Haemophilus

Question 1

2 species of Haemophilus

Answer 1

Haemophilus influenza

Haemophilus ducreyi

Question 2

morphology of Haemophilus?

Answer 2

Gram negative coccobacilli
Encapsulated strains- Hib
unencapsulated stain- nontypeabe

Question 3

growth factors for H. influenza?

Answer 3

X factor (hemin), V factor (NAD)
chocolate agar plate

Question 4

Disease of H. influenza (unencapsulated)

Answer 4

respiratory infections
otitis media
sinusitis
bronchitis
pneumonia
COPD

Question 5

Disease of Hib (encapsulated)? susceptible age group

Answer 5

Invasive disease:
Meningitis in children
septic arthritis
baceteremia
cellulitis 
epiglottitis
conjuctivitis
susceptible ages to invasive disease: 6mo-3 years

Question 6

Virulence factors

Answer 6

Polysaccharide capsule- most important
hemagglutinating pili
LOS
biofilm formation

Question 7

What is the capsule of Hib?

Answer 7

polyribosyl ribitol phosphate (PRP)

Question 8

How is Hib diagnosed?

Answer 8

Culture (chocolate agar with X and V factor)

latex particle agglutination test

Question 9

H. influenza treatment?

Answer 9

unencapsulated (respiratory) infection: Amoxicillin (+ clavulonic acid)

Encapsulated infections (invasive): 3rd generation cephalosporins (cefotaxime, ceftriaxone, ceftazidime)

Question 10

H. influenza prevention

Answer 10

Vaccine- Hib capsule polysaccharide (PRP) conjugated with protein carrier