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Bacte Flashcards

1
Q

What test differentiates staphylococcus against streptococcus and enterococcus?

A

Catalase Test

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2
Q

Pseudomonas aeruginosa can be diagnosed from the pigment known as?

A

Pyocyanin

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3
Q

Which of the following tests is used to detect the alpha-toxin produced by the clostridium perfringes?

A

Nagler Test

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4
Q

Identify a selective medium that allows the isolation of N.gonnorheae and N. meningitides?

A

Modified Thayer-Martin

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5
Q

I. Domain bacteria lack peptidoglycan in cell walls
II. Domain eukarya are usually larger than bacteria and archaea

A

First statement is False, Second statement is True

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6
Q

Based on the picture given above, which of the following statements ring true for letter A?

A

Period of adaptation

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7
Q

All of the following contain short mycolic acid in their cell wall, except?

A

Streptomyces spp.

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8
Q

Gram-negative diplococci recovered from a urethral swab growing on a modified thayer-martin plate and giving a positive oxidase test are presumptively identified as?

A

N.gonorrhoeae

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9
Q

This granule serves as the storage for depot for materials needed by the corynebacterium dipththeriae?

A

All of the above

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10
Q

A group of elementary students became ill after seating undercooked ground beef prepared in the school cafeteria. The suspect pathogen, E.coli serotype 0157:H7, is usually recovered using which of the following media?

A

MacConkey Agar with sorbitol

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11
Q

A medical technologists isolated a clostridium that is non-motile, catalase negative, lecithinase positive with a subterminal endospore. Supposed that you are the medical technologist, what clostridium spp. would you report?

A

Clostridium botulinum

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12
Q

Which of the following are factors that affect the growth of a microorganism
1.Temperature
2.Oxygen
3.pH
4.Salt concentration

A

1,2,3,4

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13
Q

What hemolysis is this?(picture)

A

Double-zone of hemolysis

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14
Q

I. A CAMP test positive result is an arrowhead or bowtie hemolysis
II. CAMP stands for Christine, Atkinson, Munch and Peterson Test

A

First statement is True, Second statement is False

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15
Q

Which of the following serotypes of Listeria monocytogenes causes most of the food born outbreaks?

A

4b

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16
Q

All are intestinal strains of E. coli except?

A

Uropathogenic E. coli

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17
Q

Majority of the pathogenic bacteria thrive in which pH environments?

A

Slightly alkaline environment (7.2-7.6)

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18
Q

Based on motility, which of the following does not belong to the group?

A

Proteus vulgaris

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19
Q

Staphylococcus is catalase___ and coagulase ___

A

Positive, Positive

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20
Q

A specimen was processed in the microbiology laboratory and gram stained smear showed gram negative diplococci. After 24 hrs of incubation on a chocolate agar plate colonies were described as “Hockey-puck” colonies.
What was the possible organism isolated?

A

M. catarrhalis

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21
Q

Which of the following Koch’s postulate?

A

The microorganism must be absent in every case of the disease but present from healthy organisms

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22
Q

What is unacceptable specimen?

A

Barium enema for O&P exam

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23
Q

Mycobacterial disease wherein patient presents
“leonine facie” with presence of skin lesions & symmetric nerve damage due to ineffective cell-mediated immune response?

A

Lepromatous leprosy

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24
Q

When labeling the specimen container, what is usually included?
1. Patient’s Name
2. Complete Address
3. Date/ Time of collection
4. Contact number

A

1& 3

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25
Q

Personnel must use appropriate barrier protection when collecting or handling specimens. What is the correct order of donning PPE?

A

Lab gown, mask, gloves

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26
Q

Actinomycosis is a condition associated with the formation of multiple abscesses and development of sinus tracts discharging white to yellowish granules, known as

A

Sulphur Granules

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27
Q

Which of the following Haemophilus spp require both the X (hemin) and V (NAD) factors for its growth?

A

H. haemolytics

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28
Q

Obligate aerobes will grow only in the presence of oxygen.
Facultative anaerobes are those that grow best in environments where there is presence of low oxygen tension.

A

First statement is True, Second statement is False

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29
Q

The Swan-neck flask experiment was done by whom

A

Louis Pasteur

30
Q

All care catalase positive aerobic gram-positive bacilli

A

Erysipelothrix rhusiopthiae

31
Q

Which of the following is not a charateristic of eukaryotic microorganism

A

Unicellular

32
Q

Which of the following bacterial structures is responsible for the acid-fastness of Mycobacterium spp?

33
Q

Which staining method is employed for the proper examination of Mycobacterium tuberculosis?

A

All of the above

34
Q

If a specimen is to be collected through intact skin, cleanse the site first with what?

35
Q

Vibrio vulnificus and V. parahaemolyticus both gram-negative, motile bacteria are mostly found in warm coastal areas. What is/are the common source through which these bacteria can be transferred to human and cause infections?

A

All of the above

36
Q

Which of the following best describes
fixation?

A

All of the above

37
Q

Which of the following routine culture media allows differentiation of the microorganisms’ different hemolytic patterns?

