HC6- DSB repair visualization Flashcards
1
Q
size DNA double helix
A
2 nm
2
Q
nucleosome core
A
8 histon molecules > tetramer of 2 H3 and 2 H4 with 2 H2A/H2B dimers
3
Q
Histone H1
A
linker molecule
4
Q
size nucleosome core
A
11 nm
5
Q
size beads on a string
A
30 nm > 300 nm
6
Q
fiber width
A
250 nm
7
Q
chromosome size
A
1400 nm
8
Q
DSBs caused by
A
- ionizing radiation
- UV light
- metabolic processes
9
Q
foci are formed by
A
most proteins involved in DNA repair
10
Q
DNA repair proteins upon radiation
A
relocate to sites of DNA damage
11
Q
DNA repair proteins
A
RNF168, 53BP1, MDC1
12
Q
GFP
A
for visualization and quantification of DNA repair proteins
13
Q
diffusion of DNA repair proteins
A
get to DSBs by chance
14
Q
laser microirradiation (quantifying accumulations)
A
readout for repair activities
15
Q
drawbacks laser microirradiation
A
- dosage difficult to establish and control
- mostly UV-type DNA lesions
- large dosage concentrated in small area of the nucleus
