BIO Q2 LQ2 Flashcards
The technical application of biological knowledge for human purposes
Biotechnology
Biotechnology focuses on using living organisms like
bacteria, viruses, and fungi
Mainly used to create vaccines
Bio organisms
mainly used to create GMO Fruits and Vegetables
Plants
Mainly used to make better quality of products
Animals
Manipulation of the genetic makeup of cells or whole organisms
Genetic engineering
Universal language in biotechnology. It is present in all species.
ACTG
Cutting, splicing, and copying DNA
→ aims to amplify genetic material by combining two DNA sequences. It falls under genetic engineering.
Recombinant DNA Technology
How long is a cycle of Recombinant DNA Technology
24 hours
This is an example of _____: The process involves merging two segments of DNA strands. The first strand contains the desired DNA, such as genes from a lizard that enable wall-climbing. This desired DNA is combined with a second DNA strand from bacteria, chosen for its rapid reproduction rate. To perform this, tools are needed for cutting and pasting.
→ The lizard gene and bacterial DNA are fragmented and then joined together. As the bacteria reproduces, the desired gene multiplies. Eventually, the gene is harvested.
Recombinant DNA Technology
Human DNA is
Helical
Bacteria DNA is
Circular
Recombinant DNA technology process
- Cutting DNA into fragments
- Combining them
- Reintroducing them into
bacteria - Incubating
- Extracting and harvesting the gene
cut DNA at specific sites, often
palindromes
Restriction enzyme
Join fragments of DNA
DNA ligase
→ are strings of letters that read the same forwards and backwards.
→ They are significant because they act as signals for restriction enzymes. When these enzymes encounter palindromic sequences, they are prompted to initiate a cutting process.
Palindromic sequences
→ Cooling down the DNA sample
→ the reaction to 55-65°C for 45 seconds so primers can bind to their complementary sequences on the single-stranded template DNA
→ Allows the primer to attach to the DNA strand
→ Duration: 45 seconds
Annealing
→ Heating the reaction temperature
to 72°C for 2 minutes so Taq polymerase extends the primers, synthesizing new strands of DNA
→ Subject the DNA sample to heat again
→ Prepares for replication and the creation of complementary sister strands
→ A polymerase is added to the solution to facilitate extension
Extension
shares the same objective as recombinant DNA technology: amplifying genetic material. However, this method employs machines for a faster process, often used in medical settings for safety.
Polymerase chain reaction
How long does a cycle of PCR last?
5 minutes
PCR Cycle
- Denaturation
- Annealing
- Extension
- Cooling repeat cycle
→ Heating the reaction strongly (96°C for 1 minute) to separate, or denature, the DNA strands. This provides the single-stranded template for the next step
→ Subject the DNA to heat
→ Heat allows the hydrogen bonds to
separate, resulting in the separation of DNA strands 1 and 2
→ Duration:1minute
denaturation
Final temperature of amplicon solution should be cooling back to 5°C, this is to ensure proper bonding of newly synthesized strands of DNA
→ Cooling it down to stabilize everything
Cooling repeat cycle
→ Prior to sequencing, DNA must be amplified to get adequate amount
→ A,G,C,T fluorescent-labeled
dideoxynucleotides
→ Step1: Amplification, then Step2: Sequencing
→ Amplifying the DNA strands to
increase the amount available for sequencing
→ Sequencing involves examining the sequence of nucleotides in the DNA strands, which is not feasible without amplification
→ Colorimetric method: Utilizing color changes to measure the sequencing results
→ The first result shows bonds. Horizontal bonds are arranged vertically, and it is necessary to transcribe them.
→ The second result involves spikes, with colors used as references to sequence the DNA.
DNA Sequencing
replicates the DNA
DNA polymerase
- enable initiation of DNA
synthesis - A,G,C,T nucleotides
Primers
separates DNA strands by size
Gel electrophoresis
→ Often DNA samples must be amplified by PCR first
→ DNA is cut with restriction enzymes
→ Different individuals will have different lengths of DNA between the restriction sites
→ DNA is separated by gel electrophoresis and fragment pattern is compared
→ Relies on the parallelism of DNA bonds to identify individuals
DNA Fingerprinting
→ Gel is placed in a plastic mold with
electrodes to determine positive and negative sides
→ DNA samples are placed in wells,
typically on the negative side since
DNA is negatively charged
→ DNA migrates from the negative to positive side under the influence of
electricity, creating distinct bands
→ Differential migration occurs due to
variations in nucleotide weight; larger molecules migrate less distance, while smaller ones travel farther
Gel electrophoresis
are developed with the intention of improving human health and addressing various challenges in agriculture and food production.
