DNA Flashcards

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1
Q

Semi conservative method describe?

A

Helicase,DNA polymerase and u dwindling proteins bind to strand
Helicase breaks hbonds
Free nucleotides join complimentary base pair
DNA polymerase joins forming new strand
2 new strands formed each with one original

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2
Q

Protein synthesis
What’s a gene also known as and what is its definition?
What’s coded info?
What forms proteins? What are these? What do they control? Therefore what do genes determine?

A

Cistron sections of DNA that code for a polypeptide
Specific sequence of bases along DNA
Polypeptides,enzymes,chemical reactions, nature and development of proteins

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3
Q

Ribosomes
What are they the site of?
What’s the evidence of this?
What does it consist of? What’s in these units?
How was autoradiography used to show ribosome was site of protein synthesis?

A

Protein synthesis
RBC loses nucleus and still synthesises protein
Small and large unit 3 different RNA and many proteins
Put radioactive amino acids into cell places photographic film over and where radioactivity can be seen is site of protein synthesis

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4
Q

Why can DNA not be used directly in protein synthesis?
What happens instead?
The. What passes this to the ribosomes?
Where what occurs?

A

Can’t leave the nucleus
Transcribed
mRNA carry to ribosomes
Translated

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5
Q

What does rRNA do?
What does mRNA do?
What does tRNA do?
What’s the size of each

A

Makes ribosomes
Carry gene and copy info from nucleus
Carries aa in cytoplasm to ribosome

Same size as gene
Varying size
V small

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6
Q

Genetic code
What is it?
How many naturally occurring amino acids are there?
How many nucleotides code for an amino acid? What is this known as?

A

The sequences of bases in DNA that code for the sequence of amino acids that make up a protein
20
3-triplet code

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7
Q

Small pieces of mRNA were added to free systems in a test tube
What’s the advantage?
What’s molecules must be in the test tube?(4)

A

Energy can be used specifically in process not in metabolic processes

Ribosomes,amino acids,ATP and enzymes

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8
Q

If synthetic mRNA contained 18 uracil how many amino acids would it contain?
What’s it called when some amino acids are coded for by more than one codon?
What are stop codons?what do the indicate? What are they also known as?
Why is it called the universal code?
What’s an intron?

A

6
Degenerate code
Codons that do not code for an amino acid, indicate the end of a sequence, non sense codons
Seems to be the same in all living organisms
Sections of DNA that do not code for an amino acid

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9
Q
Where is DNA found?
What's the polymer?
What's the monomer?
What are the strands held by?
How does it replicate?
A
Chromosomes in nucleus of eukaryotic cells
Nucleic acid 
Nucleotide 
Hydrogen bonds 
Semi conservative
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