Biosafety

Question 1

Classification of Infective Microorganisms by Risk Group

Risk Group 1
Risk Group 2
Risk Group 3
Risk Group 4

Answer 1

now/low ndiv & community risk
moderate indiv risk & low community risk
high Indiv risk, low community risk
high indiv risk, high community risk

Question 2

BSL:

1. no known pathogenic potential for immunocompetent individuals
   - inc.
2. most common microorganisms associated w/ lab acquired infections
   - inc. (4)
3. air movement must be carefully controlled
   - inc (4)
4. research facilities
   - inc (2)

Answer 2

1. BSL 1
   - Bacillus subtilis
2. BSL 2
   - HBV, HIV, Staph, enteric (Salmonella & Shigella)
3. BSL 3
   - M.tuberculosis, Brucella, Coccidioides, Rickettsiae, specific virus (arbovirus)
4. BSL 4
   - exotic virus (filovirus,arenavirus)

Question 3

Potential Infectious Agents of Bioterrorism
1. poses the greatest public health threat; easily transmitted & highly
infectious
- (4)
2. tendency to cause a disease but low mortality rate; not easily
transmitted
- (3)
3. emerging pathogenic agents
- (3)

Answer 3

1. CATEGORY A
   - Smallpox, anthrax, bacillus, F. tularensis
2. CATEGORY B
   - Coxiella burnetti, Burkholderia pseudomallie, Rickettsia
3. CATEGORY C
   -Yellow fever virus, dengue virus,
   hemorrhagic fever virus

Question 4

______: the air that is exhausted is the only thing that is sterilized
1. protects the ______ & _____ but not the ______

_____: will sterilize the air that flows in the infectious material and even the air to be exhausted
1. aka
2. utilized in _____
3. types:
- fixed opening
- variable opening

____: provides the most protection
1. utilized during ____ agents

Answer 4

BSC 1
1. protects the worker and environment but not the organism

BSC 2
1. vertical laminar
2. microbiology
3. types:
- Class IIA
- Class IIB

BSC 3
1. BSL4 agents

Question 5

Thermometer
1. checked ____ against reference thermometer
2. presence of gas bubbles so check ____
3. tolerance limits
- Refrigerators
- Freezers
- Incubators
- Waterbath
4. temp for calibration (4)

Answer 5

1. periodically
2. Daily
3. tolerance limits
   - 2-8C
   - -8 to -20C or -60 to -75C
   - 35.5C +/-1C
   - 36-38C or 55-57C
4. -20, 2-8, 37, 56C

Question 6

Waterbath
1. check
2. clean

CO2 incubator & regular
1. ____ monitoring of temp
2. percentage of co2 checked ___5

Centri
1. RPM must be checked ____

Autoclaves
1. test w/______
2. checked ______

Reagents
1. tested ______ w/ pos & neg controls

Answer 6

Waterbath
1. DAILY
2. MONTHLY

CO2 incubator & regular
1. DAILY
2. DAILY

Centri
1. 2x a YEAR

Autoclaves
1. SPORE STRIPS
2. WEEKLY

Reagents
1. DAILY

Question 7

Culture media
1. Keep for at least ____
2. Compare w/

Antimicrobial susceptibility drugs
1. daily for _____ days
2. control organism from ATCC ____

Stock culture
1. _____ viability
- should be subcultured ____ after thawing
2. stored at

Answer 7

Culture media
1. 2 YEARS
2. FASTIDIOUS

Antimicrobial susceptibility drugs
1. 20-30 DAYS
2. AMERICAN TYPE CULTURE COLLECTION

Stock culture
1. YEAR
- TWICE
2. -70C