Gram - Bacteria Flashcards
Gram -, Diploccoci. IgA protease + Maltose fermenter, Polysaccharide capsule
Neisseria meningitidis
Gram -, Diplococci. IgA protease + ,Maltose non-fermenter. No capsule
Neisseria gonorrhea
Gram - coccobacillus. Grows of Chocolate Agar with Factor V and X
Haemophilus influenzae
Note: S.aureus can produce factor V which allows Haemophilus to grow
Gram - coccobacillus. Seen in animal bites.
Pasturella
Gram - coccobacillus. Toxin inhibits Gi leading to decreased phagocytic ability. Leads to coughing on exhalation and characteristic sound upon inspiration in children
Bortadella pertussis
Gram -, Comma shaped. Oxidase + and grows on Alkaline media. Toxin increases cAMP leading to Cl- efflux and multiple ‘rice water stools’
Vibrio Cholera
Gram -, Comma shaped. Oxidase + . Causes bloody diarrhea and is often transmitted by c fecal oral route through foods. Grows at 42 deg.
Campylobacter jejuni
Gram- Rod, Fast Lactose fermenter. Encapsulated , indole -
Klebsiella
Gram- Rod, Fast Lactose fermenter. Encapsulated , indole +, Catalase +
E.coli
Gram- Rod, Fast Lactose fermenter. Not E.coli or Kleb haha
EnteroBACTER (not enterococcus, which is GROUP D STREP)
Gram- Rod, Slow Lactose fermenter. Catalase +
Serratia
Gram - Rod, Lactose non-fermenter , Oxidase - . Cell to cell transfer. Bloody Diarrhea Produces a toxin which inactivates the 60s ribosome.
Shigella
Gram - Rod, Lactose non-fermenter , Oxidase -. Urease +
Proteus
Gram - Rod, Lactose non-fermenter , Oxidase -. H2S+, Disseminated hematogenously. BLoody diarrhea. Often passed via uncooked poultry
Salmonella
Gram - Rod, Lactose non-fermenter , Oxidase +, Catalase + Blue Green pigmentation, grape like odor
Pseudomonas aeruginosa
Gram - Rod, Lactose non-fermenter , Oxidase +, Urease + . Involved in gastric ulcer formation.
H.pylori
Grow Pink colonies on MacConkey Agar
Lactose Fermenters !!
LaCtoSe is KEE
Klebsiella
E.coli
Enterobacter
Citrobacter
Serratia
What drugs are most Gram -‘s resistant to ?
Penicillin G and Vanco (due to outer membrane)
May still be susceptible to Amoxicillin and Ampicillin
What disease are associated with N.gonorrheae
Gonorrhea
PID
FItz-Hugh Curtis
Septic Arthritis
What disease are associated with N. meningitides ?
Meningitis
Meningococcemia
Waterhouse Friedrichson (Adrenal infarction)
What is the treatment of choice for Neisseria gonorrhea
Ceftriaxone
May treat chlamydia infection concurrently with Azithromycin or Doxy.
Prophylaxis for N. menegitidis
Definitive treatment
Rifampin, Cipro, Ceftriaxone
Ceftriaxone and Penicillin G
What illnesses does Haemophilus influenzae cause ?
haEMOPhilus
Epiglotitis
Meningitis (Encapsulated)
Otitis media
Pneumonia (think influenza)
Describe the composition of the Vaccine for Haemophilus influenzae
Type B capsular Polysaccharide complexed to Diptheria toxoid.
Gram -, Rod (really doesn’t gram stain) . Grows on BCYE with iron and cysteine. Visualized with Silver Stain. Aerial transmission in droplets
Legionella
How do you treat Legionella
Macrolide or Quinolone
Illnesses caused by Psuedomonas
PSUEDO F !
Pneumonia Sepsis External otitis UTI Diabetic Osteomyelitis Folliculitis
Like Diptheria toxin, Pseudomonas produces a toxin (ExoToxin A) which ….
Inhibits EF2 –> lack of protein synthesis
Treatment for pseudomonas
Aminoglycoside
Extended Spectrum penecillin
Due to expression of Fimbriae. E.coli have the ability to cause …
UTI (Cystitis and Pyelonephritis)
What does the K-capsule of E.coli allow it to do ?
pneumonia !
In which patients does Klebsiella (Gram - , Lactose + , ) cause lobar pneumonia preferentially ?
Alcoholics and Diabetics (when aspirated)
Buzz word for Klebsiella infection
4 A’s
Red- Currant Jelly !
Aspiration
Abscess
Alcoholics
diAbetics
Signs and symptoms of Salmonella typhi (Typhoid fever) .
Rose spots on abdomen
Diarrhea
Fever
Headache
Resides in gallbladder in carrier state.
Campylobacter often pre-cedes what two illness ?
Guillain Barre
Reactive Arthritis
This gram - organism can cause symptoms similar to Crohns or appendicitis and is transmitted via pet feces, contaminated milk or pork.
