Glucose: Methods of Measurements

Question 1

Curprous ions + arsenomolybdic acid -> arsenomolybdenum blue

Answer 1

Nelson Somogyi

Question 2

Curpous ions + phosphomolybdate -> phosphmolybdic acid / phosphomolybdenum blue

Answer 2

Folin Wu

Question 3

Curprous ions + neocuprine -> cuprous neocuprine complex (yellow/yellow orange)

Answer 3

Neocuprine

Question 4

Modified Folin Wu
Uses citrate/tartrate

Detection and quantitation of reducing substances in body fluids

Answer 4

Benedict’s Method

Question 5

Alkaline Ferric Reduction Method that produces colorless reaction using ferricyande. Inverse colorimetry

Answer 5

Hagedorn Jensen

Question 6

Condensation method that uses glycosylamine and schiff’s base.

Answer 6

Ortho-Toluidine / Dubowski

Question 7

Measures B-D glucose + CSF glucose, falsely increased by BLEACH

Uses Saifer Gernstenfield Method.

Uses glucose oxidase and peroxidase to produce a yellow solution

Answer 7

Glucose Oxidase Method

Question 8

Measures oxygen consumption; uses glucose oxidase in the reaction.

H2O2 is prevented from forming using molybdate, iodide, catalase, and ethanol

Answer 8

Polagrographic - glucose oxidase

Question 9

Reference method for glucose determination. More accurate and specific than glucose oxidase. NADPH measured at 340nm

Answer 9

Hexokinase

Question 10

Results with this method are in close agreement with hexokinase. Mutarotase is added to shorten time necessary to reach equilibrium

Answer 10

Glucose Dehydrogenase