Bacte Flashcards
What test differentiates staphylococcus against streptococcus and enterococcus?
Catalase Test
Pseudomonas aeruginosa can be diagnosed from the pigment known as?
Pyocyanin
Which of the following tests is used to detect the alpha-toxin produced by the clostridium perfringes?
Nagler Test
Identify a selective medium that allows the isolation of N.gonnorheae and N. meningitides?
Modified Thayer-Martin
I. Domain bacteria lack peptidoglycan in cell walls
II. Domain eukarya are usually larger than bacteria and archaea
First statement is False, Second statement is True
Based on the picture given above, which of the following statements ring true for letter A?
Period of adaptation
All of the following contain short mycolic acid in their cell wall, except?
Streptomyces spp.
Gram-negative diplococci recovered from a urethral swab growing on a modified thayer-martin plate and giving a positive oxidase test are presumptively identified as?
N.gonorrhoeae
This granule serves as the storage for depot for materials needed by the corynebacterium dipththeriae?
All of the above
A group of elementary students became ill after seating undercooked ground beef prepared in the school cafeteria. The suspect pathogen, E.coli serotype 0157:H7, is usually recovered using which of the following media?
MacConkey Agar with sorbitol
A medical technologists isolated a clostridium that is non-motile, catalase negative, lecithinase positive with a subterminal endospore. Supposed that you are the medical technologist, what clostridium spp. would you report?
Clostridium botulinum
Which of the following are factors that affect the growth of a microorganism
1.Temperature
2.Oxygen
3.pH
4.Salt concentration
1,2,3,4
What hemolysis is this?(picture)
Double-zone of hemolysis
I. A CAMP test positive result is an arrowhead or bowtie hemolysis
II. CAMP stands for Christine, Atkinson, Munch and Peterson Test
First statement is True, Second statement is False
Which of the following serotypes of Listeria monocytogenes causes most of the food born outbreaks?
4b
All are intestinal strains of E. coli except?
Uropathogenic E. coli
Majority of the pathogenic bacteria thrive in which pH environments?
Slightly alkaline environment (7.2-7.6)
Based on motility, which of the following does not belong to the group?
Proteus vulgaris
Staphylococcus is catalase___ and coagulase ___
Positive, Positive
A specimen was processed in the microbiology laboratory and gram stained smear showed gram negative diplococci. After 24 hrs of incubation on a chocolate agar plate colonies were described as “Hockey-puck” colonies.
What was the possible organism isolated?
M. catarrhalis
Which of the following Koch’s postulate?
The microorganism must be absent in every case of the disease but present from healthy organisms
What is unacceptable specimen?
Barium enema for O&P exam
Mycobacterial disease wherein patient presents
“leonine facie” with presence of skin lesions & symmetric nerve damage due to ineffective cell-mediated immune response?
Lepromatous leprosy
When labeling the specimen container, what is usually included?
1. Patient’s Name
2. Complete Address
3. Date/ Time of collection
4. Contact number
1& 3
Personnel must use appropriate barrier protection when collecting or handling specimens. What is the correct order of donning PPE?
Lab gown, mask, gloves
Actinomycosis is a condition associated with the formation of multiple abscesses and development of sinus tracts discharging white to yellowish granules, known as
Sulphur Granules
Which of the following Haemophilus spp require both the X (hemin) and V (NAD) factors for its growth?
H. haemolytics
Obligate aerobes will grow only in the presence of oxygen.
Facultative anaerobes are those that grow best in environments where there is presence of low oxygen tension.
First statement is True, Second statement is False
The Swan-neck flask experiment was done by whom
Louis Pasteur
All care catalase positive aerobic gram-positive bacilli
Erysipelothrix rhusiopthiae
Which of the following is not a charateristic of eukaryotic microorganism
Unicellular
Which of the following bacterial structures is responsible for the acid-fastness of Mycobacterium spp?
Cell wall
Which staining method is employed for the proper examination of Mycobacterium tuberculosis?
All of the above
If a specimen is to be collected through intact skin, cleanse the site first with what?
Either
Vibrio vulnificus and V. parahaemolyticus both gram-negative, motile bacteria are mostly found in warm coastal areas. What is/are the common source through which these bacteria can be transferred to human and cause infections?
All of the above
Which of the following best describes
fixation?
All of the above
Which of the following routine culture media allows differentiation of the microorganisms’ different hemolytic patterns?
Blood Agar Plate
Which component of the GasPak Jar forms water by combining hydrogen and
oxygen inside the jar?
