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Microbiology > Basic Micro > Flashcards

Basic Micro Flashcards

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Biology 101 flashcards
1
Q

Germ Theory

A

Robert Koch

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2
Q

Father of Microbiology

A

Anton Van Leeuwenhoek

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3
Q

HEPA filter pore size

A

0.3 um

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4
Q

Biosafety Level for Bacillus anthracis

A

BSL-2

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5
Q

Biosafety Level for Bacillus subtilis

A

BSL-1

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6
Q

RFLP stands for

A

Restriction Fragment Length Polymorphism

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7
Q

Bacteria’s Site for energy synthesis

A

Plasma Membrane

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8
Q

Extrachomosomal DNA that carries the antibiotic resistant genes

A

Plasmid

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9
Q

Metachromatic granules of Corynebacterium

A

Babes-ernst

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10
Q

For bacterial adherence

A

Common Pili

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11
Q

Mobile, jumping genes (Transfer Gene)

A

Transporon

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12
Q

Tumbling motility

A

Listeria

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13
Q

Gliding motility

A

Capnocytophaga

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14
Q

Darting motility

A

Campylobacter

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15
Q

Corkscrew motility

A

Spirochetes

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16
Q

Twitching motility

A

Kingella

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17
Q

Carbon dioxide requirement for Capnophilic organisms

A

5-10%

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18
Q

Way to differentiate Listeria from Yersinia enterocolitica

A

Gram Stain

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19
Q

Obligate Aerobe

A

Mycobacteria

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20
Q

Obligate anaerobe (2)

A

Veillonella and Actinomyces

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21
Q

Aerotolerant anaerobe

A

Lactobacillus

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22
Q

Classification of PATHOGENIC bacteria based on temperature

A

Mesophilic

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23
Q

Temperature requirement for bacterial growth

A

35 C

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24
Q

Temperature requirement for Virus

A

37 C

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25
Q

pH requirement of pathogenic bacteria

A

7.2 - 7.4

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26
Q

Salt concentration for Staph aureus

A

7.5% NaCl

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27
Q

Salt concentration for E. faecalis

A

6.5% NaCl

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28
Q

Most common blood bag contaminant

A

Yersinia enterocolitica (at 4 C)

Also:

Pseudomonas fluorescens
Serratia liquifacien

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29
Q

End product(s) of Fermentation

A

Acid/Alcohol

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30
Q

Purpose of Burham Tube

A

Gas Detector

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31
Q

Mantoux Test is a skin test for the detection of

A

Tuberculosis

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32
Q

Cotton is toxic to

A

Neisseria

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33
Q

Calcium alginate is toxic to

A

Virus

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34
Q

Specimen to detect acute stage of Weil’s Disease

A

Blood/CSF (Leptospirosis)

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35
Q

CSF transport temperature

A

Room Temp

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36
Q

Urine Presevative

A

Boric Acid

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37
Q

Transport medium for stool pathogens

A

Cary Blair

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38
Q

Transport medium for bacteria and virus

A

Stuart’s

39
Q

Transport medium for respiratory spx

A

Amies

40
Q

Todd Hewitt is for

A

Streptococcus agalactiae

41
Q

Modified Todd Hewitt

A

LIM

42
Q

Preferred swab for virus

A

Dacron

43
Q

CSF bottle and storage temp for micro

A

Bottle 2; RT

44
Q

Ratio of blood:broth

A

1:10

45
Q

Concentration of blood bottle anticoagulant

A

0.25% SPS

46
Q

Formula for colony count in CFU/mL

A

Number of colonies x 1 000

47
Q

Used to assess the quality of sputum

A

Bartlett’s Classification

48
Q

Decontaminant for Sputum spx

A

2-4% NaOH

49
Q

Effect of using acidic iodine as mordant in Gram Stain

A

False Gram (-)

50
Q

Yeasts are Gram ____

A

+

51
Q

All spiral organisms are Gram ___

A

-

52
Q

Primary stain for AFB

A

Carbol-fuchsin

53
Q

Cold AFB staining method

A

Kinyoun Method

54
Q

Hot AFB staining method

A

Ziehl-Neelsen

55
Q

Schaeffer-Fulton is a special stain for

A

Endospores

56
Q

Stain for nucleic acid

A

Acridine orange

57
Q

Extent to which detail of the object is maintained

A

Resolution

58
Q

Closest distance of the the two objects can be distinguished from each other

A

Resolving Power

59
Q

Microscope used to observe viral morphology

A

EM

60
Q

ATCC stands for

A

American Type Culture Collection

61
Q

pH requirement for Culture Media

A

7.2-7.4

62
Q

Biphasic culture medium

A

Castaneda for Brucella blood culture

63
Q

Horse BAP is for

A

Haemophilus spp

64
Q

Human BAP is for

A

Gardnerella vaginalis

65
Q

Sheep BAP is used to differentiate

A

Streptococcus spp.

66
Q

Medium for non fastidious organisms

A

Nutrient Agar

67
Q

Percent of chocolate in CAP

A

0%

68
Q

PEA is for

A

Gram + bacteria

69
Q

Positive result of E. coli in SMAC

A

Colorless

70
Q

Most resistant to sterilization

A

Prions

71
Q

CJD agent

A

Prions

72
Q

Principle of Autoclave

A

Steam under pressure

73
Q

Test for success of Pasteurization

A

Phosphatase Test

74
Q

Cellulose membrane liquid filter pore size

A

0.22 um

75
Q

Best antiseptic

A

Iodine

76
Q

Prevents gonococcal opthalmia neonatorum

A

1% silver nitrate

77
Q

Standard Disinfectant

A

Phenol (Carbolic Acid)

78
Q

QUATS - inactivated by ___

A

Organic materials

79
Q

Serum bactericidal test

A

Schlichter Test

80
Q

Medium for AST

A

MHA

81
Q

Standard depth for Kirby-Bauer Disk Diffusion

A

4 mm

82
Q

Standard antibiotic disc size for Kirby-Bauer Disk Diffusion

A

6mm

83
Q

Standard disc distance for Kirby-Bauer Disk Diffusion

A

15 mm

84
Q

Standard time before disc is applied in Kirby-Bauer Disk Diffusion

A

15 mins

85
Q

Kirby-Bauer Disk Diffusion

Swarming and inner zone should be

A

Ignored

86
Q

General AST media for MRSA

A

MHA + 2% NaCl

87
Q

Kirby-Bauer Disk Diffusion Result

Thick Medium

A

False Resistant

88
Q

Kirby-Bauer Disk Diffusion Result

Increased Drying

A

False Sensitive

89
Q

Drug Resistance Test (+) Result

ESBL

A

Elliptical clearing on Cefotaxime-Clavulanic Acid -Aztreonam disk

90
Q

Drug Resistance Test (+) Result

AmpC beta lactamase test

A

Flattened edge zone on cefoxatime disk

91
Q

Drug Resistance Test (+) Result

Modified Hodge Test

A

Clover-Leaf Like

92
Q

Drug Resistance Test (+) Result

(MBL) Metallo-Beta Lactamase Test

A

Keyhole between imipenem

93
Q

Drug Resistance Test (+) Result

mecA mediated oxacillin resistance

A

Oxacillin and Penicillin Resistant

94
Q

Drug Resistance Test (+) Result

(ICR) Inducible Clindamycin Resistance

A

Flattening (D zone) of clindamycin

Microbiology (9 decks)

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