HEMA W2:5 Flashcards
Qualitative assessment
- Performed to assess function of
platelets and clotting factors in
response to injury by forming a stable
clot, this also includes the test for
regulatory proteins that balances
bleeding and clotting
Coagulation assays
focuses on
vascular integrity and platelet response
Primary hemostasis
focuses on
coagulation proteins in plasma
Secondary hemostasis
focuses on the lysis of
formed clot as healing occurs
Fibrinolysis
- Bone marrow examination – bone cancer
- Cytochemical stains – severe anemia
- Flow cytometry tests
- Molecular diagnostics
Advanced hematological procedures
(Severe Cases)
- G-6-PD assay
- Sickle cell solubility assay
- Hemoglobin electrophoresis
- Erythrocyte sedimentation rate
- Osmotic fragility tests
Special hematological procedures
implies the ability to provide accurate,
reproducible assay results that offer clinically
useful information
Quality
selection on
patient, tests to perform, specimen
collection and handling
Pre-analytical variables
competence of
technician, selection of instrument
Analytical variables
filling of results,
therapeutic ranges, turnaround time, cost
analysis
Post analytical variables
Quality assurance encompasses the following
parameters to assess
Pre, analytical and post analytical
the measure between an
assay value and the theoretical thru value of its analyte.
Accuracy
o Analysis of variance of more than two sets
o Pearson Correlation Coefficient
o Linear Regression
o Bland-Altman Difference Plot
Accuracy
simple validation effort as it is compared
between other test samples to determine the
possibility of errors
Precision
o Standard deviation
o Coefficient in variables
Precision