A

Blood Agar Plate

38
Q

Which component of the GasPak Jar forms water by combining hydrogen and
oxygen inside the jar?

39
Q

Which of the following tubes would contain the MIC of the antibiotic TUBE 1 = 2ug
(Turbid) TUBE 5 = 32ug (Clear) TUBE 2 = 4ug (Turbid) TUBE 6 = 64ug (Clear) TUBE 3 = 8ug (Turbid) TUBE 7 = 128 ug (Clear)
TUBE 4 = 16ug (Turbid)TUBE 8 = 256ug (Clear)

40
Q

Refer to the picture below. What is this
staining technique?

A

Acid Fast Staining

41
Q

How long is the incubation time of your MHA plates in the disk diffusion method?

A

18-24 hours

42
Q

In Eosin-Methylene blue agar, Lactose fermenters appear ___ while non-lactose fermenters appear ___?

A

Violet, clear

43
Q

Among the selection, which is the main purpose of the open flame in the bench top using a bunsen burner?

A

Create a cone of hot air to
reduce the viability of organisms
on suspended dust particles

44
Q

Which organism can cause swarming in MHA plates?

45
Q

Which organism can cause swarming in MHA plates?

46
Q

All of the following are present in gram-positive bacteria, except?

A

Lipopolysaccharide layer

47
Q

Elliot received a water sample in the laboratory with a request for Enzyme-Substrate Technique. He processed the sample immediately by
dissolving the lyophilized reagent substrate in the said water sample and incubated it for 18-24 hours at 350C. The
next day he observed the following results. From the images given below, which of the following would be the correct interpretation of these results?

A

Invalid results and water sample
needs to be retested

48
Q

I.Multiple tube fermentation technique is reported in CFU/mL
II.Enzyme-substrate technique is reported in MPN/100mL

A

1st statement is false, 2nd
statement is true

49
Q

Which of the following is the fluorogenic substrate in the enzyme-substrate technique for total coliform bacteria

A

None of the choices

50
Q

Streak plate method is performed as clock
stoke or ____ streak technique

51
Q

Kristie is preparing a Blood Agar plate. What are the correct steps in accomplishing this? 1-Blood is added 2-
Mix thoroughly the blood and medium by rotating it SIowly 3- Dissolve premixed
media with aid of heat 4- Cool melted blood agar base at 45°C

52
Q

What is the percentage of agar found in a semi-solid medium?

53
Q

Which of the following statements is true
of the aseptic technique

A

All of the above

54
Q

Which of the following organisms does not
have the correct optimum growth temperature?

A

Bacillus anthracis - 42 degrees
C

55
Q

Based on oxygen requirements, how would you classify anaerobes than can still
grown in the presence of oxygen?

A

Facultative aerobes

56
Q

Which of the following is the correct
sequence for preparation of bacterial
smears from solid media? 1. label slides
& add a drop of saline solution 2. pick an
isolated colony and apply smear evenly
3. gently shake tubed media to
re-suspend microorganism 4. air dry &
heat fix smears5. flame inoculating
needle until it turns bright red

57
Q

Which of the following refers to the standard value of multiple tube fermentation technique under tidal coliform
parameter?

A

<1,1 MPN/100/mL

58
Q

Which of the following is not a component of gram staining

A

Methylene blue

59
Q

Which of the following is not used to describe the texture of a bacterial colony?

A

Iridescent

60
Q

Which statement correctly describes basic
dye?

A

It has positively charged groups
that bind to negatively charged
molecules

61
Q

Which of the following statements are
correct I. Crateriform liquefaction would
have a saucer shaped liquid surface II.
Stratiform liquefaction would have
complete liquefaction of the lower half of
the medium III. Arborescent growth would
appear as a tree like growth IV. Beaded
growth would appear like a chain of pears
in consistency

A

Statement I & III are true

62
Q

To avoid moisture from getting into the plates, what must be done prior to incubation?

A

Incubate the plates with the agar
side up

63
Q

What is the standard inoculum size used in kirby bauer method?

64
Q

Which of the following microorganisms
serve as indicators in microbiological
water analysis? 1. Salmonella spp.2.
Escherichia spp.3. Shigella spp. 4. Eikenella spp.

65
Q

Which of the following microorganisms
would grow in Mannitol Salt Agar? 1.
Staphylococcus aureus 2. Streptococcus
pyogenes 3. Staphylococcus epidermidis
4. Neisseria meningitidis

A

1 & 3 only

66
Q

What is the other term for the BSC II

A

Laminar flow biosafety cabinet

67
Q

In preparing a butt media, how far is the depth of the needle inserted into the medium

68
Q

In applying antibiotic disks on the MHA plate, what is the ideal distance of each disk from the edge of the plate?

69
Q

Which of the following organism will
exhibit pink colonies on MacConkey
agar? 1. Escherichia coli 2. Klebsiella
spp. 3. Enterobacter spp. 4.
Staphylococcus aureus

70
Q

What is the concentration of phenol used in Ziehl-Neelsen and Kinyoun’s method,
respectively?

QE REVIEWER (10 decks)

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