cancer Flashcards

1
Q

What is genetic testing?

A

Its any test that is capable of identifying changes in DNA

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2
Q

Genetic testing utilizes what types of samples?

A

spit and blood

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3
Q

Why do people get genetic testing?

A

To see if there are any genetic conditions that they need to prepare for, to see if they inherited a genetic disease, to diagnose a genetic disease, and create a treatment plan

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4
Q

Is genetic testing on a singular gene or the whole genome?

A

both

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5
Q

True or false. Creating a pedigree is done before getting genetic testing

A

true

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6
Q

What do genetic counselors do?

A

They use information from pedigrees and recognize patterns of inheritance and decide which individuals benefit form genetic testing.

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7
Q

What do pedigree’s show?

A

The relationship between individuals and affected family members.

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8
Q

What is hereditary colon cancer?

A

(HNPCC) It is hereditary nonpolyposis colorectal cancer and its 5%of all diagnose colon cancers.

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9
Q

What causes HNPCC?

A

One of 4 mutated caretaker or mismatch repair genes. (MLH1, MSH2, MSH6, PMS2). it is a recessive disease

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10
Q

What is a caretaker gene?

A

They scan the genome to help repair DNA mutations

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11
Q

Does inheriting one of the mutated genes guarantee your get HNPCC?

A

no only 80% chance and occurs when both parents have the mutated gene.

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12
Q

True or false. cancer can start in most cell types

A

true

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13
Q

True or false. cancer is the 2nd leading cause of death behind heart disease

A

true

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14
Q

What is the colon?

A

It is the main part of the large instesitnes responsible from removing water and electrolytes from food and storing waste until it passes out the body.

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15
Q

What percent of cancers are genetically inherited?

A

5%

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16
Q

What is cancer in the rectum?

A

rectal cancer

17
Q

What is cancer in the colon

A

colon cancer

18
Q

What is the cancer in both the colon and rectal regions?

A

Colorectal cancer

19
Q

Doctors used to observe cells from what compared to know testing gene activity

A

fecal samples

20
Q

What is gel electrophoresis?

A

It is a method of separating DNA, RNA, and proteins by size and charge.

21
Q

What molecules move faster in the gel?

A

More negative molecules move faster

22
Q

What molecules move easier in the gel?

A

Smaller molecules move and quickly than larger ones

23
Q

Banding seen on gels is a result of what?

A

restriction enzymes

24
Q

What are bacteriophages?

A

They are viruses specialized for infecting bacteria by injecting their DNA into the bacteria’s cytoplasm.

25
Q

Why does bacteria produce restriction enzymes?

A

To recognize and cleave viral DNA

26
Q

What do restriction enzymes normally recognize?

A

palindromes which accounts for differences in weight if sample load to gels.

27
Q

The electric current of the gel will run what way?

A

From negative to positive

28
Q

Can you put your hand in the buffer when the gel is running?

A

NO

29
Q

True or false. The gel can be placed or cast in the apparatus

A
30
Q

What do we use for serial dilutions?

A

C1V1=C2V2
C = concentration
V = volume