Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Foundation concepts > histology > Flashcards

histology Flashcards

1
Q

Whats the purpose of ECM?

A

Extracellular matrix- transport nutrient, carry away catabolite and support cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are the methods of preparing tissue?

A

1- fixation
2- embedding/sectioning
3- staining
4- microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Whats the purpose of fixation?

A

Avoid tissue digestion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

How does fixation take place?

A
  • Cut tissue
  • immerse tissue in solution with fixative
  • fixative diffuse in it= better tissue preservation
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is an example of fixative?

A

Formalin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Whats the purpose of intravascular perfusion?

A

Better fixation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Whats the purpose of embedding?

A

To section it easily

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Whats the material needed for embedding

A

Parafin (light microscope) and plastic resins (light + electron microscopy)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Whats the process that takes place in embedding/sectioning?

A

Dehydration:
-water + ethanol mixture- takes away water from tissue
-alcohol + embedding substance
Clearing:
-Add tissue in paraffin (60 degrees celcius)
-Add tissue in paraffin in normal temperature

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Whats the purpose of staining?

A

Colorize cell- tissue act as acid + bases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What do DNA react with in staining and whats the color?

A

Base (hematoxylin) reacts with acid (DNA)- color: purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What do collagen react with in staining and whats the color?

A

acid (eosin) reacts with base (collagen)- color: purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Whats point of microscope?

A

Study cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

True or false- light microscope is used for histology

A

False- bright field microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What lens are used in bright field lens?

A

Condenser: Illuminate
Objective: Enlarge
Eyepiece: Magnifies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What type of electron microscopes are there?

A
  • Transmission electron microscope (TEM)

- Scanning electron microscope (SEM)

17
Q

Which EM has 3D view?

A

SEM

18
Q

How to rapidly check biopsies?

A
  • Freeze in liquid nitrogen + use cryosat
  • Microtome sections tissue
  • Microscope
19
Q

How can cryosat be used in another way?

A
  • Study of enzymes

- Clear solvent dissolve lipid in fixed tissue

20
Q

Which are medication application of enzyme histochemistry?

A
  • Perl Prussian blue reaction- check ion
  • Pas- checks amylase- glycogemosis & mucopolysaccharidosis
  • Lipid- check sphingolipidomic
21
Q

What are issues of studying tissues?

A
  • 2D view not 3D
  • Tissue may distort and cause structural abnormalities called artifacts
  • Impossible to show all of tissue
22
Q

True or false- staining can take up to 2 1/2 days

A

True depend on size

Foundation concepts (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions