HEMATOPOIETIC SYSTEM Flashcards
During the normal maturation of RBC or cell, what would be the changes that will occur?
- size
- nucleus
- cytoplasm
- chromatin
- granules
the cytoplasm of an immature
cell is usually blue or basophilic due to RNA content.
Loss of Basophilia
These granules contain some
enzymes which distinguish the myeloid stem cells from other cells
cytoplasmic granules
acidophilic or eosinophilic granules
red dye
are called basophilic
granules
blue dye
basic and acidic dye
Neutrophilic granules
special feature
of the maturation of erythrocytes cells.
Elaboration of Hemoglobin
How many series are there in the maturation of erythrocytes?
6 series
the nucleus is apparently no
longer necessary and eliminated by
nucleolysis or extursion
Mature stage
anucleated cells
erythrocytes
The immature nucleus is round or oval and the nuclear chromatin is very delicate, fine and linear and is called
EUCHROMATIN
it would give a pale blue color, uncondensed
EUCHROMATIN
As the cells matures chromatin strands
increasingly coarse and clumped
HETEROCHROMATIN
in terms of size, a mature cell is _______
than immature cells.
smaller
Enumerate the nuclear changes
- Structure and Cytochemistry
- Shape Changes
- Reduction in cell size
Inclusion bodies may be found
in the cytoplasm of both erythrocytes and
leukocytes, especially in the granulocytes
abnormal cytoplasmic
this is characterized by persistent
cytoplasmic basophilia and late
hemoglobinization.
Abnormal Cytoplasmic Differentiation
Does immature cells contain hemoglobin?
No
best example for mature stage
erythrocyte
What do you call if the lobes of neutrophil is 6-10?
Hypersegmented neutrophils
What could be the reason why neutrophils has many lobes?
- old cell
- megaloblastic anemia
What are the example of inclusion bodies that are present in RBC
- Howell-Jolly bodies,
- Pappernheimer bodies
- Heinz bodies
Hyposegmented is seen in condition
associated with
Pelger-Huet Anomaly ; problem with lamin B
less than two lobes
Hyposegmented neutrophils
types of human stem cell
- Totipotential stem cells
- Pluripotential stem cells
- Multipotential stem cells
These cells are present several days after
fertilization. can develop into any cell type, except they cannot develop into a FETUS.
Pluripotential stem cells
These cells are present in the first few
hours after an ovum is fertilized. most versatile type of stem cell, can develop into any human cell type, including development from embryo into
fetus
Totipotential stem cells
These cells are derived from pluripotent
stem cells. They can be found in adults, but they are limited to specific types of cells to form tissues
Multipotential stem cells
reticulocyte can stay in the peripheral blood for how long?
1 day
reticulocyte can stay in the bone marrow for how long?
1-2 days
the first recognizable
erythroid precursor
rubriblast
Granulopoiesis - they only have one way; they only differ in the matured stage
Basophils
Eosinophils
Neutrophils
is a process by which erythroid
precursor cells differentiates to become mature
Erythropoiesis
Other name for Pronormoblast
Rubriblast
N:C ratio of Pronormoblast
8:1
Other name for Basophilic Normoblast
Prorubricyte
N:C ratio decreases from 8:1 to 6:1
Basophilic Normoblast
globin production begins
Rubricyte
begins to accumulate the
components necessary for hemoglobin
production.
Pronormoblast
Detectable hemoglobin synthesis occurs, but the many cytoplasmic organelles, including ribosomes and a substantial amount of messenger ribonucleic acid
Basophilic Normoblast
Late Normoblast
Metarubricyte
LAST STAGE OF MITOSIS
Rubricyte
The increase in the salmon-pink color of the cytoplasm reflects nearly complete
hemoglobin production
Metarubricyte
First stage of maturation
Prorubricyte
presence of hof
neutrophils Promyelocytes
No nucleus but has mitochondria and
ribosomes
reticulocyte
Last stage to synthesize hemoglobin
reticulocyte
reticulocyte also known as
Polychromatophilic erythrocytes
Polychromatophilic macrocytes
Diffusely basophilic erythrocytes
salmon pink-staining cell with a central
pale area.
erythrocytes
Make up 0% to 3% of the nucleated cells in
the bone marrow and measure 14 to 20 mm in diameter
Myeloblasts
Nucleoli are absent
Neutrophil metamyelocytes
can be identified
cytochemically due to the presence of Charcot
Leyden crystal protein in their primary granules
Eosinophilic promyelocytes
eosinophils have a circulating half-life of
roughly
18 hours
cytoplasm is colorless and contains
large numbers of the characteristic large
blue-black granules
mature basophils
have round to lobulated nuclei
with only slightly condensed chromatin.
immature basophils
contain a lobulated nucleus that is
often obscured by its granules
mature basophils
moderately basophilic to blue or
gray; non granular
monoblast
Cleavage occurs
Promonocytes
may have
small blunt pseudopods, narrow band around the
nucleus; as the cell matures the amount of
cytoplasm increases
Megakaryoblast (STAGE 1)
more abundant than in previous
stage; granules begin to form in the golgi region
Promegarkayocytes (Stage II)
contains
coarse clumps of granules
aggregating into little
bundles which bud off from
the periphery –plts
Megakaryocyte (Stage III)
cytoplasm is light blue – purple
platelets
this stage lasts more than 24 hours
rubriblast
no nucleoli are present
rubricyte or normoblast
first stage in color changing
rubricyte
the stage in rubricyte lasts for
30 hours
nucleus is completely condensed
orthrochromic normoblast or metarubricyte
not capable of division due to the condensation of the chromatin
metarubricyte
last stage in bone marrow before release to the blood
reticulocyte
stain living cells
supravital stain
earliest recognizable granulocyte precursor
myeloblast
also known as juvenile cell
neutrophil metamyelocyte
it is water soluble and may be dissolve if the blood film is washed too much during the staining process
immature basophils
cleavage occurs
promonocytes
lacelike or stringy
promonocytes
would go to the sites of injury to temporarily prevent the bleeding
platelets
