Gram Negative Cocci Flashcards
Anaerobic Gram Negative Cocci
Veionella
Old name of Neisseria
Streptococcus
Presumptive Test for Neisseria
Oxidase Test
(+) CAP
(-) BAP
N. gonorrheae
(+) CAP
(+) BAP
N. meningitides
Yellow-Pigmented Neisseria
N. subflava/N. flavescens
Obligate aerobe, capnophilic, non-motile, no -hemolytic and are carbohydrate fermenters
Neisseria
Neisseria gonorrheae requires ____ for growth.
Iron
Principal virulence factor of Neisseria
Common pili
Important in Neisseria’s pathogenesis in mucous membrane secretions
IgA Protease
Virulent colonial types of Neisseria
T1 and T2
Avirulent colonial types of Neisseria
T3 and T5
Means a “flow of seed” and “brothel”
Acute pyogenic infection of nonciliated columnar and transitional epithelium
Gonorrhea
3 causative agents of PID
- C. trachomatis
- N. gonorrheae
- M. hominis
CA of Fitz-Hugh-Curtis syndrome
Neisseria gonorrheae
CA of opthalmia neonatorum
N. gonorrheae
Transport medium for Neisseria
Amies with charcoal
Transgrow
Cary Blair
JEMBEC
Swabs for Neisseria should be plated within ____ hours
6 hours
True or false
Neisseria will not be recovered from routine blood cultures because it is inhibited by SPS
True
Inhibited by SPS: (Sm PaNGV)
S. monoliformis
P. anaerobius
Neisseria
G. vaginalis
Neisseria is a cold-sensitive organism. Thus, specimens should be kept at RT or placed ar 37 C before plating.
True or False
True
The appearance of intracellular Gram-negative diplococci, kidney or coffee-bean shaped is DIAGNOSTIC.
True or False
True
> 5 PMNs/field but no bacteria is indicative of
Non gonococcal urethritis (C. trachomatis)
JEMBEC stands for
John E. Martin Biological Environment Chamber
Antimicrobial agents in TMA
Vancomycin
Colistin
Nystatin
Antimicrobial agents in MTM
All TMA components + Trimethoprim Lactate
Trimethoprim Lactate inhibits
Swarming of Proteus
Antimicrobial agents in Martin Lewis Medium (ML)
Vancomycin
Colistin
Anisomycin
Anisomycin in ML inhibits the growth of
Yeast
A transparent Gonoccocal medium
New York City Medium
Antimicrobial agents in NYC medium
Vancomycin
Colistin
Amphotericin B
Trimethoprim
(VCAT)
Antimicrobial agents in GC-LECT medium
NYC antibiotics + Lincomycin
Other bacteria that can grow in gonococcal media (3)
Si King nakaCAP sa Cine
Kingella denitrificans
Capnocytophaga
Acinetobacter
Standard method of identifying N. gonorrhea
CHO utilization test
Medium used in CHO utilization test
Cystine Trypticase Agar (CTA)
CTA consists of
1% CHO + Phenol Red
+ result in CHO utilization test
Yellow color
Reagent in Oxidase Test
1% tetramethyl-p-phenylenediamine dihydrochloride
Positive result in oxidase test
Purple color within 10 seconds
Reagent in superoxol test/modified catalase test
20-30% H202
Positive result in superoxol test
Vigorous Bubbling
Causative agent of Clap
Clap = Gonorrhea
N. gonorrheae
Taxo N
Cytochrome Oxidase
Indophenol Blue
Oxidase Test can be done in (3)
- colony
- filter paper
- Disk
True or False
CTA in CHO utilization/fermentation test should be placed in a Non-CO2 incubator
True
Choromogenic cephalosporinase test (+) result
Pink/Red
Iodometric test + result
Colorless
Acidimetric + result
Yellow
Most sensitive beta lactamase test
Nitrocetin Test
Positive result in beta lactamase test
Color change
Vancomycin inhibits
Gram +
Colistin inhibits
Gram -
Nystatin inhibits
Yeast
Amphotericin B inhibits
Yeast
CA of Meningococcemia
N. meningitidis
Serotypes of N. meningitidis
A, B, C, Y, and W-135
CA of DIC
N. meningitidis
CA of Waterhouse-Friderichsen Syndrome (hemorrhage in the adrenals)
N. meningitids
Gamma-glutamyl aminopeptidase test (+)
N. meningitidis
Rod-shaped gram negative cocci that is weakly + or - in catalase test
N. elongata
Rod-shaped gram negative cocci that is weakly PAD +
N. weaveri
Normal flora of the URT
M. catarrhalis
3rd cause of otitis media
Moraxella catarrhalis
“honey puck apperance” colonies
M. catarrhalis
Wagon wheel appearance colonies
Old colonies of M. catarrhalis
M. catarrhalis grows on BAP at ___ C and on nutrient agar at ____ C
22
35
Assacharolytic in CHO degradation test
M. catarrhalis
DNAse (+)
M. catarrhalis
Glucose fermenter
N. gonorrheae
Glucose and Maltose fermenter
N. meningitidis
ONPG (+)
Glucose, Maltose, and Lactose fermenter
N. lactamica
Wrinkled colony
Glucose, Maltose and Sucrose fermenter
N. secca
DNT (+)
DNAse
Nitrate Reduction Test
Tributyrin Hydrolysis Test
M. catarrhalis
Old name of Glucose
Dextrose
Old name of Sucrose
Saccharose
Butyrate esterase test/Butyrate Disk/Tributyrin HOH test (+) result
Blue color
Positive in Butyrate esterase test/Butyrate Disk/Tributyrin HOH test
M. catarrhalis
Specimen used in Butyrate esterase test/Butyrate Disk/Tributyrin HOH test
Eye or eye culture
3 organisms causing Otitis Media
- S. pneumoniae
- H. influenzae
- M. catarrhalis
B-galactosidase (+)
N. lactamica
Superoxol (+)
N. gonorrheae