Genetic Engineering And Genomics Flashcards
In order to sub clone (recombine) a human gene into a plasmid, both must____.
Be cut by the same restriction enzyme.
The unpaired nucleotides produced by restriction enzymes are referred to as….?
Sticky ends
What enzyme forms covalent bonds between restriction fragments?
Ligase?
In gel electrophoresis DNA molecules migrate from ____ to _____ ends of the gel.
Negative….positive
In recombinant DNA experiments ______ is used to cut pieces of DNA and ____ joins the resulting fragments to form recombinant DNA.
A restriction enzyme…..DNA Ligase
A Nucleic acid probe can be used to…?
Find nucleotides within a mass of DNA
How would forensic scientists get a sample if DNA from ancient tissue remains?
Polymerase chain reactions
Sticky ends are useful because..?
They provide sites for complementary base pairing so pieces of DNA can be linked
Bacteria naturally uses restriction enzymes to
Destroy foreign DNA
What is the source of the reverse transcriptase used to produce DNA?
Retroviruses
An enzyme that makes DNA from an RNA template is called?
Reverse transcriptase
In recombinant methods the term vector refers to
A plasmid or other agent used to transfer DNA into a living cell
If a biologist wants to replicate a short segment of DNA in vitro, first she heats DNA to separate the two strands and then…?
Adds nucleotides, primers, and DNA polymerase
Electrophoresis is used to
Separate fragments of DNA
Gene therapy involves
Adding a functioning version of the defective gene to the cells of an individual
