GRAM NEGATIVE COCCI:

Question 1

Intro:
1. oxygen require,end
2. catalase _____
- required hydrogen peroxide
- except ____
3. oxidase ___

Answer 1

1. strictly aerobic
2. positive
   - 30% hydrogen peroxide
   - N.elongata
3. positive

Question 2

Specimen Considerations:
1. from _____
2. swab _____
3. transport system: JEMBEC
- JEMBEC stands for
- other 2
- JEMBEC components: ______ + _______ (_____ + _____)
- incubator: _____ or _____

Answer 2

1. genital sources
2. dacron/ rayon swab
   3.
   - James E. Martin Biological Environmental Chamber
   - Gono-Pak, Transgrow
   - Thayer-Martin + CO2-generating tablet (sodium bicarbonate + citric acid)
   - CO2 incubator or Glass jar

Question 3

N.gonorrhoeae

1. gram negative _________
2. from ______
3. > 5PMN filed with no bacteria = _________
4. utilizes ________
   - morphology

N.menigitidis
1. gram negative __________
2. from:
- sterile
- nonsterile
4. utilizes ______ & _____
- morphology

Answer 3

N.gonorrhea
1. INTRACELLULAR diplococci
2. urogenital specimen
3. nongonococcal urethritis
4. Chocolate Agar Plate
- small, gray to tan, translucent, raised

N.menigitidis
1. INTRACELLULAR & EXTRACELLULAR diplococci
2.
- CSF (by BBB); blood
- nasopharyngeal, aspirates, sputum, urogenital sites
4. Chocolate Agar Plate & Sheep Blood Agar
- medium sized, gray, convex, mucoid

Question 4

SELECTIVE MEDIA

1. gram positive inhibitor
2. gram negative inhibitor
3. swarming inhibitor (proteus)
4. fungi inhibitor

Answer 4

1. vancomycin
2. colistin w/ lincomycin
3. trimethoprim
4. amphotericin B w/ nystatin

Question 5

Other Selective Media
1. Thayer-Martin
2. Modified Thayer-Martin
3. Martin-Lewis
4. New York City
5. GC-LECT

Answer 5

1. VANCOMYCIN, COLISTIN, NYSTATIN
2. VANCOMYCIN, COLISTIN, NYSTATIN, TRIMETHOPRIM
3. VANCOMYCIN, COLISTIN, TRIMETHOPRIM, ANISOMYCIN
4. VANCOMYCIN, COLISTIN, TRIMETHOPRIM, AMPHOTERICIN B
5. VANCOMYCIN, COLISTIN, TRIMETHOPRIM, AMPHOTERICIN B, LINCOMYCIN

Question 6

OXIDASE TEST:
1. determine the presence of _________
- using ______ rxn
2. substrate _______
- or ______
3. reagent
- color end
4. positive
5. negative

Answer 6

1. bacterial cytochrome oxidase
   - oxidation (redox rxn)
2. dimethyl-p-phenylenediamine-dihydrochloride
   - tetramethyl-p-phenylenediamine dihydrochloride
3. indophenol
   - dark purple
4. development of deep purple to black
5. no color change

Question 7

Definitive tests
1. uses 1% conc of each sugar
- medium
- indicator
- incubator
- positive
2. can identify different strains of Neisseria
3. can combine enzyme and biochemical tests to identify Neisseria

Answer 7

1. Carbohydrate Method
   - CYSTINE TRYPTICASE AGAR
   - phenol red
   - regular incubator
   - yellow color
2. Chromogenic Enzyme Methods
3. Multitest Assay

Question 8

CARBOHYDRATE METHOD results
1. Gonorrhea
2. Meningitidis
3. Lactamica
4. Cinerea
5. Sicca
6. Flavescens

Answer 8

1. G
2. G,M
3. G,M,L
4. G
5. G.M.S.F
6. none

Question 9

ENZYME METHOD: BGH

1. Gonorrhea
2. Meningitidis
3. Lactamica
4. Cinerea
5. Sicca
6. Flavescens

Answer 9

1. -,-,+
2. +,+,-
3. +,-,-
4. -,-,+
5. -,-,NT
6. NT,NT,+

Question 10

Moraxella catarrhalis

1. utilizes ______
   - morphology
2. when colony is moved with loop, it also moves
3. observed in older colonies

Identification results:
1. oxidase ___
2. catalase ____
3. butyrate esterase ______
4. incapable of breaking down carbohydrates
5. this differentiates them from Neisseria

Answer 10

1. Chocolate Agar Plate & Sheep Blood Agar
   - smooth, opaque, gray to white colonies
2. hockey puck appearance
3. wagon wheel appearance

Identification results:
1. OXIDASE POSITIVE
2. CATALASE POSITIVE
3. BUTYRATE ESTERASE POSITIVE
4. ASACCHAROLYTIC
5. DNASE POSITIVE