Genera Treponema, Brachyspira, and Borrelia Flashcards
Treponema and Brachyspira are _____ organisms that can act as _____ or ______
Anaerobic
Commensals
Parasites
What would you use to stain Treponema and Brachyspira?
Crystal violet and/or Giemsa stain
_____ was originally thought to be the only cause of swine dysentary
Brachyspira hyodysenteriae
What is swine dysentary?
Muco-hemorrhagic diarrhea that can affect any age pig, but seen primarily in grower pigs
What kind of Brachyspira are the most capable of producing disease?
Beta-hemolytic strands
What do the colonies of Brachyspira look like?
More of a film than a distinct colony, with some producing a strong zone of beta hemolysis
The ring phenomenon is characteristic of what species?
Brachyspira hyodysenteriae
What is the transmission of Brachyspira hyodysenteriae?
Pig to Pig via fecal oral transmission
T/F Brachyspira hyodysenteriae can live in lagoons for up to a year and is very difficult to get out of a swine unit
TRUE
Describe the pathogenesis of Brachyspira hyodysenteriae
Incubation period of 7-10 days
Organism doesn’t penetrate past lamina propria and only is in large intestinal areas
Fecal material can become very watery and bloody
Epithelial lining can become so involved it produces a pseudomembrane
Pigs completely recover 1-2 months after initial disease
Pigs are immune after infection
What are the 3 virulence factors of Brachyspira hyodysenteriae?
Hemolysin
LPS
Attachment: Suspected, but not proven
What can be used to diagnose Brachyspira hyodysenteriae?
Good history is important; intro of new stock purchased at a sale barn
Clinical signs and gross lesions
Isolation and identification to demonstrate the organism
What is the guidelines for isolating a good specimen?
Acutely ill, non-medicated pig
Is immunization against Brachyspira hyodysenteriae done?
No; elimination of the organism from the facility is the only true way to rid this disease
________ causes porcine intestinal spirochetosis and human intestinal spirochetosis
Brachyspira pilosicoli
What is the distribution of Brachyspira pilosicoli?
Worldwide in swine and humans
Brachyspira pilosicoli is a _____ beta-hemolytic intestinal spirochete
weakly
What is the growth of Brachyspira pilosicoli?
Similar to Brachyspira hyodysenteriae. The only difference is that it is a weakly beta hemolytic organism.
Describe the disease Brachyspira pilosicoli causes in pigs
Attaches in large numbers to the colonic epithelium by one end of the bacterial cell
Produces a diarrheal disease in immediate postweaning pigs, diarrhea with occasional flecks of blood
In general the disease is much milder
Describe the disease Brachyspira pilosicoli
Also characterized by teh end on attachment in the large intestine.
Common in underdeveloped countries and AIDs patients
Can see rectal bleeding with chronic diarrhea