Yersinia enterolitica
Besides peptic ulcer disease, what can H.pylori cause
Adenocarcinoma
Lymphoma
How would you treat H.pylori
Triple therapy
PPI
Clarithromycin
Amoxicillin or metronidizole
Spirochete that can be visualized by Giemsa stain
Borrelia
Leptospira and Treponema are t0o small and are usually diagnosed by dark field or IF.
This spirochete is found in water contaminated with animal urine. Causes jaundice, photophobia and conjunctivitis. SURFERS IN HAWAII
Leptospira Interrogans
Severe form of Leptospira infection
Weil Disease : Jaundice, azotemia, fever hemorrhage and anemia.
What organism causes Lyme disease ?
Borrelia burgdorferi (Spirochete, giemsa stain +)
What is the natural reservoir for Borrelia ?
MICE (not deer!)
Stage I of Lymes disase
Bulls Eye Rash (Erythema Chronicum Migrans)
Stage II Lymes Disease
Neuorlogic (facial palsy)
Cardiac Issues
Stage III Lymes Disease
Musculoskeletal and neurologic (encephalopathy and polyneuropathy)
Monoarthritis
Lyme Disease Syptoms: FACE
Facial nerve palsy
Arthritis
Cardiac block
Erythema migrans
Syphilis is caused by
Treponema pallidum
Primary Syphilis
PAINLESS Chancre on genitals (Treponema present)
Secondary Syphilis
Copper colored maculopapular rash on entirety of body (Palms and Soles)
Condyloma Lata (Wart like lesion on the genitals, Treponema present ),
Tertiary Syphilis
Gummas
Aortitis
Neurosyphilis (Tabes Dorsalis, argyle robertson pupil )
Congenital Syphilis
Saber Shins Hutchinson teeth Saddle nose CN VIII Deafness Mulberry molar
How do you screen for syphilis ?
VDRL
Why must you confirm +VDRL syphilis test with FTA-ABS (Immunofluorescence) ?
VDRL has many False Positive
VIruses (mono, hepatitis)
Drugs
Rheumatic Fever
Lupus***, Lerposy
What is the treatment for Syphilis ?
Penicillin G
What causes the Jarisch Herxheimer Rxn ?
Release of pyrogens after spirochetes destroyed in syphilis
Vector: Bartonella
Cat scratch fever (Cats)
Vector: Borrelia reccurentis
Reccurent fever (from Louse)
Vector:Campylobacter (zoonoses)
Puppies, livestock (fecal oral0
Vector:Chlamydophila psittaci
Parrots and other idiot birds
Vector:Coxiella burnetti
Q fever . Cattle Sheep amniotic Fluid
Vector:Ehrlichia chaffeensis
Lone star tick (Ehrlichiosis
Vector:Francisella tularensis
Rabbits (tularemia)
Vector:Leptospirosis (leptospira interrogans)
ANimal piss (Weil Disease, learn it fool)
Vector:Lerposy (in the US)
Armadillos
Vector: Pasturella multicoccida
Animal bites (dog/cat)
Vector:Rickettsia prowazecki
Epidemic typhus (louse)
Vector: Ricketsiaa ricketsii
RMSF (tick)
Vector: Rickettsia typhi
Endmic typhus (Flea)
Yersina pestis
black plague haha (flea)
Gram variable rod, causitive agent of Bacterial Vaginosis . “Clue Cell” , fishy odor (non-painful).
Gardnerella vaginalis
Gardnerella vaginalis treatment
Metronidazole
Treatment for all Rickettsial Diseases
Doxycycline (REGARDLESS OF AGE, more important than bone, Cardiac Damage can kill you)
Centripital rash, starting at wrist and going to trunk. Cover palms and soles. SE United States, out in the woods.
Rocky Mountain Spotted Fever
Rickettsial rash beginning on Trunk and moving out in centrifugal manner
Typhus (Rickettsia prowazecki or Rickettsia Typhi)
What is present in the cytoplasm of monocytes infected with Ehrlichia ?
Morula (NO RASH IS SEEN)
Which is the infectious form of Chlamydia: Elementary or Reticulate body ?
Elementary (enters via endocytosis. Chlamydia are obligate intracellular organisms)
Reticulate body is for replication
Treatment for all Chlamydia
Azithromycin (one time treatment)
Can give doxy too
Chlamydia Trachomatis Type A,B,C
African Conjunctivitis, Blindness (conjunctivitis), Chronic infection
Chlamydia Trachomatis Type D-K
Urethritis, PID , Neonatal pneumonia
Chlamydia Trachomatis Type L1,L2,L3
Lymphogranuloma venerum : LGV may begin as a self-limited PAINLESS genital ulcer that occurs at the contact site 3–12 days after infection…Lymphadenopathy and buboes appear
What disease is caused by Mycoplama pneumoniae ?
Walking Pneumonia
Non-productive cough, sweating, diffuse interstitial infiltrate
Mycoplama pneumoniae diagnosis
High titer of IgM cold Agglutinins
Growth on Eatons Agar
X-ray with interstitial infiltrate beyond presentation of patient illness
What is found in the cell membrane of Mycoplama pneumoniae ?
Sterols (for support since they do not have cell walls)
What is the treatment for Mycoplama pneumoniae ?
Macrolide of Fluoroquinolones (penicillins will not work since there is no cell wall)