HISTOPATH

Question 1

What is the process done following complete infiltration?

Answer 1

Embedding

Question 2

What method of smear preparation maintains cellular interrelationships of the material to be examined

Answer 2

Spreading

Question 3

Which part of the microscope supports and holds the magnifying and adjustment system?

Answer 3

Arm

Question 4

It is a colorless clearing agent that is commonly used in histology laboratory and the clearing time is usually 1/2 to 1 hour

Answer 4

Xylene

Question 5

It it a very weak decalcifying solution suitable only for minute pieces of bone?

Answer 5

Sulfurous Acid

Question 6

It is an excellent dehydrating and clearing agent readily miscible in water, melted paraffin alcohol and xylene

Answer 6

Dioxane

Question 7

What method of fresh tissue examination is recommended when lipids and nervous tissue elements are to be demonstrated?

Answer 7

Frozen section

Question 8

Which of the following stains blue with H and E stain?

Answer 8

Nuclei

Question 9

Immersion oil should be removed immediately after use from the microscope lenses using what materials?

Answer 9

Lens Paper
Xylene

Question 10

As a general rule, what is the amount of dehydrating age used during dehydration process?

Answer 10

Greater than the volume of the tissue

Question 11

Water-rich tissue are hardened by freezing and cut in the frozen state using what instrument?

Answer 11

Cryostat

Question 12

What procedure is done to flatten the tissue sections and prepare tissue from mounting into the slide?

Answer 12

Floatation

Question 13

This term may also be referred to a as de-alcoholization

Answer 13

Clearing

Question 14

Serous effusions refers to the fluid accumulated in the three serous cavities namely;

Answer 14

Peritoneal
Pericardial
Pleura

Question 15

What fixative used to hemolyze the red blood cells in hemorrhagic samples?

Answer 15

Carnoy’s fluid

Question 16

What microscopic study is the detection of malignancies in the body fluids, usually gives the stages of cancer

Answer 16

Exfoliative cytology

Question 17

What do you call the process of applying dyes on the sections o see and study the architectural pattern of the tissue and physical characteristics of the cells?

Answer 17

Staining

Question 18

What is the most common, cheapest and DIY embedding molds?

Answer 18

Paper boat

Question 19

What is being used to neutralize acid spills?

Answer 19

Soda Ash

Question 20

What method of fresh tissue examination is used in rapid pathologic diagnosis during surgery?

Answer 20

Frozen Section

Question 21

Process of removing intracellular and extracellular water from the tissue

Answer 21

Dehydration

Question 22

What is the simplest, most common used infiltration media?

Answer 22

Paraffin wax

Question 23

Which of the following types is not a microtome?

Answer 23

Biconcave microtome

Question 24

The last step in tissue processing that result in a permanent histological preparation suitable for microscopy?

Answer 24

Mounting

Question 25

Type of infiltration medium recommended for processing of neurological tissues

Answer 25

Celloidin Wax

Question 26

This microscope is useful in examination of unstained microorganism in motion which are too small to be seen by electron microscopy

Answer 26

Darkfield Microscope

Question 27

Anna was asked to prepare a fixative solution for enzyme histochemistry and carbohydrate fixation. While in a rush she unknowingly mislabeled the fixative bottles and switch the fixative solution for enzyme and carbohydrate. After fixation for enzyme histochemistry it was observed that there is a loss and inactivation of enzymes needed for study and for carbohydrate analysis some substances were removed that are significant in the diagnosis. What is most likely the cause of this problem?

Answer 27

Wrong fixative solution used

Question 28

What part of the lens system functions to increase or decrease the magnification?

Answer 28

Objectives

Question 29

What staining process is done when the tissue is first overstained to obliterate the cellular details, and the excess stain is removed or decolorized from unwanted parts of the tissue, until the desired intensity of color is obtained

Answer 29

Regressive Staining

Question 30

A process whereby the clearing agent is completely removed from the tissue and replaced by a medium

Answer 30

Infiltration

Question 31

The following are characteristics of good clearing agent except

Answer 31

It should be dissolve out aniline dyes

Question 32

What type of fixatives are made up of two or more fixatives which have been added together to obtain the optimal combined effect of their individual actions upon the cells and tissue constituents?

Answer 32

Compound Fixative

Question 33

What method of fresh tissue examination is useful in cytological examinations particularly for cancer diagnosis?

Answer 33

Smear Preparation

Question 34

These are slow-acting clearing agents that can be used when double embedding techniques are required

Answer 34

Methyl benzoate and Methyl salicylate

Question 35

Which of the following is the effect of prolonged fixation?

Answer 35

Tissue block are brittle and hard

Question 36

Which instrument is designed to accommodate high sample volumes, and uses rotational agitation and/or microwave heating to obtain fast result

Answer 36

Automatic processor

Question 37

Which of the following is not part of a microtome?

Answer 37

Revolving nosepiece

Question 38

It is known as the malignant cancer of the adipose tissue

Answer 38

Liposarcoma

Question 39

What concentration of the alcohol added to the water to lower surface tension?

Answer 39

95% alcohol

Question 40

Which of the following is not a role of a medical technologist in autopsy?

Answer 40

They establish the cause of death

Question 41

It is used as a temporary ringing agent

Answer 41

Paraffin wax

Question 42

Governs the contrast between cellular details and the background. It is the ratio of the speed of light in a vacuum to the speed of light in the medium.

Answer 42

Refractive Index

Question 43

Type of embedding molds consists of two L-shaped strips of heavy metal arranged in a flat metal plate which can be moved to adjust the size of the mold.

Answer 43

Leukhart's embedding mold

Question 44

What method of fresh tissue examination is used whereby small pieces of tissue are placed in a microscopic slide and is allowed to absorbed a vital stain through capillary action?

Answer 44

Crushing

Question 45

In most instances, dehydration starts by placing the specimen in ___ ethyl alcohol in water

Answer 45

70%

Question 46

Which of the following nomenclature does not belong to benign connective tissue tumors

Answer 46

Adenoma

Question 47

What is the secondary form of fixation whereby a primarily fixed tissue is placed in aqueous solution of 2.5-3% potassium dichromate for 24 hours to act as mordant for better staining effects and aid in cytologic preservation of tissues?

Answer 47

Post-chromatization

Question 48

Which of the following describe CIN3 classification correctly?

Answer 48

Evidence of severe dysplasia in the cervical tumor

Question 49

It is a cheap, rapid-acting dehydrating agent utilized for most urgent biopsies which it dehydrates in 1⁄2 to 2 hours.

Answer 49

Acetone

Question 50

Type of dehydrating agent recommended for routine dehydration of tissues. It is a clear, colorless, flammable fluid.

Answer 50

Ethyl Alcohol

Question 51

It is known as the stiffening of the body

Answer 51

Rigor mortis

Question 52

What is commonly used fixative for proteins

Answer 52

Neutral buffered formalin

Question 53

What is the ratio of egg white to glycerin in Mayer's egg albumin

Answer 53

1:2

Question 54

The _____________ tissue sections are lifted from the knife edge and then floated in a dish of clean distilled water or NSS to maintain cold or at room temperature

Answer 54

Frozen

Question 55

A procedure whereby calcium or lime salts are remove from the tissue

Answer 55

Decalcification

Question 56

This type of clearing agent may be used as a substitute for xylene or benzene for clearing both during embedding and mounting process.

Answer 56

Toluene

Question 57

What do you call the process by which processed tissue, commonly a paraffin embedded tissue is trimmed and cut into uniformly thin slices or “sections” to facilitate studies under the microscope?

Answer 57

Microtomy

Question 58

What is the other term for infiltration

Answer 58

Impregnation

Question 59

Type of infiltration medium recommended for processing of neurological tissue

Answer 59

celloidin wax

Question 60

What grading system tell whether infection or reactive reparative changes are present and gives a general categorization of the specimen?

Answer 60

The Bethesda System

Question 61

What is the desired temperature of a water bath for floatation of tissue sections?

Answer 61

Approximately 6-10 degree Celsius lower than the melting point of the wax used for embedding

Question 62

Which component of the preparedness cycle identifies the skill sets, equipment, technology and other resources needed to execute plans?

Answer 62

Organize

Question 63

What is the substitute infiltration medium which is more elastic and resilient than paraffin wax?

Answer 63

Paraplast

Question 64

What is the purpose of floatation

Answer 64

To flatten the ribbon

Question 65

What is the first step in performing H&E staining

Answer 65

Deparaffinization

Question 66

What is the correct fixative to specimen ratio

Answer 66

20:1

Question 67

The surface of the section to be cut is to be placed parallel to the bottom of the container in which it is cast. This is called:

Answer 67

Tissue orientation

Question 68

A process of sealing the margins of the coverslip to prevent the escape of fluid or semi-fluid mounts and evaporation of mountant.

Answer 68

Ringing

Question 69

Type of embedding mold holding tissue during processing and has a stainless steel lid on the plastic cassette.

Answer 69

Steel Molds

Question 70

What part of the lens system is nearest to the eye

Answer 70

Eyepiece

Question 71

What is a refrigerated apparatus used in fresh tissue microtomy

Answer 71

Cryostat Cold microtome