanol

Question 10

Blood and tissue films

Answer 10

Methanol

Question 11

Best dehydratinf solutions

Answer 11

Ethanol

Question 12

Removal of alcohol from tissue and replace with a substance that will dissolve the wax

Answer 12

Clearing

Question 13

What should be the refraction index

Answer 13

1.518

Question 14

Most rapid clearing agent

Answer 14

Xylene

Question 15

Carcinogenic and can cause aplastic anemia

Answer 15

Benzene

Question 16

Better at preserving tissues but more expensive

Answer 16

Toluene

Question 17

Turns milky if incomplete dehydration

Answer 17

Xylene

Question 18

Used as oil immersion

Answer 18

Cedarwood oil

Question 19

For tough tissue speimen, nervous tissue, lymph nodes and embryo

Answer 19

Chloroform

Question 20

For embryo, insects and very delicate specimen

Answer 20

Aniline oil

Question 21

Causes minimum tissue shrinkage

Answer 21

Clove oil

Question 22

Similar to chloroform but cheaper

Answer 22

Carbon tetrachloride

Question 23

Removal of clearing agent and replaced by a medium that wil completely fill all tissue cavities

Answer 23

Impregnation

Question 24

Methods of impregnation and embedding

Answer 24

Manual processing
Automatic processing
Vacuum embedding

Question 25

Fastest method of impregnation

Answer 25

Vacuum embedding

Question 26

Four changes of wax every 15 mins

Answer 26

Manual processing

Question 27

Four types of impregnating and embedding medium

Answer 27

Paraffin Celloidin Gelatin Plastic

Question 28

Simplest and most common, best embedding medium

Answer 28

Paraffin wax Impregnation

Question 29

Reduces infiltration and facilitates thin sectioning

Answer 29

Dimethyl sulphoxide

Question 30

Contains rubber

Answer 30

Tissue mat

Question 31

Used for impregnation without clearing, melting point 46-48 °C

Answer 31

Ester wax

Question 32

More elastic and resilient, MP: 56-57°C

Answer 32

Paraplast

Question 33

MP: 57-58°C

Answer 33

Embeddol

Question 34

For the eyes

Answer 34

Bio/aid

Question 35

Used for bones teeth and brains

Answer 35

Wet celloidin method