DNA Structure And Replication Flashcards
Who demonstrated DNA acts as the genetic material using T2 bacteriophage?
Hershey and chase
Components of chromosomes?
Nuclei acids and proteins
The radioactive isotope 32P labeled which component do the T2 bacteriophage
DNA
Radioactive isotope 35s labeled which component of the t2 bacteria phage
Protein coat
DNA strands running anti parallel is when
When one strand runs 5’-3’ and one runs 3’-5’
Because of the 2nM width of the DNA double helix only a ______ base can pair with a _____base
Purine, pyrimidine
____NA is double stranded ___NA is single stranded
D, R
Difference in sugar of DNA and RNA?
DNA has 2 oxygen atoms in the sugar, RNA has 3 in the sugar
Purines?
Guanine, adenine
Pyrimidines?
Cytosine, thyamine, uracil.
In a nucleotide the nitrogenous base is attached to the sugars____carbon and the phosphate group is attached to the sugars ____carbon?
1’ 5’
Nucleic acids are assembled in the ___ to ___direction.
5’ 3’
Which enzyme joins short segments (Okazaki fragments) into a continuous strand?
Ligase
Product of DNA replication
2 new double helices each consisting one old and one new DNA strand
The 1st step in DNA replication is catalyzed by what enzyme?
Helicase
The action of helicase creates?
Replication forks and replication bubbles
Why is the new DNA strand complementary to the 3’ 5’ strand assembled in short segments?
DNA polymerase can only be assembled in the 5’3’ direction
The synthesis of a new DNA strand always begin with the synthesis of
A short RNA primer complementary to a preexisting DNA strand
What is responsible to catalyzing the formation of an RNA primer
RNA Pol (primase)
What catalyzes synthesis of new DNA strands?
DNA polymerase
What stabilizes separated DNA strands during replication
Single-strand binding protein
An old DNA strand is used as a ____ for the assembly of a new DNA strand?
Complimentary template.
Weakest bonds in a DNA molecule are between?
Base to base
What type of bonds hold together the sugar phosphate backbone of DNA
Covalent
