Genetic Engineering

Question 1

Insertion describe

Answer 1

Locate plasmid
Cut with same restriction endonuclease
Combine with DNA section using DNA ligase

Question 2

Transformation describe

Answer 2

Place in suitable medium eg bacteria

Question 3

How do we see if bacteria has gene?

Answer 3

Put in ampicillin and those that have take. Up gene will remain

Question 4

Restriction mapping describe

What it used to do?

Answer 4

Cut DNA with restriction endonuclease
Separate by gel electrophoresis
Recognition sites can be determined by fragmentation patterns

Manipulate DNA
Compare fragments

Question 5

How DNA probes used to identify genes

Answer 5

Heat DNA separate two strands 
Mix with gene probe
Cool allow to complimentary base pair 
Radioactive-autoradiography 
UV light-fluorescent electron microscope

Question 6

Isolation describe

Answer 6

Locate DNA- make from mRNA using reverse transcriptase or locate with genetic probe
Cut with restriction endonuclease at recognition sites form sticky ends

Question 7

What’s a DNA probe?

What’s a primer?

Answer 7

Single stranded DNA that complimentary base pairs

Marks beginning or end of DNA needed for complimentary start of gene replication of base from here

Question 8

Describe PCR

Answer 8

Heat to 90 
Strands separate 
Cool 75 
Primers bind 
Nucleotides complimentary base pair 
DNA polymerase join 
Cycle repeated