GMO Flashcards
1
Q
how are GMos generated
A
generated through application of recombinant DNA tech and gene transfer tech GMO
2
Q
GNO detections
A
are on protein or genomic levels
3
Q
what does PCR in GMO detection requires
A
- nucleotides ( A, T, G, C)
- taq DNA polymerase
- DNA primers
- DNA template ( cDNA, genomic DNA, plasmid DNA)
4
Q
method od detectin GMO
A
add 4 requirements
heat DNA to separate double helix
double the population by amplifying
Amplify the modified region to increase the conc of species
run DNA through electrophoresis ( will run based on mol weight) - larger slower migration will sty at the top
use agarose gel to slow down the movement and separate by size high mol weight mol
5
Q
what are 2 types of sequences available
A
the maxam- Gilbert method ( chemical method)
the sanger dideoxy methos ( chain termination method )
