HEMA W2:3 Flashcards
Main component of red cells, giving off a
salmon pink color
HEMOGLOBIN
Responsible for the ability of red cells to
transport and release gases
HEMOGLOBIN
Determined by the presence of central pallor
resulting to its biconcave shape.
HEMOGLOBIN
diagnosis used for the red cells
loss of O2 carrying capacity
Anemia
used to note in the increase of
circulating red cell production
Polycythemia
- Determines the number of red cells in per
microliter or per liter of blood - Used for differential diagnosis of anemia and
polycythemia
RBC count
RBC count methods
o Manual counting
o Automated analyzers
Determines the hemoglobin concentration, a
parameter to assess a case of anemia
Hemoglobin Determination
gold
standard for determination
Cyanmethemoglobin method
Relies on the reaction after the addition of
Drabkin’s reagent component:
Hemoglobin Determination
Drabkin’s reagent component:
o Potassium cyanide
o Potassium ferricyanide
o Sodium carbonate or Dihydrogen potassium
phosphate
o Surfactant or Detergent
Referred as the packed cell volume (PCV),
based on the ratio of packed red cells volume
and whole blood volume; reported as
percentage or liter per lite
Hematocrit Determination
Methods for Hematocrit determination
(1) microhematocrit method (2)
macrohematocrit method
- Requires three numerical results: RBC
count, Hemoglobin and Hematocrit count - Measures parameters of a SINGLE red
cells for the assessment of anemia
➢ RBC Indices
Mean cell/corpuscular volume (MCV
size
Mean cell/corpuscular hemoglobin (MCH)
o Hemoglobin
o Pallor
o Color
Mean cell/corpuscular hemoglobin
concentration (MCHC)
o Concentration
o Pallor
o Color
Amount of normal/abnormal cells
Red cell distribution width (RDW)
- Requires Wright-stained peripheral blood
smear at 500-1000x total magnification - Assesses red cells based on diameter,
color, shape, and presence of inclusions
RBC Morphology
Immature form of RBC/young version
- Also known as the polychromatic or
polychromatophilic erythrocytes; contains RNA
remnants
RETICULOCYTES
Newly released cells from bone marrow and
subject to maturation becoming erythrocytes
o Matures in the blood vessels
o Reticulocytes can only mature in the
bloodstream.
o Exits in the peripheral blood
RETICULOCYTES
Assesses the ability of bone marrow to increase
RBC production in response to anemia
o Hypoxia is low levels of oxygen in your
body tissues.
RETICULOCYTES
low levels of oxygen in your
body tissues
Hypoxia
Observed by Supravital stained peripheral
blood that can stain cells in living stat
RETICULOCYTES
Supravital stains
o New methylene blue
o Brilliant cresyl blue
o Crystal violet
Mainly responsible for the protection and
provides immunity against infection and/or
injury
. LEUKOCYTES
Either transported from bone marrow or
lymphoid tissue in response to infection
. LEUKOCYTES
Granuloyctes
Neutrophils, Eosinophils,
Basophils
Aggrunolocytes
Monocytes, Lymphocytes
