Hematopoietic Stem Cell Transplant

Question 1

only established therapeutic application of stem cells

Answer 1

HSCT
- BM
- peripheral blood
- umbilical cord blood HSC transplant

NOTHING CONTROVERSIAL HERE

Question 2

how do they collect PB HSC transplant source?

Answer 2

gives patient mobilizing agents to push maturation and aspiration of immature stems cells into peripheral blood = monitored and when peaks = collect stem cells based on density

Question 3

T or F. somatic stem cells are pluripotent

Answer 3

F! dedicated to lineage of HSC and heme terminal cells (leukocytes, erythrocytes, etc.)
- more differentiated than embryonic cells
- limited ability to self-renew
- NOT CONTROVERSIAL

Question 4

rational for HSCTs

Answer 4

reconstituting BM and restoring normal hematopoiesis after myeloablative chemotherapy

• hematologic malignancies
• nonmalignant acquired BM disorders = aplastic anemia
• genetic diseases associated with abnormal hematopoiesis
• solid tumors chemotherapy
• definitive or adjuvant therapy

Question 5

characteristics of hematopoietic stem cells

Answer 5

CD34 = main
CD133
Lin
CD117
BCRP

Question 6

what do we use to determine cells that have been harvested for HSCT

Answer 6

flow cytometry

Question 7

HSCT graft sources

Answer 7

syngeneic
allogeneic = siblings, MUD
autologous

Question 8

the autologous transplant process

Answer 8

1. collection = stem cells collected from pt’s BM or blood
2. processing = blood or BM processed in lab to purify and concentrate stem cells
3. cryopreservation (stored for ~3-4 wks until pt is well enough to be able to receive their own stem cells)
4. chemotherapy
5. reinfusion = thawed stem cells back into pt

Question 9

T or F. Cancer cells withstand cryopreservation

Answer 9

F! they do not but stem cells can as they are hearty

Question 10

HSC processing

Answer 10

• weigh
• volume reduced