GM vaccine Flashcards

1
Q

how many people suffer from diseases which could be preventable via vaccination

A

2 billion

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2
Q

what does current research focus on

A
DNA plasmids
vectors
transgenic plants
viruses
non pathogenic organisms
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3
Q

antibiotics have no

A

immunological memory

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4
Q

antibiotics increase the risk of

A

resistant strains of pathogen

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5
Q

antibiotics do not work against

A

viruses

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6
Q

how can we prevent the disease

A

vaccination

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7
Q

define vaccination

A

exposing a person to material that is antigen but not pathogenic

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8
Q

currently have vaccinations for many diseases such as

A
TB - BCG
chicken pox
measles
diphtheria
yellow fever
polio
mumps
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9
Q

what was the mortality rate of small pox

A

30%

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10
Q

who performed first experimental vaccination

A

edward jenner

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11
Q

when was small pox virus deemed irradiated

A

1977

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12
Q

how does a vaccine wotk

A

stimulates immune system via antibodies

creates memory cells so antibodies are quicker next time

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13
Q

what is the 2 pronged attack

A

helper T and B lymphocyte

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14
Q

what type of a response is B lymphocyte

A

humoral - attack outside cells

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15
Q

what do killer T cells do

A

attack infected cells and pathogen inside

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16
Q

when does proliferation of B cells occur

A

primary response when first infected

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17
Q

how long is required for the B cells to generate the maximum effector cell response

A

10-17 days

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18
Q

if body is exposed to an antigen again later how long does it take for B cells to generate the maximum effector response

A

2-7 days

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19
Q

what is secondary exposure known as

A

secondary response

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20
Q

what makes a vaccine safe

A

doesn’t cause illness or death

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21
Q

what makes a vaccine protective

A

protects against illness resulting from exposure from pathogen

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22
Q

how long should the protection from a vaccine last

A

at least few years

23
Q

what are practical considerations for vaccines

A

low cost
biologically stable
ease of administration
few side effects

24
Q

what does polo vaccine need and why

A

neutralising antibody as polio infects neutrons which cannot be replaced

25
Q

name the 2 types of vaccine

A

killed inactivated and live attenuated

26
Q

describe killed vaccie

A

don’t develop disease

27
Q

name killed vaccine

A

hep A
polio
whooping cough

28
Q

are killed vaccines safer than live ones

A

yes

29
Q

when microbes are killed, what must it not alter

A

the antigens responsible for stimulating immunity

30
Q

which response do killed vaccines create

A

humoral - don’t usually get T killer involved

31
Q

what are the problems with killer vaccines

A

only produce humeral
not effective against pathogens that infiltrate cells
need booster as effect wares off

32
Q

describe live attenuated vaccine

A

a process which lessens the virulence of the microbe

33
Q

what is a live attenuated vaccine used for

A

viruses, such as measles, mumps, rubella

34
Q

how does the live attenuated vaccine work

A

multiply like normal organism,
activated all phases of immune system
raises immune response for all protective antigens
quick immunity

35
Q

advantage of live attenuated

A

quick immunity
spread to contact of vaccinee even if they haven’t connected (heard immunity)

no need for booster

36
Q

disadvantages of live vaccine

A

mutation - reversion to virulence
spread to contacts who haven’t given concent
problem in immunodeficiency disease and pregnancy

37
Q

what is a recombinent live attenuated vaccine

A

modification of pathogen - e.g.. delete virulence genes and leave others which gives immune response

38
Q

give an advantage of recombinant live attenuated vaccine

A

can add other immunity inducing genes from other diseases to produce polyvalent vaccine

39
Q

what is a subunit vaccine

A

use antigenic fragments which best stimulate immune response
contain specific subunit of protein

40
Q

disadvantages of subunit vaccine

A

less effective then whole agent vaccines - adjuvants usually needed

costly
always require boosters

41
Q

advantages of subunit vaccine

A

single antigen

safer as cannot reproduce

42
Q

which specific protein subunit is used in subunit vaccines

A

usually protein coats or modified toxin

43
Q

what is a DNA vaccine

A

DNA sequence used as the vaccine

44
Q

how does DNA vaccine work

A

put DNA sequence for antigenic protein pathogen into cells and is translated to form antigenic protein
this is foreign to cells so immune response triggered

45
Q

advantages of DNA vaccine

A

uses only DNA from infectious organism

avoid risk of using actual organism
provide both humeral and cell mediated immunity
refrigeration is not required

46
Q

disadvantages of DNA vaccine

A
insertional mutagenesis
chromosome instability
turn on oncogenes
turn off tutor suppression genes
antibodies produced against antigen can cause autoimmune disease
47
Q

DNA vaccines are being developed against —

A

rabies
malaria
aids

48
Q

when were edible vaccines first tested on humans

A

1997 - antidiahroal potato

49
Q

advantages of edible vaccine

A

direct contact with mucosal lining
good for third world
injections dont stimulate immunity very well

50
Q

problems with edible vaccine

A

amount made by plant is usually very low - need to make sure its enough to stimulate immune response

plants grow poorly when producing loads of vaccine

51
Q

what is the best edible thing for the vaccine

A

potato
- stored long period without refrigeration

some countries eaten raw
and heat doesn’t denature protein

52
Q

disadvantage of using potato

A

need to be cooked usually might denature

53
Q

other good examples of edible vaccine things

A

banana

tomato