GRAM POSITIVE COCCI.2 Flashcards
BACITRACIN
1. _____ vs ______
2. ZOI mm
RESULTS:
(+)
(-)
- group A vs group B
- 10mm
RESULTS:
(+) Streptococcus pyogenes (Group A)
(-) Streptococcus agalactiae (Group B)
PYR
- aka
- ____ vs _____
- enzyme:
- enzyme hydrolyzes _______
- to produce _______
- reagent
- positive results
RESULTS:
(+)
(-)
PYR
- I-PYRROLIDONYL ARYLAMIDASE TEST
- group A & entero vs group B
- l-pyrrolidonyl arylamidase
- l-pyrrolidonyl-beta-naphthylamide
- beta-naphthylamine
- N,N-methylamino-cinnamaldehyde reagent
- bright red color within 5mins
- no color change or an orange color
RESULTS:
(+) Streptococcus pyogenes (Group A) & Enterococcus
(-) Streptococcus agalactiae (Group B)
CAMP
1. aka
2. ___ vs _____
3. produces _______
4. the product acts synergistically w/ ______
- to cause enhanced lysis of ___
5. positive results
6. negative results
RESULTS:
(+)
(-)
CAMP
1. CHRISTIE, ATKINS, MUNCH-PETERSON (CAMP) TEST
2. group A vs group B
3. diffusible extracellular hemolytic protein (CAMP,factor)
4. beta-lysin of S. aureus
- RBCs
5. enhanced hemolysis is indicated by arrowhead-shaped zone
6. no enhancement of hemolysis
RESULTS:
(+) Streptococcus agalactiae (Group B)
(-) Streptococcus pyogenes (Group A)
HIPPURATE HYDROLYSIS
1. ____ vs _____
2. end products of hydrolysis of hippuric acid by ____
- includes (2)
3. ____ is deaminated by _____ (oxidizing agent)
4. medium must contain only ______
- as ninhydrin might react w/ any _____ present
5. positive result
6. negative result
RESULTS:
(+)
(-)
- group A vs group B
- hippuricase
- glycine & benzoic acid - glycine; ninhydrin
- hippurate
- free amino acids - deep purple color
- colorless or slightly yellow pink color
RESULTS:
(+) Streptococcus agalactiae (Group B)
(-) Streptococcus pyogenes (Group A)
OPTOCHIN
______ vs _____
1. aka
2. optochin (______)
3. interferes with ____
4. production of
5. ZOI mm
RESULTS:
(+)
(-)
s.pneumoniae (presumptive identification) vs other alpha (viridans)
1. TAXO P
2. ethylhydrocupreine hydrochloride
3. ATPase
4. adenosine triphosphate
5. 14mm
RESULTS:
(+) Streptococcus pneumoniae
(-) Viridans
BILE SOLUBILITY TEST
______ vs _____
- ____ lyses pneumococcal colonies
- example of bile
- ____ on well isolated colony
- ____ on tube test - intracellular autolytic enzyme
- ____ surface tension = _____ lysis of cell
- positive results
- negative results
RESULTS:
(+)
(-)
s.pneumoniae vs other alpha (viridans)
- bile
- sodium deoxycholate
- 10% of sodium deoxycholate
- 2% of sodium deoxycholate - amidase
- decreased surface tension = increased lysis of cell
- colony disintegrates
- intact colonies
RESULTS:
(+) Streptococcus pneumoniae
(-) Viridans
BILE ESCULIN TEST
______ vs _____
- capable of growth in the presence of ___%
- able to hydrolyze _____ to ____
- the product reacts w/ _____
- positive results
- negative results
RESULTS:
(+)
(-)
enterococcus vs viridans
1. 4% bile
2. esculin to esculetin
3. ferric ion
4. growth & blackening of agar slant
5. no blackening
RESULTS:
(+) Enterococcus
(-) Viridans
SALT TOLERANCE TEST
______ vs _____
- uses _____ as a medium
- ## contains:
- indicator for acid)
- positive results
- negative results
RESULTS:
(+)
(-)
enterococci vs non enterococci
- 6.5% sodium chloride
2.
- glucose
- bromocresol purple - visible turbidity in broth, w/ or w/o color change from purple to yellow
- no turbidity and no color change
RESULTS:
(+) Enterococcus
(-) group D streptococcus (Streptococcus bovis)
PYR TEST
______ vs _____
- positive results
- negative results
RESULTS:
(+)
(-)
enterococci vs non enterococci (group D streptococci)
- bright red color within 5mins
- no color change or an orange color
RESULTS:
(+) Enterococcus
(-) group D streptococcus
_____: presumptive identification of catalase-negative
- aka
- ______ serves as substrate
- detection of _____
- results to _____ produces red
- reagent
- positive result
- negative result
RESULTS:
(+)
(-)
LAP TEST
- LEUCINE AMINOPEPTIDASE
- leucine-beta-naphthylamide–impregnated disks
- leucine
aminopeptidase - beta-naphthylamine
- cinnamaldehyde reagent
- development of red color within 1min after adding cinnamaldehyde reagent
- no color change or development of slight yellow color
RESULTS:
(+) all Streptococcus
(-)
_____: rapid method to differentiate staph vs micrococci
- aka
- detection of the enzyme ______
- oxidase enzyme reacts w/ ____ & ____
- results to _____ producing colored
- positive result
- negative result
RESULTS:
(+)
(-)
MICRODASE
- MODIFIED OXIDASE
- oxidase
- oxidase reagent & cytochrome C
- indophenol
- development of blue to purple-blue color
- no color change or development of slight yellow color
RESULTS:
(+) Micrococci
(-) all Staphylococcus except S. sciuri, S. lentus, and S. vitulus
_____: detection of s.pneumoniae & serotyping of isolates
- aka
- : capsule will swell in presence of ______
- specimen
- isolated colony will be mixed w/ ___ & ___ (______)
- stain w/ ______
- positive result
- negative result
RESULTS:
(+)
(-)
NEUFELD-QUELLUNG REACTION (CAPSULAR SWELLING TEST)
- specific anti capsular serum
- sputum
- saline & reagent (anticapsular serum)
- methylene blue
- outlines or halo around bacteria
- no capsular swelling/ no clumping
RESULTS:
(+) pneumococci
(-) N/A
