Histology Flashcards
What is Histopathology?
study of diseased tissues
What is Histology?
Study of normal tissues and structures.
What is Cytology?
Examination of cell structures
4 applications of Histology
Experimental biology
Pathology
Ecotoxicology
Veterinary diagnosis
Histology techniques- 6 stages
1- fixation
2- Dehydration
3- Clearing
4- Decalcification
5- Wax impregnation
6-Blocking, microtomy, staining, and mounting
Gill Pathologies indicators (4)
Mucus secretion
Epithelial swelling
aneurysms
Parasitic infestations
Liver Pathology indicators (4)
Fatty change
Necrosis
Tumour formation
Vascular damage
Reproductive pathology indicators (4
)
Gamete abnormalities
gonad atrophy
tumors
developmental issues in offspring
Histology Case study
Sticklebacks used to study biomarker responses to pollution (e.g., gill and liver histology)
Biomarkers in Environ
Histological Biomarkers in Environmental Monitoring:
A measurable biological response to contaminants.
Assesses exposure and effects using biochemical, physiological, and histological methods.
Challenges in pollution monitoring
Variability due to abiotic and biotic factors (e.g., water quality, temperature, pathogens).
Requires robust experimental design and normalization against factors like size and nutrition.