gene linkage and sordoria

Question 1

What is the most diverse fungal phylum?

Answer 1

Phylum Ascomycota

Question 2

What is the ascus?

Answer 2

The defining feature in a microscopic sexually structure

Question 3

Where is the ascospores formed?

Answer 3

In the ascus and form in a linear order

Question 4

What did Lindsay Olive discover?

Answer 4

Found that ascospore color is determined by the genotype of the spore.

Question 5

What way do they reproduce?

Answer 5

both sexually and asexually

Question 6

Each haploids spore’s phenotype is determined by what?

Answer 6

The spore colored allele it posesses

Question 7

What is Plasmogamy?

Answer 7

The fusion of two hyphae together

Question 8

What is Dikaryotic stage?

Answer 8

Cells have two haploid nuclei

Question 9

What is Karyogamy?

Answer 9

Nuclei fuse together and create one diploid nucleus

Question 10

What is meiosis in ascomycetes?

Answer 10

Results in four cells haploid ascospores

Question 11

What is mitosis in ascomycetes?

Answer 11

Cells divide to form eight ascospores in an ascus

Question 12

What is dispersal?

Answer 12

Mature ascus release the ascospores to start a new mycelium

Question 13

What is sodaria fimicola?

Answer 13

Microscopic ascomycete that has a life cycle of 1-2 weeks. Spends most of its life in a haploid vegetative state.

Question 14

What is tetrad analysis?

Answer 14

Tetrad refers to the four spores created after meiosis in the Ascomycota life cycle. allows us to follow the chromatid throughout the meiotic process.

Question 15

What is gene linkage?

Answer 15

The tendency of genes that are located close to each other on the same chromosome to be inherited together during meiosis

Question 16

What does tetrad analysis help us find?

Answer 16

1. Helps us identify 2 of the 4 chromatids that participate in crossing over.
2. The mapping of gene markers close to the centromere of the chromosomes

Question 17

When traits are frequently seen inherited together we can create a what?

Answer 17

genetic map

Question 18

What is genetic mapping?

Answer 18

When 2 traits are regularly inherited together, therefore we can determine that the genes are close together on a chromosome

Question 19

What are the ways to make a linkage map?

Answer 19

Crossbreeding, tracking traits, or recombination frequency

Question 20

What is the recombination frequency?

Answer 20

recombinants/total offsprings x 100%

Question 21

Sordaria is good to use in labs for what experiments?

Answer 21

For conducting tetrad analysis for the segregation for spore colored mutants

Question 22

How is ascospore color determined?

Answer 22

By the genotype of the spore. (It can be observed in the ascus)

Question 23

Even during sexual reproduction does the fungi have to undergo meiosis before mitosis?

Answer 23

yes

Question 24

Sordoria (ascomycetes) is all what cells?

Answer 24

haploid cells

Question 25

What is produced in mitosis for the sordoria?

Answer 25

4 spores of each type (8 total) this shows gene linkage

Question 26

When the ratio is 4:4 that means what?

Answer 26

no crossing over occurred

Question 27

When the ratio is 2:2:2:2 that means what?

Answer 27

Crossing over has occurred and recombinant chromosomes has been produced

Question 28

How can we count the asci?

Answer 28

By putting them under a glass slide and popping them. We can then count them under a microscope

Question 29

Who discovered crossing over?

Answer 29

Thomas Morgan Hunt

Question 30

How to find the percent of crossing over?

Answer 30

(total cross over/total number) = # then #/2

the percent will also be the gene distance number

Question 31

What is the accepted value of crossing over?

Answer 31

26%

Question 32

What are some examples of fungal phylum?

Answer 32

bakers yeast, truffles, and morrels