gene regulation and the trp operon Flashcards
gene
a section of a strand of DNA, contains information to code for a protein
regulatory sequence
section of DNA upstream of a gene that is capable of increasing or decreasing the expression of specific genes within an organism.
promoter region
where RNA polymerase binds
enhancer/silencer
regionwheretranscription factors can bind
UTR
stands for untranslated region located on each side of a coding sequence (this section is transcribed but not translated)
promoter region
are sections of a gene that are found before the start triplet on the 5’ end of DNA strand
Promotors
determine the site of transcription and direct the binding of
RNA polymerase
• Structural genes
genes that code for proteins that become part of the structure and functioning of an organism.
• The proteins produced by structural genes do not control other genes
operon
stands for a group of genes with a single promotor and leader meaning that all the genes in the group are transcribed as a single mRNA molecule
operator
a short DNA segment that forms the binding site for a transcription factor, in this case trp repressor
leader
sequence of nucleotide bases with regulatory activity
attenuator
mRNA transcription termination site made up of multiple Adenine-
Uracil base pairs
trp operon
The regulation of the trp operon is determined by the concentration of tryptophan. Transcription is turned off when a high concentration of tryptophan is present, and is turned on when tryptophan is absent.
two levels of regulation
- transcription repression
- attenuation