Hematology Slideset Two Flashcards
1) Rubriblast
2) Prorubricyte
3) Rubricyte
4) Metarubricyte – nucleated RBC
5) Basophilic erythrocyte with Howell Jolly body
6) Basophilic erythrocyte
7) Mature erythrocyte
1) Myeloblast w/a nucleolus
2) Progranulocyte
3) Neutrophilic myelocyte
4) Neutrophilic myelocyte
5) Neutrophilic band cell
6) Monolobed neutrophil – early seg
7) Segmented neutrophil
8) Eosinophilic myelocyte
9) Eosinophilic metamyelocyte
10) Eosinophilic band cell
11) Eosinophil
12) Basophilic myelocyte
13) Basophilic metamyelocyte
14) Basophil
Normal dog leukocytes
Neutrophilic band
Neutrophilic metamyelocyte
Monocyte with vacuoles
Eosinophil with granules of varying sizes, nucleus often unsegmented
Large lymphocyte
Small lymphocyte
Neutrophil and basophil: note small nuclear thrombocytes in lower right
Basophil with subtle lavender granules
Basophil and Monolobed neutrophil
Large clump of thrombocytes
Eosinophil with rod-shaped granules
Neutrophil and monocyte
Lymphocyte
Lymphocyte with large nucleus
Eosinophil with large round granules (horse)
Eosinophil and large lymphocyte (horse)
Basophil and few delicate thrombocytes (horse)
Monocyte (horse)
Small lymphocytes
Neutrophil and small lymphocyte
Bone marrow comparing small lymphocytes (lower) with 2 metarubricytes immediately above lymphocyte. The uppermost cell is a lymphocyte, followed counter clockwise by a band neutrophil and free reticulum cell nucleus.
A metarubricyte (lower right) is touching a small lymphocyte.
Monocyte with blue-gray cytoplasm but without vacuoles.
A monocyte (center cell) surrounded on either side by metamyelocyte. Cytoplasm of metamyelocyte is more red than a monocyte.
Lymphocyte with slightly compressed nucleus and cytoplasm. Erythrocyte abnormalities.
Crenated erythrocytes, margins appearing as sharp points. May result when blood slides dry slowly or as a normal process in aging of red cells.
Basophilic stippling, blue staining granules in central erythrocyte and poikilocytes. In the dog, the presence of basophilic stippled erythrocytes and enucleated erythrocytes, in the absence of severe anemia, suggest a diagnosis of lead toxicosis.
Poikilocytes, irregularly shaped erythrocytes. May be noticed in blood of anemic animals and indicate either improper production or premature destruction of erythrocytes.
Rouleau (plural rouleax), erythrocytes in a roll or column. Rouleau is most pronounced in horse blood.
Anisocytosis – variation in erythrocyte size; when accompanied by polychromasia erythropoiesis indicated.
Acanthocytes, erythrocytes with blunt projections, thought to be a membrane defect; commonly noted in kidney disease, splenic disease (hemangiosarcoma) auto-immune hemolytic anemia and occasionally in liver disease.
Spherocytes, dense spherical canine erythrocytes lacking normal biconcave shape. Polychromasia, large blue erythrocytes. Spherocytes indicate red cell membrane abnormality associated with an increased permeability to sodium ions and is associated with autoimmune mechanism hemolysis. Polychromasia indicated evidence of erythropoiesis.
Reticulocytes – blue reticulum stained with new methylene blue and counterstained with Wright’s stain. Reticulocytes are the best indicator of regenerative anemia in peripheral blood.
Erythrocytic inclusion bodies, a rare finding in Canine Distemper. These inclusions are generally larger than Howell-Jolly bodies and stain paler blue.
Erythrocytic inclusion bodies, a rare finding in Canine Distemper. These inclusions are generally larger than Howell-Jolly bodies and stain paler blue.
Rectangular erythrocyte in dog blood, rarely noted and significance unknown.
Erythrocyte inclusions again, except numerous inclusions in one erythrocyte.
Target cells. Irregularly shaped erythrocytes with the appearance of a target. Target cells are observed along with polychromasia in regenerative anemia. However, their appearance without polychromasia suggest the possibility of kidney, liver or splenic diseases.
Heinz bodies, refractile inclusions of denatured hemoglobin in the cat. These inclusions often protrude causing bulging erythrocyte. The oxidant drug, new methylene blue, produced these changes in the cat’s erythrocytes.
Heinz bodies in cat, same as previous slide except slide stained with new methylene blue.
Heinz bodies and reticulocytes, stained with new methylene blue.
Heinz bodies in horse caused by phenothiazine toxicity. Stained with new methylene blue. Phenothiazine is an old type dewormer still used occasionally as an additive for horse and cow dewormers.
Same as previous slide but not stained with new methylene blue.
Red blood cells – slight polychromasia, and anisocytosis. Note the occasional target cell.
Dog bone marrow erythrogenesis.
RBC’s – marked anisocytosis.
RBC’s – marked hypochromasia with slight polychromasia and anisocytosis. Note the occasional target cell.
Demonstrating increased number of erythrocytes with a blue reticulum, reticulocytes. Stained with new methylene blue and counterstained with Wright’s stain.
RBC’s moderate anisocytosis and polychromasia with numerous Howell-Jolly bodies and metarubricytes.
Many erythrocytes with abnormal projections, often thorn-like, are called acanthocytes. X1000.
RBC’s – numerous target cells.
Dog with Leukocytosis and Non-regenerative anemia in Myocarditis. RBC’s are morphologically normal. Leukocytes – showing a leukocytosis with a neutrophilia, a slight left shift and a monocytosis. Thrombocytes were determined to be normal by finding numerous clumps at the feather edge.
Same slide as previous one. Note – no polychromasia but a rubricyte and a likely monocyte on the right.
Dog with Regenerative left shift in peritonitis. RBC’s appear normal. Leukocytes slightly increased in numbers, neutrophilia with increased number of bands, cytoplasm of neutrophils is noticeable basophilic. Thrombocytes normal, clumped at feather edge. X320
RBC are crenated; neutrophilic band with marked basophilia of cytoplasm. X1000
Cat with degenerative left shift in pyothorax. RBC normal. Leukocytes are within normal range, yet many neutrophilic bands with basophilic cytoplasm. Note the 3 bands.
Cat-leukopenia associated with subcutaneous cellulites. RBC normal. Leukocytes marked decrease in numbers, neutrophilic bands were increased and contained basophilic cytoplasm.
