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Microbiology > Gram Negative Cocci > Flashcards

Gram Negative Cocci Flashcards

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Biology 101 flashcards
1
Q

Anaerobic Gram Negative Cocci

A

Veionella

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2
Q

Old name of Neisseria

A

Streptococcus

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3
Q

Presumptive Test for Neisseria

A

Oxidase Test

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4
Q

(+) CAP

(-) BAP

A

N. gonorrheae

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5
Q

(+) CAP

(+) BAP

A

N. meningitides

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6
Q

Yellow-Pigmented Neisseria

A

N. subflava/N. flavescens

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7
Q

Obligate aerobe, capnophilic, non-motile, no -hemolytic and are carbohydrate fermenters

A

Neisseria

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8
Q

Neisseria gonorrheae requires ____ for growth.

A

Iron

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9
Q

Principal virulence factor of Neisseria

A

Common pili

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10
Q

Important in Neisseria’s pathogenesis in mucous membrane secretions

A

IgA Protease

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11
Q

Virulent colonial types of Neisseria

A

T1 and T2

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12
Q

Avirulent colonial types of Neisseria

A

T3 and T5

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13
Q

Means a “flow of seed” and “brothel”

Acute pyogenic infection of nonciliated columnar and transitional epithelium

A

Gonorrhea

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14
Q

3 causative agents of PID

A
  1. C. trachomatis
  2. N. gonorrheae
  3. M. hominis
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15
Q

CA of Fitz-Hugh-Curtis syndrome

A

Neisseria gonorrheae

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16
Q

CA of opthalmia neonatorum

A

N. gonorrheae

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17
Q

Transport medium for Neisseria

A

Amies with charcoal
Transgrow
Cary Blair
JEMBEC

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18
Q

Swabs for Neisseria should be plated within ____ hours

A

6 hours

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19
Q

True or false

Neisseria will not be recovered from routine blood cultures because it is inhibited by SPS

A

True

Inhibited by SPS: (Sm PaNGV)

S. monoliformis
P. anaerobius
Neisseria
G. vaginalis

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20
Q

Neisseria is a cold-sensitive organism. Thus, specimens should be kept at RT or placed ar 37 C before plating.

True or False

A

True

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21
Q

The appearance of intracellular Gram-negative diplococci, kidney or coffee-bean shaped is DIAGNOSTIC.

True or False

A

True

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22
Q

> 5 PMNs/field but no bacteria is indicative of

A

Non gonococcal urethritis (C. trachomatis)

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23
Q

JEMBEC stands for

A

John E. Martin Biological Environment Chamber

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24
Q

Antimicrobial agents in TMA

A

Vancomycin
Colistin
Nystatin

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25
Q

Antimicrobial agents in MTM

A

All TMA components + Trimethoprim Lactate

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26
Q

Trimethoprim Lactate inhibits

A

Swarming of Proteus

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27
Q

Antimicrobial agents in Martin Lewis Medium (ML)

A

Vancomycin
Colistin
Anisomycin

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28
Q

Anisomycin in ML inhibits the growth of

A

Yeast

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29
Q

A transparent Gonoccocal medium

A

New York City Medium

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30
Q

Antimicrobial agents in NYC medium

A

Vancomycin
Colistin
Amphotericin B
Trimethoprim

(VCAT)

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31
Q

Antimicrobial agents in GC-LECT medium

A

NYC antibiotics + Lincomycin

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32
Q

Other bacteria that can grow in gonococcal media (3)

A

Si King nakaCAP sa Cine

Kingella denitrificans
Capnocytophaga
Acinetobacter

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33
Q

Standard method of identifying N. gonorrhea

A

CHO utilization test

34
Q

Medium used in CHO utilization test

A

Cystine Trypticase Agar (CTA)

35
Q

CTA consists of

A

1% CHO + Phenol Red

36
Q

+ result in CHO utilization test

A

Yellow color

37
Q

Reagent in Oxidase Test

A

1% tetramethyl-p-phenylenediamine dihydrochloride

38
Q

Positive result in oxidase test

A

Purple color within 10 seconds

39
Q

Reagent in superoxol test/modified catalase test

A

20-30% H202

40
Q

Positive result in superoxol test

A

Vigorous Bubbling

41
Q

Causative agent of Clap

A

Clap = Gonorrhea

N. gonorrheae

42
Q

Taxo N

A

Cytochrome Oxidase

Indophenol Blue

43
Q

Oxidase Test can be done in (3)

A
  1. colony
  2. filter paper
  3. Disk
44
Q

True or False

CTA in CHO utilization/fermentation test should be placed in a Non-CO2 incubator

A

True

45
Q

Choromogenic cephalosporinase test (+) result

A

Pink/Red

46
Q

Iodometric test + result

A

Colorless

47
Q

Acidimetric + result

A

Yellow

48
Q

Most sensitive beta lactamase test

A

Nitrocetin Test

49
Q

Positive result in beta lactamase test

A

Color change

50
Q

Vancomycin inhibits

A

Gram +

51
Q

Colistin inhibits

A

Gram -

52
Q

Nystatin inhibits

A

Yeast

53
Q

Amphotericin B inhibits

A

Yeast

54
Q

CA of Meningococcemia

A

N. meningitidis

55
Q

Serotypes of N. meningitidis

A

A, B, C, Y, and W-135

56
Q

CA of DIC

A

N. meningitidis

57
Q

CA of Waterhouse-Friderichsen Syndrome (hemorrhage in the adrenals)

A

N. meningitids

58
Q

Gamma-glutamyl aminopeptidase test (+)

A

N. meningitidis

59
Q

Rod-shaped gram negative cocci that is weakly + or - in catalase test

A

N. elongata

60
Q

Rod-shaped gram negative cocci that is weakly PAD +

A

N. weaveri

61
Q

Normal flora of the URT

A

M. catarrhalis

62
Q

3rd cause of otitis media

A

Moraxella catarrhalis

63
Q

“honey puck apperance” colonies

A

M. catarrhalis

64
Q

Wagon wheel appearance colonies

A

Old colonies of M. catarrhalis

65
Q

M. catarrhalis grows on BAP at ___ C and on nutrient agar at ____ C

A

22

35

66
Q

Assacharolytic in CHO degradation test

A

M. catarrhalis

67
Q

DNAse (+)

A

M. catarrhalis

68
Q

Glucose fermenter

A

N. gonorrheae

69
Q

Glucose and Maltose fermenter

A

N. meningitidis

70
Q

ONPG (+)

Glucose, Maltose, and Lactose fermenter

A

N. lactamica

71
Q

Wrinkled colony

Glucose, Maltose and Sucrose fermenter

A

N. secca

72
Q

DNT (+)

DNAse
Nitrate Reduction Test
Tributyrin Hydrolysis Test

A

M. catarrhalis

73
Q

Old name of Glucose

A

Dextrose

74
Q

Old name of Sucrose

A

Saccharose

75
Q

Butyrate esterase test/Butyrate Disk/Tributyrin HOH test (+) result

A

Blue color

76
Q

Positive in Butyrate esterase test/Butyrate Disk/Tributyrin HOH test

A

M. catarrhalis

77
Q

Specimen used in Butyrate esterase test/Butyrate Disk/Tributyrin HOH test

A

Eye or eye culture

78
Q

3 organisms causing Otitis Media

A
  1. S. pneumoniae
  2. H. influenzae
  3. M. catarrhalis
79
Q

B-galactosidase (+)

A

N. lactamica

80
Q

Superoxol (+)

A

N. gonorrheae

Microbiology (9 decks)

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