Gram Negative Bacteria Flashcards
Lactose fermenter
Green sheen
E. coli
Lactose fermenter
Urease positive
ESBL
Klebsiella pneumoniae
Comma shaped
Motile
Oxidase positive
Vibrio cholerae
Comma shaped
Microaerophilic
Skirrows agar
Campylobacter jejuni
Comma shaped
Urease positive
H. pylori
Non lactose fermenting
Motile
Oxisase negative
H2S producer
Salmonella
Non lactose fermenter
Non motile
Oxidase negative
H2S non producer
Shigella
Non lactose fermenter Swarming Oxidase negative H2S producer Urease positive
Proteus mirabilis
Non lactose fermenter
Oxidase positive
H2S non producer
Obligate aerobe
Pseudomonas aeruginosa
Selective media for gram negative bacteria
EMB or MacConkey agar
Lactose fermenters
CKEE
LaCtose is KEE. Grow in MacConKEE agar
Citrobacter
Klebsiella
E coli
Enterobacter
This enterotoxin in E coli causes bloody diarrhea
Verotoxin (Shiga-like)
E coli typing is by the
O and H antigens
O- lipopolysaccharide
H- flagellum
Enterobacteriaceae organisms
MESSY SPECK
Morganella Escherichia Shigella Salmonella Yersinia Serratia Proteus Enterobacter Citrobacter Klebsiella
Most common cause of community acquired/ hospital UTI
Escherichia coli
2nd most common cause of Neonatal meningitis
Escherichia coli
Causes of neonatal meningitis
BEL
B group streptococci
Escherichia coli
Listeria monocytogenes
E.coli strain that causes watery diarrhea
ETEC
Enterotoxigenic
E coli strain that causes prolonged watery diarrhea in infants and in developing countries
EPEC
Enteropathogenic
E coli strain that causes bloody diarrhea and HUS
EHEC
Enterohemorrhagic
E coli strain that causes bloody diarrhea without HUS
EIEC
Enteroinvasive
Symptoms of HUS
Hemolysis
Uremia
Thrombocytopenia
E coli strain that causes persistent watery diarrhea in HIV patients
EAEC
Enteroadherent
Treatment for E coli UTI
Ampicillin
Sulfonamides
Treatment for E coli meningitis
3rd generation cephalosporins
To which serotype does EHEC belong?
E coli O157: H7
Toxin mediated mechanism for EHEC
Inhibition of 60s ribosomal subunit that causes decreased protein sysnthesis in gastrointestinal cells
Gram negative diplococci encapsulated, ferments GLUCOSE AND MALTOSE
Kidney bean shaped oxidase positive
Neisseria MeninGitidis
Gram negative diplococci, ferments GLUCOSE ONLY
Neisseria gonorrhea
Most common cause of meningitis among 2-18 years old
N meningitidis
Most severe form of meningococcrmia where there is widespread purpura, high fever, shock, DIC and adrenal insufficiency
Waterhouse -Friderichsen syndrome
Treatment of meningococcemia
Penicillin G
Chemoprophylaxis for meningococcemia
Rifampin
Vaccine for meningococcemia include what strains
A
C
Y
W135
Thayer Martin agar contains which antibiotics?
