Genetics: Recombinant DNA

Question 1

is an artificially made DNA strand formed by the combination of two or more gene sequences

Answer 1

Recombinant DNA (rDNA)

Question 2

is defined as the direct manipulation of an organism’s genes

Answer 2

Genetic engineering

Question 3

the DNA created by joining together pieces of DNA from diff sources

Answer 3

rDNA

Question 4

refers to the various technique and procedures used in gene manipulation

Answer 4

genetic engineering

Question 5

helps to cut

Answer 5

restriction enzymes

Question 6

helps to synthesize

Answer 6

polymerase

Question 7

helps to bind

Answer 7

ligase

Question 8

helps in carrying and integrating the desired gene

Answer 8

vector

Question 9

most common vectors

Answer 9

plasmids and bacteriophages

Question 10

into which the recombinant DNA is introducted

Answer 10

host organism

Question 11

are DNA ligase and polymerase

Answer 11

modifying enzymes

Question 12

stages in gene cloning

Answer 12

1. Isolation of the target gene
2. Insertion of the target gene into vector
3. Introduction of vector into host
4. Amplification of the target gene

Question 13

2 ways to isolate gene

Answer 13

1. through restriction enzymes
2. through reverse transcription

Question 14

used to detect HIV

Answer 14

DNA technology

Question 15

used as an attemp to correct the gene defect

Answer 15

gene therapy

Question 16

ELISA is an example where the application of rDNA

Answer 16

Clinical diagnosis

Question 17

is widely used in agriculture to produce GMO

Answer 17

rDNA Technology

Question 18

clones are genetically identical as the cell simply replicates producing identical daughter cells

Answer 18

DNA cloning