glycolysis Flashcards
order of the products of glycolysis
glucose 6 phosphate
fructose 6 phosphate
fructose 1,6 bisphosphate
glyceraldehyde 3 phosphate (G3P)
1,3 bisphosphosphoglycerate
3 phosphoglycerate
2 phosphoglycerate
PEP
Pyruvate
glycolysis occurs in the
cytoplasm
3 phases of glycolysis
energy investment phase, cleavage, energy payoff phase
during the energy investment phase, the cell spends
atp
this investment is paid off with interest in the energy payoff phase. when ATP is produced by
substrate level phosphorylation
net energy from glycolysis
2 ATP, 2 NADH
this enzyme transfers a phosphate group from ATP to the sixth carbon of glucose, investing the first ATP
hexokinase
the resulting product of glucose 6 phosphate is converted into its isomer fructose 6 phosphate with the enzyme
phosphogluco-isomerase
this enzyme transfers a phosphate group from ATP to the first carbon of sugar. this is the second ATP invested. the resulting product will become fructose 1,6-bisphosphate
phosphofructoskinase-1 (PFK-1)
this enzyme cleaves the six carbon sugar fructose 1,6 bisphosphate into two different sugars.
aldolase
what are the two sugars produced during the cleavage
Dihydroxyacetone (DHAP) and Glyceraldehyde 3-phosphate (G3P)
the conversion from DHAP to G3P happens with the enzyme
triose phosphate isomerase
the two G3P molecules from step 4 and 5 enter the
energy payoff phase.
In step 6, this enzyme will catalyze two sequential reactions.
First, the sugar is oxidized by the transfer of electrons to NAD+ forming NADH.
and second, a phosphate group is attached to the oxidize substrate making a high energy product 1,3-bisphosphoglycerate
triose phosphate dehydrogenase
this enzyme transfers a phosphate group from 1,3-bisphosphoglycerate to ADP to produce ATP and 3-phosphoglycerate
phosphoglycerokinase