Glycolysis Flashcards

1
Q

where does glycolysis occur

A

the cytosol

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2
Q

phosphorylation of glucose (glycolysis step 1) enzyme involved

A

hexokinase

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3
Q

product of first step of glycolysis (phosphorylation of glucose)

A

glucose-6-phosphate

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4
Q

Conversion of glucose-6-phosphate (G-6-P) to ??? (glycolysis step 2) PRODUCT

A

fructose 6-phosphate

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5
Q

step 3 of glycolysis: phosphorylation of F6P. Enzyme involved

A

phosphofruktokinase-1

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6
Q

product of glycolysis step 3

A

fructose 1,6-bisphosphate

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7
Q

glycolysis step 4: f1,6bp cleaves ??? into ??? and ???

A

cleavage of F1,6BP into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate

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8
Q

Glycolysis step 5: Interconversion of the triose phosphates from ??? to ??? (To make 1,3-phosphoglycerate in step 6)

A

dihydroxyacetone to glyceraldehyde-3-phosphate

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9
Q

step 6: oxidation of ??? to ???

A

glyceraldehyde 3-phosphate to 1,3-biphosphoglycerate

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10
Q

NAD+ is a cofactor in which glycolysis step?

A

step 6, produces NADH

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11
Q

step 7: phosphoryl transfer from 1,3-biphosphoglycerate to ??? to turn it into 3-phosphoglycerate and ???

A

ADP to turn it into ATP

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12
Q

step 8: 3-phosphoglycerate is converted to ???

A

2-phosphoglycerate

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13
Q

step 9: dehydration of 2-phosphoglycerate to ???

A

phosphoenolpyruvate

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14
Q

step 10: Transfer of phosphoryl group from phosphoenolpyruvate (PEP) to
ADP produces ??? and ATP

A

pyruvate

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15
Q

step 10 involves the enzyme ???

A

pyruvate kinase

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16
Q

TRUE or FALSE: Overall ATP yield: 2ATP, 2NADH, 2 Pyruvate

A

TRUE

17
Q

glycolysis occurs under aerobic or anaerobic conditions?

A

anaerobic

18
Q

does glycolysis occur in faster rates in tumours? if so, what does this result in?

A

yes, it results in anaerobic metabolism because capillaries can’t provide enough aeration for tumours at the rate they grow.

19
Q

aerobic fate of pyruvate is… which then goes into the TCA cycle

A

Acetyl CoA

20
Q

oxidation of glucose by glycolysis occurs in which part of the cell?

A

cytoplasm

21
Q

how is NADH replenished when not enough oxygen to support aerobic oxidation of pyruvate?

A

NADH is synthesised via the reduction of pyruvate to lactate

22
Q

anaerobic fate of pyruvate is ??? and ???

A

ethanol production and lactate production

23
Q

can humans use the ethanol production pathway in the anaerobic fate of pyruvate? What are we missing?

A

no, humans don’t have pyruvate decarboxylase or alcohol dehydrogenase

24
Q

the two step reduction of pyruvate to ethanol is reversible or irreversible?

A

irreversible

25
Q

Lactate formed by ??? (or by
erythrocytes) can be recycled.

A

active skeletal muscle

26
Q

pyruvate to lactate pathway involves the enzyme…?

A

lactate dehydrogenase