Harnessing Biology Flashcards

1
Q

What is yeast used for in making wine

A

Ferment sugars in grape juice

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2
Q

Why are vats used in making wine

A

Prevent air from reaching wine, ensuring conditions are anaerobic

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3
Q

How are the yeast cells killed in making wine

A

Alcohol conc. increases until it killls yeast cells, fermentation stops

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4
Q

What is beer made of

A

Barley

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5
Q

What are small scale fermenters fitted with?
What does this allow?
(Wine)

A

Airlock
- allows CO2 to escape
- prevents entry of oxygen

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6
Q

What does the fungus penicillium produce?

A

The antibiotic penicillin

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7
Q

What is the function of the cooling jacket in an industrial fermenter

A

Removes heat energy
-> stopping the fermenter overheating and enzymes denaturing

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8
Q

What is the function of air filter in an industrial fermenter

A

Filters air coming in
-> maintaining sterile conditions

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9
Q

What is the function of stirrers in an industrial fermenter

A

Keep broth well stirred
-> oxygenate all parts of the fermenter
-> prevent micro organisms settling

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10
Q

Function of the growth medium in an industrial fermenter

A

Contains all necessary glucose and amino acids
-> micro organism growth

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11
Q

Function of super heated steam in industrial fermenter

A

Applied before fermenter is used
-> kills unwanted micro organisms
> leaves only water + no other residue

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12
Q

What bacteria is yogurt made with

A

Lactobacillus bulgaricus

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13
Q

What happens to milk to kill bacteria (yoghurt)

A

Pasteurised (heated)

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14
Q

What is added to the milk after it cools?
What temp is it maintained at, at this stage?
(Yoghurt)

A

Lactobacillus
46 degrees C

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15
Q

What does maintaining the mixture at 46 degrees C ensure?
(Yoghurt)

A

The bacteria’s enzyme aren’t denatured

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16
Q

Process of making yoghurt?

A
  • equipment is sterilised
  • milk is pasteurised (heating)
  • lactobacillus is to cooled milk + left at 46 degrees C for 4 hours
  • lactobacillus anaerobically respires -> lactic acid
  • further cooled to 5 degrees C
17
Q

What does lactobacillus produce when anaerobically respiring?

A

Lactic acid

18
Q

lactobacillus respires anaerobically to produce lactic acid. What does this do

A
  • Lowers the pH, acting as a preservative
  • acid coagulates the milk protein
    -> as the acidic conditions change the shape of the proteins - giving yoghurt flavour and texture)
19
Q

What is anaerobic respiration in yeast used for making?

A

Beer brewing
Bread making

20
Q

Anaerobic equation in yeast?

A

Glucose -> ethanol + carbon dioxide
C6 H12 O6 -> 2C2 H5 OH + 2CO2

21
Q

What is ethanol and where is it found

A

Alcohol found in alcoholic drinks (beer and wine)

22
Q

How does bread rise

A

Bubbles of carbon dioxide gas expand the dough

23
Q

Does ethanol remain after the bread has been cooked

A

No
It has evaporated due to the high temps

24
Q

2 ways to detect the presence of carbon dioxide

A

Limewater - turns cloudy white
Hydrogen carbonate - orange -> yellow

25
Method for detecting carbon dioxide using a delivery tube
- dissolve sugar in boiled water - add yeast + mix + pour into boiling tube - add layer of vegetable oil - connect delivery tube to second boiling tube - containing limewater or hydrogen carbonate indicator
26
What does the layer of vegetable oil do (when detecting carbon dioxide)
-Prevents oxygen entering - allows carbon dioxide to escape - ensures anaerobic respiration is taking place
27
What are the conditions of the carbon dioxide experiment (delivery tube) that can be investigated?
- temperature - conc. of sugar - type of sugar
28
Definition of fermentation
Any metabolic reaction ( aerobic or anaerobic) by microorganisms to make a useful product
29
What does barley contain
Starch - needs to be broken down
30
When barley seeds germinate, what do they produce?
Amylase
31
What does amylase break down when making beer?
Amylase breaks Starch -> maltose Maltose breaks maltose -> glucose
32
How is glucose fermented? (Beer)
By yeast in a large open vat
33
What is added together to make bread?
Wheat, flour, water, yeast
34
What does yeast allow to occur (bread) when there is air
Aerobic respiration
35
What does enzymes break down in bread?
Starch -> sugar (Sugar is then respired by yeast)