GMOs
They are created by altering the genetic makeup of organisms, including plants and animals.
GMOs
→ Are normally used because they reproduce rapidly and efficiently.
→ They offer advantages in terms of cost, time, effort, and production
location.
→ have been developed to produce the following:
⧫ Insulin
⧫ Human growth hormone
⧫ Tissue plasminogen
activator (tPA)
⧫ Factor VIII (blood clotting
factor needed by many
hemophiliacs)
⧫ Vaccines
Transgenic bacteria
→ are becoming increasingly popular
and are commonly found in grocery stores.
→ While we often associate GMO plants with physical characteristics such as size, quality, and appearance, it’s important to note that it also enhances other traits like resistance to pests and diseases, and increased vitamin content.
Transgenic plants
Have been modified to express the following:
⧫ Increased resistance to freezing
⧫ Longer shelf life
⧫ Increased vitamin A
⧫ Human proteins (i.e.,
albumin)
⧫ Increased resistance to
pests
Transgenic plants
→ are bigger challenges
→ More difficult to introduce foreign
DNA into animal cells
→ Cloning more difficult
Transgenic animals
used to promote faster animal growth
Bovine Growth Hormone
introducing human genes into dairy animals in such a way that the human protein is produced in the dairy animal’s milk
Gene pharming
These genetically modified pigs are designed to be resistant to the
PRRS (Porcine respiratory and reproductive syndrome)
involves manipulating certain genetic sequences, but its primary goal is not always to enhance physical traits or confer resistance. Instead, its main objective is to treat or correct specific diseases or disorders by targeting the underlying genetic causes or regulating symptoms.
Gene therapy
is a condition where the body lacks the enzyme adenosine deaminase (ADA), a vital immune system enzyme. patients are highly susceptible to infections, and researchers are exploring genetic modification techniques to introduce functional ADA genes into their cells. This will restore ADA’s activity, enabling the production of B and T cells, enhancing the immune system’s defenses and improving the patient’s quality of life.
SCID (Severe Combined Immunodeficiency)
Is a condition characterized by the over-accumulation of mucus, leading to blockages in the airways. Gene therapy has developed nasal sprays to address this issue, providing relief from mucus buildup when needed.
Cystic Fibrosis
People with SCID lack this enzyme
Adenosine deaminase (ADA)
In cancer treatment, gene therapy aims to modify _____ within the patient’s genes. These regulate cell growth, helping to inhibit or regulate the overproduction of cancer cells rather than directly killing them.
Interleukins
Membrane proteins that boost the infection rate.
M
____ is not for killing but coexisting
Vaccination
Contains the nucleus of the genetic material
N
Proteins involved in the assembly and release of mature viruses.
E
Human genes can be packaged in ____, which can introduce genes into human cells
Retroviruses
Spikes, which are attracted to receptors on human cells found in airways.
S
contain the genetic material of COVID, which helps your body build immunity against the virus. This allows your body to remember the virus and respond more effectively if you encounter it again, potentially reducing the severity of the infection. The genetic material in these vaccines can be either DNA or RNA.
Genetic vaccines
is used to amplify DNA, but COVID contains RNA instead. In this process, the RNA strand is converted into a DNA strand through a reversal process.
RT PCR
contain the entire virus, but it’s been killed or inactivated before being used in the vaccine. This allows your body to recognize the virus and build immune memory against it, without causing illness.
Inactivated vaccine
provide specific proteins from the virus to trigger an immune response without introducing the whole virus.
Protein vaccines
contain a weakened form of the virus that can still trigger an immune response but are not strong enough to cause illness.
Attenuated vaccines
Any poisonous chemical substance and its by-products or organism present in our immediate environment that negatively affects human health
- The chemicals of most concern are those that have very low solubilities in water but are highly soluble in fat.
Environmental toxins
Heavy metals directly contaminate soil, leading to immediate exposure risks. Sources: Similar to indirect pollution but directly affecting soil through industrial and domestic activities.
Direct pollution
Concentration: (Increases/decreases) along the food chain, with higher concentrations in organisms higher on the chain.
Increases
naturally occurring substances that accumulate and cause damage to the environment and living beings, including humans
include substances known as semimetals or metalloids that can have the same deleterious effects
Heavy metals
Common heavy metals
arsenic, lead, mercury