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Microbiology > Streptococci > Flashcards

Streptococci Flashcards

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1
Q

Classification of streptocci based on temperature requirement

A

Academic/Bergey’s classification

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2
Q

Pyogenic bacteria temperature requirement

A

37 C

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3
Q

Viridans temperature requirement

A

37 C or 45 C

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4
Q

Enterococcus temperature requirement

A

10 C, 37 C, 45 C

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5
Q

Lactic group temperature requirement

A

10 C, 37 C

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6
Q

Classification of bacteria based on hemolytic patterns

A

Smith and Brown Classification

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7
Q

Green hemolysis

A

Alpha hemolysis

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8
Q

Clear zone of hemolysis

A

Beta hemolysis

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9
Q

No hemolysis

A

Gamma hemolysis

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10
Q

Based on the extraction of C Carbohydrate from the streptococcal cell wall

A

Lancefield Classification (Rebecca Lancefield)

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11
Q

Beta hemolytic, Group A

A

S. pyogenes

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12
Q

Beta hemolytic, Group B

A

S. agalactiae

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13
Q

Beta hemolytic, Group C

A

S. dysagalactiae subsp. equisimilus, S. equi

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14
Q

Alpha / Gamma hemolytic, Group D

A

S. bovis

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15
Q

Alpha/Beta/Gamma hemolytic Group D

A

Enterococci

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16
Q

Alpha hemolytic, No Lancefield Classification

A

S. pneumoniae

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17
Q

Fever producing bacteria

A

S. pyogenes

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18
Q

Flesh-eating bacteria

A

S. pyogenes

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19
Q

Principal virulence factor of Group A strep

A

M Protein

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20
Q

Oxygen labile hemolysin of Group A

A

Streptolysin O

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21
Q

Oxygen stable toxin of Group A

A

Streptolysin S

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22
Q

Hemolysin responsible for subsurface hemolysis on BAP

A

Streptolysin O

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23
Q

Hemolysin responsible for the surface hemolysis on BAP

A

Streptolysin S

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24
Q

Formerly known as erythrogenic toxins

A

Pyrogenic Toxins

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25
Q

Cysteine protease which mediates rash in scarlet fever

A

Exotoxin B

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26
Q

Specimen for group A strep

A

Throat Swab

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27
Q

Causative agent of Strep Throat or Pharyngitis

A

Group A strep

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28
Q

Strawberry-colored tongue

A

Scarlet Fever

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29
Q

Susceptibility Test for Scarlet Fever

A

Dick’s Test

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30
Q

+ result in Dick’s Test

A

Erythema or redness of the test site

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31
Q

Diagnostic Test for Scarlet Fever

A

Schultz Charlton (Anti-erythrogenic Toxin)

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32
Q

Positive result in Schultz-Charlton Test

A

Blanching Phenomenon (Rash Fade)

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33
Q

Causative agent of Necrotizing Fasciitis/Galloping Gangrene

A

S. pyogenes

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34
Q

Also known as Bright’s disease

A

Acute Glomerulonephritis

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35
Q

Post-streptococcal sequelae (Group A)

A

AGN

Rheumatic Fever

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36
Q

4 confirmatory tests for Strep

A
  1. lancefield test
  2. Fluorescent Ab Test
  3. PHADEBACT
  4. Neufeld Quellung Test (S. pneumoniae)
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37
Q

Normal flora of the female genital tract and lower GIT

A

Group B strep

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38
Q

Causative agent of Neonatal Meningitis

A

Group B strep (S. agalactiae)

39
Q

Virulence factor of Group B strep

A

Sialic Acid

40
Q

Positive result in CAMP test

A

Arrow head B hemolysis or bowtie appearance

41
Q

Reagents for Hippurate Hydrolysis Test

A

Sodium hippurate and Ninhydrin

42
Q

Positive result in Hippurate Hydrolysis Test

A

Purple Color

43
Q

Negative control in CAMP test

A

Enterococci

44
Q

Reagent in CAMP test

A

B-lysin-producimg strain of S. aureus or B-lysin disk

45
Q

Most common cause of SBE

A

Viridans strep

46
Q

Most commonly isolated viridans strep

A

S. mutans

47
Q

a.k.a wide zone hemolysis

Colonies are surrounded by a very small zone of no hemolysis and then a wider zone of B-hemolysis