Palladium
Which of the following tubes would contain the MIC of the antibiotic TUBE 1 = 2ug
(Turbid) TUBE 5 = 32ug (Clear) TUBE 2 = 4ug (Turbid) TUBE 6 = 64ug (Clear) TUBE 3 = 8ug (Turbid) TUBE 7 = 128 ug (Clear)
TUBE 4 = 16ug (Turbid)TUBE 8 = 256ug (Clear)
Tube 5
Refer to the picture below. What is this
staining technique?
Acid Fast Staining
How long is the incubation time of your MHA plates in the disk diffusion method?
18-24 hours
In Eosin-Methylene blue agar, Lactose fermenters appear ___ while non-lactose fermenters appear ___?
Violet, clear
Among the selection, which is the main purpose of the open flame in the bench top using a bunsen burner?
Create a cone of hot air to
reduce the viability of organisms
on suspended dust particles
Which organism can cause swarming in MHA plates?
Proteus
Which organism can cause swarming in MHA plates?
Proteus
All of the following are present in gram-positive bacteria, except?
Lipopolysaccharide layer
Elliot received a water sample in the laboratory with a request for Enzyme-Substrate Technique. He processed the sample immediately by
dissolving the lyophilized reagent substrate in the said water sample and incubated it for 18-24 hours at 350C. The
next day he observed the following results. From the images given below, which of the following would be the correct interpretation of these results?
Invalid results and water sample
needs to be retested
I.Multiple tube fermentation technique is reported in CFU/mL
II.Enzyme-substrate technique is reported in MPN/100mL
1st statement is false, 2nd
statement is true
Which of the following is the fluorogenic substrate in the enzyme-substrate technique for total coliform bacteria
None of the choices
Streak plate method is performed as clock
stoke or ____ streak technique
S-streak
Kristie is preparing a Blood Agar plate. What are the correct steps in accomplishing this? 1-Blood is added 2-
Mix thoroughly the blood and medium by rotating it SIowly 3- Dissolve premixed
media with aid of heat 4- Cool melted blood agar base at 45°C
3,4,1,2
What is the percentage of agar found in a semi-solid medium?
0.5%-1.5%
Which of the following statements is true
of the aseptic technique
All of the above
Which of the following organisms does not
have the correct optimum growth temperature?
Bacillus anthracis - 42 degrees
C
Based on oxygen requirements, how would you classify anaerobes than can still
grown in the presence of oxygen?
Facultative aerobes
Which of the following is the correct
sequence for preparation of bacterial
smears from solid media? 1. label slides
& add a drop of saline solution 2. pick an
isolated colony and apply smear evenly
3. gently shake tubed media to
re-suspend microorganism 4. air dry &
heat fix smears5. flame inoculating
needle until it turns bright red
1,5,2,5,4
Which of the following refers to the standard value of multiple tube fermentation technique under tidal coliform
parameter?
<1,1 MPN/100/mL
Which of the following is not a component of gram staining
Methylene blue
Which of the following is not used to describe the texture of a bacterial colony?
Iridescent
Which statement correctly describes basic
dye?
It has positively charged groups
that bind to negatively charged
molecules
Which of the following statements are
correct I. Crateriform liquefaction would
have a saucer shaped liquid surface II.
Stratiform liquefaction would have
complete liquefaction of the lower half of
the medium III. Arborescent growth would
appear as a tree like growth IV. Beaded
growth would appear like a chain of pears
in consistency
Statement I & III are true
To avoid moisture from getting into the plates, what must be done prior to incubation?
Incubate the plates with the agar
side up
What is the standard inoculum size used in kirby bauer method?
1.5
Which of the following microorganisms
serve as indicators in microbiological
water analysis? 1. Salmonella spp.2.
Escherichia spp.3. Shigella spp. 4. Eikenella spp.
1,2,3
Which of the following microorganisms
would grow in Mannitol Salt Agar? 1.
Staphylococcus aureus 2. Streptococcus
pyogenes 3. Staphylococcus epidermidis
4. Neisseria meningitidis
1 & 3 only
What is the other term for the BSC II
Laminar flow biosafety cabinet
In preparing a butt media, how far is the depth of the needle inserted into the medium
3/4
In applying antibiotic disks on the MHA plate, what is the ideal distance of each disk from the edge of the plate?
≥10mm
Which of the following organism will
exhibit pink colonies on MacConkey
agar? 1. Escherichia coli 2. Klebsiella
spp. 3. Enterobacter spp. 4.
Staphylococcus aureus
1,2 and 3
What is the concentration of phenol used in Ziehl-Neelsen and Kinyoun’s method,
respectively?
5% and 9%