Vancomycin
Colistin
Nystatin
Usual coinfection of Neisseria gonorrhea
Chlamydia trachomatis
Most common cause of urethritis and PID
N. gonorrhea
Complication of PID where there is perihepatitis with violin string adhesions
Fitz Hugh Curtid syndrome
Most common cause of septic arthritis in sexually active adults
N. gonorrhea
Most common cause of septic arthritis in general
Staphylococcus
Treatment of N. gonorrhea
Ceftriaxone and doxycycline due to frequent co infection with chlamydia
This bacteria produces satellite phenomenon around S.aureus colonies
H influenzae
Hib infections affects children 6mos -1 year old due to
Decline in maternal IgG and immature immune system
Most common cause of epiglottitis
H influenza b
Epiglottitis on xray
Thumb sign
Treatment of epiglottitis
Ceftriaxone
Toxin that causes whooping cough in pertussis that damages ciliated cells
Tracheal cytotoxin
Stage in pertussis where the start of whoops occur and with vomiting, 2-4 weeks into the illness
Paroxysmal stage
Treatment of pertussis
Erythromycin
Facultative intracellular bacteria
Some Bugs May Live FacultativeLY Salmonella Brucella Mycobacteria Legionella Francisella Listeria Yersinia
Sole virulence factor of Legionella
Endotoxin
Treatment for legionella
Azith or erythromycin
Test to detect antibodies to salmonella in patient’s serum
Widal test
Salmonella typhi has a predilection for invasion of what organ
Gallbladder
Typhoid fever is caused by the presence of ______ in salmonella
Vi (virulence) capsular antigen
Salmonella typhi multiplies in the
Peyers patches
Culture sources for salmonella typhi
BUS
Week 1- blood
Week 2- urine
Week 3- stool
Treatment for salmonella
Cipro
Philippines: amox chloramphenicol, cotrimoxazole
Facultative gram negative rods that produce H2S
Salmonella spp
Shigella transmission
4 F
Food fingers feces flies
Shigella preferentially invades this part of the intestines
Distal ileum and colon
Most common cause of bacillary dysentery
Shigella sonnei (Duval bacillus)
Most severe form of bacillary dysentery
Most common cause of epidemic dysentery
Shigella dysenteriae type 1
Shiga bacillus
Shigella dysentery frequently resolves in
2-3 days
Shigella is treated with ____ in severe cases
Ciprofloxacin
Gram negative comma shaped rods with shooting star motility
TCBS agar
Vibrio spp
Gram negative bacteria that can be acquired through eating contaminated seafood or sea water
Vibrio parahaemolyticus and vulnificus
Rice water stools
Massive dehydration
Washerwoman hands
Cholera
Treatment of cholera
Tetracycline
Treatment of vibrio parahaemolyticus or vulnificus
Minocycline
Microaerophilic comma shaped gram negative rods
Oxidase and catalase positive
Grows 42C on Skirrows agar
Campylobacter jejuni
Most common cause of bacterial gastroenteritis
Campylobacter jejuni
Campylobacter jejuni commonly damages this part of the colon and may mimic what disease
Jejunum
Ulcerative colitis
Campylobacter jejuni may also be associated with these diseases
GBS
Reiters syndrome
Triad of Reiter syndrome
Cant pee
Cant see
Cant climb a tree
Urethritis
Uveitis
Arthritis
Treatment of campylobacter jejuni
Symptomatic
Erythromycin for severe cases
Microaerophilic gram negative comma shaped rods, urease positive that neutralizes stomach acid
Helicobacter pylori
Urease positive bacteria
Particular Kinds Have Urease
Proteus
Klebsiella
H pylori
Ureaplasma urealyticum
Facultative gram negative rods with large polysaccharide capsule
Urease positive
Klebsiella pneumonia
Most common cause of pneumonia in alcoholics
Klebsiella
Characteristic sputum of Klebsiella pneumonia
Currant jelly
Facultative gram negative rods non lactose fermenting urease positive
Swarming motility
Proteus mirabilis
Proteus infection has urease that predisposes the formation of
Struvite stones
And staghorn calculi
Struvite stones are composed of
Magnesium
Ammonium
Phosphate
Treatment of proteus mirabilis infection
TMP SMX
ampicillin/ quinoles
Obligate aerobe gram negative rod with sweet grape like odor
Pseudomonas aeruginosa
Hot tub folliculitis is caused by
Pseudomonas
Most common cause of malignant otitis media in diabetics
Pseudomonas
3rd most common cause of nosocomial UTI
Pseudomonas
Burn wound infection
Ecthyma gangrenosum
Febrile neutropenia
Shanghai fever
Pseudomonas
Possible combinations of antibiotics for pseudomonas
Ceftazidime and amik
Piperacillin and amik
Rifampicin for refractory cases
Anaerobic gram negative rods
Predominant anaerobe of human colon
Spreads during bowel trauma or surgery
Can cause abdominal abscess
Bacteroides fragilis
Treatment for bacteroides fragilis
Metro