CRISPR Flashcards
what does CRISPR stand for
cluster regularly interspaced short palindromic repeats
spacer DNA
unique sequences of DNA nestled in between each palindromic repeat.
where is CRISPR found
bacteria and other prokaryotes
Cas genes
encode Cas proteins which are helicases that cause DNA to unwind which enables endonucleases to cut the DNA
which system is CRISPR apart of
adaptive immune system
Adaptive immunity
immunity an organism acquires after exposure to a pathogen
bacteriophage
type of virus that infects its host bacterial cell by injecting its genetic material into the host cell, taking over the cell to make replicates of itself and then releasing those replicated bacteriophages ultimately killing the cell.
protospacer adjacent motif (PAM)
located about 2-6 nucleotides downstream of the target DNA sequence that is to be cleaved by the Cas nuclease