Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

MT-MLS 208 [PARA LAB] > General Protocol in the Laboratory > Flashcards

General Protocol in the Laboratory Flashcards

1
Q

What is the purpose of general protocol and safety in the laboratory?

A

To ensure that we and the community around us are safe.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Define Aseptic Techniques.

A

Handling specimens considering their contamination.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What does safety refer to in the laboratory context?

A

Condition of protecting ourselves from any type of harm or injury.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is an accident?

A

Any unexpected, unplanned occurrence which may or may not result in injury, property damage, work stoppage, or interference.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Define Occupational Safety.

A

The prevention or control of accidents in the workplace.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is a hazard?

A

Any source of potential damage, harm, or adverse health effects on someone or something.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

List types of hazards.

A
  • Physical
  • Chemical
  • Biological
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What does exposure refer to?

A

The extent to which individuals come into contact with a hazard.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Define risk in the context of hazards.

A

The probability of realizing harm due to an exposure to a hazard.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the RISK FORMULA?

A

Hazard + Exposure = Risk

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What defines unsafe conditions in the workplace?

A

Non-ideal workplace conditions that can cause immediate injuries or accidents.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What are engineering controls?

A

Instruments or machines used to isolate someone from the hazard.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Define unsafe acts.

A

Risky acts such as not following protocols or order of process.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What are occupational health hazards?

A

Factors in the workplace that can lead to long-term health issues.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

List types of occupational health hazards.

A
  • Chemical
  • Physical
  • Biological
  • Radioactive
  • Ergonomic
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the meaning of biological hazards?

A

Biological substances that pose a threat to the health of living organisms.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What is the chain of infection?

A

Requires a continuous link between an infectious agent, a reservoir, a portal of exit, a means of transmission, a portal of entry, and a susceptible host.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What are fomites?

A

Inanimate objects that can carry biological agents.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What are Universal Precautions?

A

All patients are considered to be possible carriers of bloodborne pathogens.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What does Body Substance Isolation (BSI) entail?

A

All body fluids and moist body substances are considered potentially infectious.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What is the major disadvantage of BSI?

A

Does not recommend hand washing after removal of gloves unless visually contaminated.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What is emphasized in the Standard Precautions Guidelines?

A

Hand hygiene.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What does proper handwashing procedure include?

A
  • Wet hands with warm water
  • Apply soap
  • Rub to form lather
  • Rinse hands
  • Dry with paper towel
  • Turn off faucets with a paper towel
24
Q

What should be done for biologic waste disposal?

A

All biologic waste must be placed in appropriate containers labeled with the biohazard symbol.

25
What should be done in case of chemical spills?
Flush the area with large amounts of water for at least 15 minutes then seek medical attention.
26
What is the Chemical Hygiene Plan (CHP)?
A written plan required by OSHA for facilities using hazardous chemicals.
27
What does the NFPA 704 system identify?
The fire hazards of materials.
28
What are the steps during a fire? (RACE)
* Rescue * Alarm * Contain * Extinguish/Evacuate
29
What does the PASS acronym stand for?
* Pull the pin * Aim at the base of the fire * Squeeze handles * Sweep nozzle side to side
30
What are protective procedures in the laboratory?
* Wear personal protective equipment * Use appropriate pipetting devices * Tie long hair back securely
31
What should be done in case of electrical shock?
Remove the electrical source immediately without touching the person or equipment.
32
What are the emergency procedures in the laboratory?
Locate and use emergency equipment properly.
33
What should be done with blood samples?
All blood samples are considered potentially infectious.
34
What is the guideline for chemical handling?
Chemicals should never be mixed together unless specific instructions are followed.
35
What should you do for skin contact with chemicals?
Flush the area with large amounts of water for at least 15 minutes.
36
What is the proper procedure for cleaning up biological spills?
* Don PPE * Prepare disinfectant * Cover the spill * Pour disinfectant * Allow contact time * Dispose of materials in biohazard bags
37
What must all electrical equipment be?
Grounded with three-pronged plugs.
38
What is the proper way to dispose of sharp objects?
Dispose in puncture-resistant, leak-proof containers with the biohazard symbol.
39
What should be done with microscope slides used in parasitological procedures?
Place slides in a pot container with 1% hypochlorite solution and bury in a disposable specimen container ## Footnote This is to ensure proper disposal and decontamination of potentially infectious materials.
40
What are the restrictions regarding personal items in the laboratory?
Belongings must be placed in the designated area to avoid obstruction of workflow ## Footnote Keeping the laboratory free of unnecessary articles ensures a safer working environment.
41
What actions are prohibited in the laboratory?
Eating, drinking, and smoking are FORBIDDEN at all times ## Footnote These actions can lead to contamination and exposure to hazardous materials.
42
What is the purpose of wearing a laboratory coat or smock?
To protect clothing from contamination or accidental discoloration by reagents and staining solutions ## Footnote Protective clothing is essential for maintaining safety in the laboratory.
43
What should be done if infectious material is accidentally spilled?
Cover it immediately with a paper towel dampened with 5% Lysol or 5% phenol, and notify the instructor immediately ## Footnote Quick action is crucial to minimize exposure and contamination.
44
What are universal precautions?
Universal precautions are guidelines to prevent exposure to HIV and other blood/fluids-borne pathogens by treating all biological samples as potentially infectious ## Footnote This approach is essential in healthcare settings to protect workers and patients.
45
What protective equipment should be worn during procedures likely to generate aerosols?
Masks, protective goggles, or face shields should be worn ## Footnote These items protect mucous membranes from exposure.
46
What should be done with used needles and sharp instruments?
They should be discarded in a puncture-resistant container for disposal ## Footnote Proper disposal reduces the risk of needlestick injuries.
47
True or False: Saliva is a known transmission route for HIV.
False ## Footnote While saliva is not a recognized transmission route, precautions should still be taken.
48
What should be done to clean up spills of biological fluids?
Wear PPE, wipe excess materials with disposable towels, and disinfect the area ## Footnote Following these steps ensures safety and prevents contamination.
49
What type of container should biological fluids be placed in for transport?
A well-constructed container with a secure lid to prevent leaks ## Footnote Proper containment is crucial for safety during transport.
50
What should be worn when processing biological fluids?
Gloves, and masks and protective eyewear if mucous membrane contact is anticipated ## Footnote Personal protective equipment is vital for safeguarding health.
51
Fill in the blank: Mouth pipetting is _______.
NOT ALLOWED ## Footnote This practice is banned to prevent accidental ingestion of hazardous materials.
52
What should be done with contaminated materials used in laboratory tests?
They should be decontaminated before reprocessing or disposed of according to institutional policies ## Footnote Proper disposal of hazardous materials is crucial for safety.
53
What is the recommended procedure after completing laboratory activities?
Wash hands and remove protective clothing before leaving the premises ## Footnote This helps to prevent contamination outside the laboratory.
54
What type of cabinet should be used for high-risk aerosol-generating procedures?
Biological safety cabinets should be used ## Footnote These cabinets provide additional protection from airborne pathogens.
55
What should be used to decontaminate laboratory work surfaces after a biological fluid spill?
An appropriate chemical germicide ## Footnote Decontamination is essential for maintaining a safe laboratory environment.

MT-MLS 208 [PARA LAB] (7 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions