Gene Editing Flashcards

1
Q

What does a CRISPR/Cas9 plasmid contain?

A

Promotor, guide RNA, CRISPR RNA, another promotor, CAS9 gene, fluorescence gene.

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2
Q

How is the bacteria CAS9 modified to engineering use?

A

Added nuclear signalling sequence.

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3
Q

What must follow the DNA sequence targeted by CRISPR/Cas9?

A

Protospacer adjacent motif (PAM).

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4
Q

What is the protospacer adjacent motif?

A

Any nucleotide followed by GG.

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5
Q

What is the size of the DNA sequence recognised by a pair of Z-fingers, TALENs and CRISPR/Cas9?

A

18-36, 28-40, 22, respectively.

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6
Q

What are the trageting Z-fingers, TALENs and CRISPR/Cas9?

A

Z-fingers: Difficult to target non-G-rich sequences
TALENs: 5’ targeted base must be T
CRISPR: PAM site

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7
Q

List Z-fingers, TALENs and CRISPR/Cas9 in decreasing order of difficulty to engineer.

A

Z-fingers, TALENs and CRISPR/Cas9.

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8
Q

How can Z-fingers, TALENs and CRISPR/Cas9 be delivered ex-vivo?

A

Electroporation and viral transduction.

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9
Q

List three disease that can be treated with Z-fingers.

A

Haemophilia B, HIV, SCID

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10
Q

List three diseases that can be treated with TALENs

A

DMD, Hep B, B-cell accute lymphoblastic leukaemia.

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11
Q

List seven disease that can potentially be treated with CRISPR/Cas9.

A

HIV, DMD, cataracts, cystic fibrosis, hereditary tyrosinemia.

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