GRAM POSITIVE COCCI.2 Flashcards

1
Q

BACITRACIN
1. _____ vs ______
2. ZOI mm

RESULTS:
(+)
(-)

A
  1. group A vs group B
  2. 10mm

RESULTS:
(+) Streptococcus pyogenes (Group A)
(-) Streptococcus agalactiae (Group B)

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2
Q

PYR
- aka

  1. ____ vs _____
  2. enzyme:
  3. enzyme hydrolyzes _______
  4. to produce _______
  5. reagent
  6. positive results

RESULTS:
(+)
(-)

A

PYR
- I-PYRROLIDONYL ARYLAMIDASE TEST

  1. group A & entero vs group B
  2. l-pyrrolidonyl arylamidase
  3. l-pyrrolidonyl-beta-naphthylamide
  4. beta-naphthylamine
  5. N,N-methylamino-cinnamaldehyde reagent
  6. bright red color within 5mins
  7. no color change or an orange color

RESULTS:
(+) Streptococcus pyogenes (Group A) & Enterococcus
(-) Streptococcus agalactiae (Group B)

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3
Q

CAMP
1. aka
2. ___ vs _____
3. produces _______
4. the product acts synergistically w/ ______
- to cause enhanced lysis of ___
5. positive results
6. negative results

RESULTS:
(+)
(-)

A

CAMP
1. CHRISTIE, ATKINS, MUNCH-PETERSON (CAMP) TEST
2. group A vs group B
3. diffusible extracellular hemolytic protein (CAMP,factor)
4. beta-lysin of S. aureus
- RBCs
5. enhanced hemolysis is indicated by arrowhead-shaped zone
6. no enhancement of hemolysis

RESULTS:
(+) Streptococcus agalactiae (Group B)
(-) Streptococcus pyogenes (Group A)

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4
Q

HIPPURATE HYDROLYSIS
1. ____ vs _____
2. end products of hydrolysis of hippuric acid by ____
- includes (2)
3. ____ is deaminated by _____ (oxidizing agent)
4. medium must contain only ______
- as ninhydrin might react w/ any _____ present
5. positive result
6. negative result

RESULTS:
(+)
(-)

A
  1. group A vs group B
  2. hippuricase
    - glycine & benzoic acid
  3. glycine
  4. hippurate
    - free amino acids
  5. deep purple color
  6. colorless or slightly yellow pink color

RESULTS:
(+) Streptococcus agalactiae (Group B)
(-) Streptococcus pyogenes (Group A)

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5
Q

OPTOCHIN
______ vs _____
1. aka
2. optochin (______)
3. interferes with ____
4. production of
5. ZOI mm

RESULTS:
(+)
(-)

A

s.pneumoniae (presumptive identification) vs other alpha (viridans)
1. TAXO P
2. ethylhydrocupreine hydrochloride
3. ATPase
4. adenosine triphosphate
5. 14mm

RESULTS:
(+) Streptococcus pneumoniae
(-) Viridans

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6
Q

BILE SOLUBILITY TEST
______ vs _____

  1. ____ lyses pneumococcal colonies
    - example of bile
    - ____ on well isolated colony
    - ____ on tube test
  2. intracellular autolytic enzyme
  3. ____ surface tension = _____ lysis of cell
  4. positive results
  5. negative results

RESULTS:
(+)
(-)

A

s.pneumoniae vs other alpha (viridans)

  1. bile
    - sodium deoxycholate
    - 10% of sodium deoxycholate
    - 2% of sodium deoxycholate
  2. amidase
  3. decreased surface tension = increased lysis of cell
  4. colony disintegrates
  5. intact colonies

RESULTS:
(+) Streptococcus pneumoniae
(-) Viridans

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7
Q

BILE ESCULIN TEST
______ vs _____

  1. capable of growth in the presence of ___%
  2. able to hydrolyze _____ to ____
  3. the product reacts w/ _____
  4. positive results
  5. negative results

RESULTS:
(+)
(-)

A

enterococcus vs viridans
1. 4% bile
2. esculin to esculetin
3. ferric ion
4. growth & blackening of agar slant
5. no blackening

RESULTS:
(+) Enterococcus
(-) Viridans

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8
Q

SALT TOLERANCE TEST
______ vs _____

  1. uses _____ as a medium
  2. ## contains:
    • indicator for acid)
  3. positive results
  4. negative results

RESULTS:
(+)
(-)

A

enterococci vs non enterococci

  1. 6.5% sodium chloride
    2.
    - glucose
    - bromocresol purple
  2. visible turbidity in broth, w/ or w/o color change from purple to yellow
  3. no turbidity and no color change

RESULTS:
(+) Enterococcus
(-) group D streptococcus (Streptococcus bovis)

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9
Q

PYR TEST
______ vs _____

  1. positive results
  2. negative results

RESULTS:
(+)
(-)

A

enterococci vs non enterococci (group D streptococci)

  1. bright red color within 5mins
  2. no color change or an orange color

RESULTS:
(+) Enterococcus
(-) group D streptococcus

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10
Q

_____: presumptive identification of catalase-negative
- aka

  1. ______ serves as substrate
  2. detection of _____
  3. results to _____ produces red
  4. reagent
  5. positive result
  6. negative result

RESULTS:
(+)
(-)

A

LAP TEST
- LEUCINE AMINOPEPTIDASE

  1. leucine-beta-naphthylamide–impregnated disks
  2. leucine-beta-naphthylamide–impregnated disks
  3. beta-naphthylamine
  4. cinnamaldehyde reagent
  5. development of red color within 1min after adding cinnamaldehyde reagent
  6. no color change or development of slight yellow color

RESULTS:
(+) all Streptococcus
(-)

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11
Q

_____: rapid method to differentiate staph vs micrococci
- aka

  1. detection of the enzyme ______
  2. oxidase enzyme reacts w/ ____ & ____
  3. results to _____ producing colored
  4. positive result
  5. negative result

RESULTS:
(+)
(-)

A

MICRODASE
- MODIFIED OXIDASE

  1. oxidase
  2. oxidase reagent & cytochrome C
  3. indophenol
  4. development of blue to purple-blue color
  5. no color change or development of slight yellow color

RESULTS:
(+) Micrococci
(-) all Staphylococcus except S. sciuri, S. lentus, and S. vitulus

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12
Q

_____: detection of s.pneumoniae & serotyping of isolates
- aka

  1. : capsule will swell in presence of ______
  2. specimen
  3. isolated colony will be mixed w/ ___ & ___ (______)
  4. stain w/ ______
  5. positive result
  6. negative result

RESULTS:
(+)
(-)

A

NEUFELD-QUELLUNG REACTION (CAPSULAR SWELLING TEST)

  1. specific anti capsular serum
  2. sputum
  3. saline & reagent (anticapsular serum)
  4. methylene blue
  5. outlines or halo around bacteria
  6. no capsular swelling/ no clumping

RESULTS:
(+) pneumococci
(-) N/A

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