Gluconeogenesis

Question 1

Where does gluconeogenesis occur?

Answer 1

90% liver

10% kidneys

Question 2

When does glucose need to be made?

Answer 2

• times of fasting

- fight/flight

Question 3

What are the 3 reactions that differ between glycolysis and gluconeogenesis?

Answer 3

1) Hexokinase –> G-6-phosphatase (only expressed in liver)
2) Phosphofructokinase –> F-1,6-bisphosphatase
3) Pyruvate kinase –> pyruvate carboxylase + biotin (mitochondria) & PEP carboxylase (cytosolic)

Question 4

What is PEPCK used for in gluconeogenesis?

Answer 4

OAA –> PEP (–> glucose)

Question 5

What stimulates PEPCK?

Answer 5

glucagon

Question 6

What kind of metabolism is the Cori Cycle?

Answer 6

Anaerobic

Question 7

How does the Cori cycle feed into gluconeogenesis?

Answer 7

Lactate (+lactate dehydrogenase)

Question 8

In muscles, how does the high concentration of NADH + H+ get resolved?

Answer 8

produce lactate (Cori Cycle)

Question 9

In the liver, how does the high concentration of NADH + H+ get resolved?

Answer 9

produce pyruvate from lactate (Cori Cycle)