A

Alpha-prime hemolysis

48
Q

Most common isolate of enterococci

A

E. faecalis

49
Q

Reagent in Bile Esculin test

A

Esculin + 1-4% bile salt

50
Q

+ result in bile esculin medium

A

Black color complex in the agar

51
Q

Positive result in 6.5% NaCl test

A

Turbidity

52
Q

E. faecalis growth factor requirement

A

Cyanocobalamin (B12)

53
Q

Carrot broth is a selective broth for

A

S. agalactiae

54
Q

Has a characteristic “sweet odor of honeysuckle or butterscotch” when grown in high concentration or pure culture

A

S. anginosus group

55
Q

Catalase test result when colonies are taken from BAP

A

False +

56
Q

Positive result in LAP test

A

Red color

57
Q

Reagent in VP Test

A

5% alpha naphthol

40% KOH

58
Q

Diplococcus streptococci

A

S. pneumoniae

59
Q

Causative agent of Lobar pneumonia

A

S. pneumoniae

60
Q

Most common cause of Adult Meningitis

A

S. pneuomoniae

61
Q

Virulence factor of S. pneumoniae

A

Capsular polysaccharide

62
Q

Appearance of S. pneumoniae microscopically

A

Lancet-shaped

63
Q

“Coin with a raised rim”

Dimple-shaped/donut-shaped colonies

A

Old colonies of S. pneumoniae

64
Q

“Dome-shaped” colonies

A

Young colonies of S. pneumoniae

65
Q

Streptococcus causing Otitis Media

A

S. pneumoniae

66
Q

Presumptive test for S. pneumoniae

A

Taxo P/Optochin susceptibility test

67
Q

Reagent for Taxo P

A

Ethylhydroxycuprein hydrochloride

68
Q

Positive result in Taxo P

A

> or = 14mm

69
Q

Confirmatory test for S. pneumoniae which detects the activity of amidase

A

Bile solubility

70
Q

Reagent in Bile Solubility Test

A

Sodium desoxycholate (bile salt)

71
Q

+ result in bile solubility test

A

No turbidity

72
Q

Formerly known as nutritionally variant streptococci (NVS)

A

Abiotrophia

Granulicatella

73
Q

Bacitracin (S)
PYR (+)
SXT (R)

A

S. pyogenes

74
Q

CAMP (+)
Hippurate HOH (+)
SXT (R)

A

Group B strep

75
Q

SXT (S)

A

Group C, F, and G

Rodriguez book: Also S. pneumoniae and Viridans

76
Q 
Gamma hemolytic
Bile esculin(+)
A

Group D strep

77
Q

Alpha hemolytic
Optochin (S)
Bile solubility (+)

A

S. pneumoniae

78
Q

Alpha hemolytic
Optochin (R)
Bile solubility (-)

A

S. viridans

79
Q

Growth i. 6.5% NaCl (+)

A

Enterococci

80
Q

PYR (+)

A

Group A strep and Group D enterococci

81
Q

Bile solubility reagent on BAP and positive result

A 
10% sodium desoxycholate
Lyzed colony (+)
82
Q

Bile solubility reagent in tube and positive result

A

2% sodium desoxycholate

Clear (+)

83
Q

Test to differentiate S. viridans from Pediococcus/Leuconostoc

A

Vancomycin Resistance Test

S. viridans (S)

84
Q

MRS Broth test (+) result

A

Gas formation
(+) Leuconostoc spp
(-) Pediococcus

85
Q

Thrombolytic drug for AMI from strep

A

Streptokinase

86
Q

1 cause of Impetigo

A

S. pyogenes

87
Q

Causative agent of Puerperal Sepsis and Childbed Fever

A

S. pyogenes

88
Q

Microscope used in Todd Hewitt Broth

A

Fluorescence microscope

89
Q

Nosocomial pathogen

A

Enterococcus

90
Q

CA of Colon Cancer

A

S. bovis

91
Q

1 normal flora of the mouth/oral cavity

A

Viridans streptococci

92
Q

CA of SBE due to dental procedure

A

S. sanguis

93
Q

Test to differentiate Enterococcus (+) from Pediococcus (-)

A

PYR Test

94
Q 
Requires Vit. B6 (Pyridoxine)
Staph streak (+)
A

NVS
Nutritionally Variant Strep
(Abiotrophia and Granulicatella)

Microbiology (9 decks)

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