Glycolysis, TCA, ETC (Common Catabolic Pathways)

Question 1

What is the end product of glycolysis?

Answer 1

Pyruvate

2 pyruvate

then, depending if aerobic or anaerobic, pyruvate will be converted to lactic acid or acetyl CoA

Question 2

Glycolysis: Identify the enzyme

attaches phosphate to glucose, making it glucose-6-phosphate

Answer 2

Glucokinase

or Hexokinase (if in muscles); Glucokinase in liver

Question 3

Glycolysis: Identify the enzyme

Glucose-6-phosphate to Fructose-6-phosphate

Answer 3

Phosphohexose isomerase

remember na Fructose is an isomer of Glucose

Question 4

Glycolysis: Identify the enzyme

Adds phosphate to the Carbon-1 of Fructose-6-phosphate

Answer 4

Phosphofructokinase-1

Question 5

Glycolysis: Identify the enzyme

Breaking down of Fructose-1,6-Bisphosphate into Dihydroxyacetone phosphate and Glyceraldehyde-3-Phosphate

Answer 5

Aldolase

Question 6

Glycolysis: Identify the enzyme

Dihydroxyacetone phosphate to Glyceraldehyde-3-phosphate

Answer 6

Phosphotriose isomerase

Question 7

Glycolysis: Identify the enzyme

Glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate

Answer 7

Glyceraldehyde-3-phosphate Dehydrogenase

Question 8

Glycolysis: Identify the enzyme

1,3-Bisphosphoglycerate to 3-phosphoglycerate

Answer 8

Phosphoglycerate kinase

Question 9

Glycolysis: Identify the enzyme

3-phosphoglycerate to 2-phosphoglycerate

Answer 9

Phosphoglycerate mutase

Question 10

Glycolysis: Identify the enzyme

2-phosphoglycerate to enol (Phosphoenol pyruvate)

Answer 10

Enolase

Question 11

Glycolysis: Identify the enzyme

Phosphoenol pyruvate to pyruvate

Answer 11

Pyruvate kinase

Question 12

Glycolysis

In what step can you find the subtraction of ATP?

like what enzyme and/or products nangyari

Answer 12

In the hexokinase & phosphofructokinase-1 step

(basta kinase, involved phosphates diyan)

• glucose to glucose-6-phosphate; fructose-6-phosphate to fructose-1,6-bisphosphate
• tig-1 ATP so minus 2 the gross total

Question 13

Glycolysis

In what steps can you find the addition of 2 ATP?

like what enzyme and/or products

Answer 13

Phosphoglycerate kinase & Pyruvate kinase step

• 1,3-Bisphosphoglycerate to 3-Phosphoglycerate; Phosphoenol pyruvate to Pyruvate

Question 14

Glycolysis

In what steps can you find the reduction of NAD⁺ to NADH?

Answer 14

Glyceraldehyde-3-phosphate Dehydrogenase

Question 15

In what part of the cell does glycolysis occur?

Answer 15

Cytoplasm

Question 16

Glycolysis: Identify the enzyme class

Hexokinase & Glucokinase

Answer 16

EC2

Transferase

Question 17

Glycolysis: Identify the enzyme class

Phosphohexose isomerase

Answer 17

EC5

Isomerase

Question 18

Glycolysis: Identify the enzyme class

Phosphofructokinase-1

Answer 18

EC2

Transferase

Question 19

Glycolysis: Identify the enzyme class

Aldolase

Answer 19

EC4

Lyase

Question 20

Glycolysis: Identify the enzyme class

Glyceraldehyde-3-phosphate Dehydrogenase

Answer 20

EC1

Oxidoreductase

Question 21

Glycolysis: Identify the enzyme class

Phosphoglycerate kinase

Answer 21

EC2

Transferase

Question 22

Glycolysis: Identify the enzyme class

Phosphoglycerate mutase

Answer 22

EC5

Isomerase

Question 23

Glycolysis: Identify the enzyme class

Enolase

Answer 23

EC4

Lyase

Question 24

Glycolysis: Identify the enzyme class

Pyruvate kinase

Answer 24

EC2

Transferase

Question 25

Glycolysis: Identify the enzyme class

Lactate dehydrogenase

Answer 25

EC1

Oxidoreductase

Question 26

Transition step: Identify the enzyme class

Pyruvate Dehydrogenase

Answer 26

EC1

Oxidoreductase

Question 27

Transition step

What is the end product of the transition (preparatory stage) step b4 kreb’s?

Answer 27

Acetyl CoA

2 Acetyl CoA since 2 pyruvate produced

Question 28

Transition step

Considering the pyruvate of 3 carbons and the Acetyl-CoA that has 2 carbons, what are the products produced in this step?

NADH, CO₂

Answer 28

• 2 Acetyl CoA
• 2 NADH
• 2 CO₂

CO₂ is der coz carbon is removed (via pyruvate dehydrogenase)