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HEMATOLOGY 1 > HEMOGLOBINOMETRY > Flashcards

HEMOGLOBINOMETRY Flashcards

1
Q

most common anticoagulant for routine hematology testing

A

ethyldiaminetetraacitic acid (edta)

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2
Q

what tests has minimal effects on blood cell morphology

A

complete blood count
differential count

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3
Q

how long does CBC testing should be stored if it is stored at RT and if it is stored at 4’C

A

6hrs - RT
24hrs - 4’C

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4
Q

why peripheral blood films should be prepared within 3hrs?

A

reduce cell detoriation and morphology artifacts

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5
Q

may be necessary to use manual methods when counts exceed the linearity of an instrument

A

manual cell count

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5
Q

equipment used in manual cell count

A

hemacytometer

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6
Q

purpose of platelet count

A

to count the platelets in a 1 liter of blood

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6
Q

lyses the nonnucleated rbcs in the specimen to prevent interference in the count

A

diluting fluid

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6
Q

purpose of wbc count

A

to count the wbc’s in a 1 liter of blood

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6
Q

diluting fluid used in wbc count

A

1% buffered ammonium oxalate

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7
Q

ratio of platelet to diluting fluid

A

1:100

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7
Q

they are small and can be confused easily with dirt or debris

A

platelet

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7
Q

ratio of wbc to diluting fluid

A

1:20

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7
Q

diluting fluid used in platelet count

A

1% ammonium oxalate

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8
Q
A
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8
Q

the adherence of platelets found around neutrophils, producing a ring or satellite effect. what anticoagulant is used in this phenomenon.

A

platelet satellitosis; edta

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9
Q

what anticoagulant should correct the platelet satellitosis

A

sodium citrate

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10
Q

more accurate manual rbc procedures

A

hematocrit
hemoglobin concentration

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10
Q

rarely performed because of the innacuracy of the count and questionable necessity

A

red blood cell count

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11
Q

commercially available for wbc and platelet counts

A

capillary pipette
diluent reservoir systems

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11
Q

carry oxygen to and carbon dioxide to the tissues

A

hemoglobin

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12
Q

it consists of a capillary pipette that fits into a plastic reservoir containing 1.98% ml of 1% buffered ammonium oxalate

A

Leuko Chek

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13
Q

what is the absorbance of cyanmethemoglobin and is it direct or indirectly proportional to the hemoglobin concentration

A

540nm; directly proportional

14
Q

this reagent is sensitive to light and it should be stored in a brown bottle

A

cyanmethemoglobin reagent

15
Q

what ingredient of the hemoglobin reagent is highly toxic and must be used cautiously

A

cyanide

16
Q

volume of packed rbcs that occupies a given volume of whole blood

A

hematocrit

17
Q

this instrument uses precalibrated capillary tubes and has built-in hematocrit scales, which eliminates the need for separate reading devices

A

READACRIT centrifuge

18
Q

This rule applies only to specimens that have normcytic normochromic rbcs

A

rule of three

18
Q

what are the redd blood cell indices

A

mean cell volume
mean cell hemoglobin
mean cell hemoglobin concentration

19
Q

eliminates the use of sealants

A

SUREPREP capillary tubes

20
Q

average volume of rbcs expressed in femtoliters

A

mean cell volume

20
Q

average weight of hemoglobin in a RBC, expressed in picograms

A

mean cell hemoglobin

21
Q

average concentration in each individual rbc. the units used are grams per deciliter

A

mean cell hemoglobin concentration

22
Q

last immature rbc stage

A

reticulocyte

22
Q

precipitated hemoglobin, usually appear round or oval, and tend to adhere to the cell membrane

A

heinz bodies

23
Q

round nuclear fragments and usually singular

A

howell-jolly bodies

23
Q

iron in the mitochondria whose presence can be confirmed with an iron stain, such as prussian blue

A

pappenheimer bodies

23
Q

reticulocytes that are released from the marrow prematurely

A

shift reticulocytes

24
Q

useful in detecting early hematopoietic activity after chemotheraphy or hematopoietic stem cell transplantation

A

Immature Reticulocyte Fraction (IRF)

25
Q

useful to detect early iron deficiency

A

reticulocyte hemoglobin

25
Q

tests to detect and monitor the course of inflammatory conditions such as rheumatid arthritis, infections,or certain malignancies

A

erythrocyte sedimentation rate

26
Q

not a specific test for inflammatory diseases and is elevated in many other conditions also it is not recommended as a screening test to detect inflammatory conditions in asymptomatic individuals

A

erythrocyte sedimentation rate

27
Q

most commonly used method today that the taller column height allows the detection of highly elevated esrs

A

modified westergren erythrocyte sedimentation rate

28
Q

offers the ability to produce rapid and accurate results that facilitate faster treatment, which can decrease hospital length of stay

A

point -of-care testing

28
Q

this method uses oxalate-anticoagulated whole blood

A

wintrobe erythrocyte sedimentation rate

29
Q

means that regardless of where the diagnostic testing is performed or who performs the test, all testing sites must follow the same regulatory requirements based on the complexity of the test

A

testing site neutrality

30
Q

useful in selecting equipment that best covers the patient population for the institution

A

patient correlation studies

31
Q

use the conductivity method to determine the hematocrit

A

i-STAT 1 and Epoc

32
Q

instrument that eliminates the interference from turbidity in the specimen and what is its waveleghths

A

HemoCue hemoglobinometer; 570nm and 880nm

33
Q

used to detect indirectly evaluate the oxygen carrying capacity of the blood

A

hemoglobin estimation

34
Q

physiological factors in hemoglobin estimation

A

age
gender
pregnancy
physical activity or exercise

35
Q

pathological factors in hemoglobin estimation

A

testosterone deficiency
renal deficiency
myelodysplasia
diminished erythrocyte
bone marrow suppresion
blood loss

36
Q

most accurate method for estimation of hb

A

cyanmethemoglobin method

37
Q

specimens used in cyanmethemoglobin method

A

edta anticoagulated blood
capillary blood

38
Q

this method is based on the capacity of hemoglobin to combine with the same maximum volume of carbon dioxide as of oxygen

A

gasometric method

39
Q

this method or technique of measuring hemoglobin is not routinely performed except as a method of screening blood donors

A

gravimetric method

40
Q

hydrochloric acid and hemoglobin produces what and what is its color

A

acid hematin; brown hematin

HEMATOLOGY 1 (6 decks)

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